ID   CEF1_CRYNB              Reviewed;         833 AA.
AC   P0CO95; Q55JY9; Q5K9L1;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=Pre-mRNA-splicing factor CEF1;
GN   Name=CEF1; OrderedLocusNames=CNBK1950;
OS   Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
OS   (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=283643;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B-3501A;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Involved in pre-mRNA splicing and cell cycle control.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Associated with the spliceosome. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus
CC       {ECO:0000255|PROSITE-ProRule:PRU00625}.
CC   -!- SIMILARITY: Belongs to the CEF1 family. {ECO:0000305}.
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DR   EMBL; AAEY01000052; EAL18176.1; -; Genomic_DNA.
DR   RefSeq; XP_772823.1; XM_767730.1.
DR   AlphaFoldDB; P0CO95; -.
DR   SMR; P0CO95; -.
DR   EnsemblFungi; EAL18176; EAL18176; CNBK1950.
DR   GeneID; 4938892; -.
DR   KEGG; cnb:CNBK1950; -.
DR   VEuPathDB; FungiDB:CNBK1950; -.
DR   HOGENOM; CLU_009082_0_0_1; -.
DR   Proteomes; UP000001435; Chromosome 11.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   CDD; cd00167; SANT; 1.
DR   InterPro; IPR021786; Cdc5p/Cef1_C.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017930; Myb_dom.
DR   InterPro; IPR001005; SANT/Myb.
DR   Pfam; PF11831; Myb_Cef; 1.
DR   Pfam; PF00249; Myb_DNA-binding; 2.
DR   SMART; SM00717; SANT; 2.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS51294; HTH_MYB; 2.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; DNA-binding; mRNA processing; mRNA splicing;
KW   Nucleus; Repeat; Spliceosome.
FT   CHAIN           1..833
FT                   /note="Pre-mRNA-splicing factor CEF1"
FT                   /id="PRO_0000410154"
FT   DOMAIN          2..57
FT                   /note="HTH myb-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   DOMAIN          58..102
FT                   /note="HTH myb-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   DNA_BIND        30..53
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   DNA_BIND        76..98
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   REGION          109..150
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          248..280
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          428..447
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          480..534
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          245..306
FT                   /evidence="ECO:0000255"
FT   COILED          722..808
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        250..273
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        492..511
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        512..534
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   833 AA;  92625 MW;  35573919A21B7281 CRC64;
     MRVIVKGGVW RNTEDEILKA AISKYGKNQW ARISSLLVRK TPKQCKARWY EWLDPSIKKT
     EDEKLLHLAK LMPTQWRTIA PIVGRTATQC LERYQKLLDD AEARDNEELG LGAGEDESSK
     PATDARGLRP GEIDTDPETR PARPDPIDMD DDEKEMLSEA RARLANTQGK KAKRKARERQ
     LEEARRLAFL QKKRELKAAG INLRAKPKKK GMDYNADIPF EKQPAPGFYD VTEEQAKVHA
     APVGSTLRAL EGKRKQELDE IEERKKRQKK GDGKSNQTQQ FVAAREAQIK KLKEQEQIIR
     RRKLNLPIPQ VGERELEDIV KIGQAGELAR ELVGDGNKAT EGLLGEYEAL GQAKMARTPR
     TAPQQDNVMA EARNLRNMMA AQTPLLGEEN TPLHGPSVGT GFEGATPRHD VAATPNPLAT
     SARGGVLTST RTVPGVGTTP LRTPFRDDLN INDDASVYGE TPMNDRRRLA ESRRALKAGF
     AALPKPENNF ELAETEEDEE EAEEAEPLTE EDAAERDARL KAAREEEERR ELERRSTVIK
     KGLPRPVNVN TYKLLDDLNS AIVEQTDEEM AAAFKLVNLE VAMLMKHDSI AHPLPGTSTP
     GGLASEYDMP EDDFVAEAKN AIHTELANAL GLPGASDEHL RLAIGAAAEE NEAAFAEAWA
     EEREGLVYSP STRTWVDKTS LSPEELSACY AAMINASRDR VIAEATKAAK AEKKLGKQLG
     GYQTLNEKAK KAIVDVMEEI HQTKRDMETF LMLKGIEEAA APARLEKIRE EVAVLEKRER
     DLQARYAELN DRRRENLAAI EQLEEDKIVL AAQVALEAQE GEVADGDVDM NGA