ID   CEF1_KLULA              Reviewed;         543 AA.
AC   Q6CU65;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 100.
DE   RecName: Full=Pre-mRNA-splicing factor CEF1;
GN   Name=CEF1; OrderedLocusNames=KLLA0C07304g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Involved in pre-mRNA splicing and cell cycle control.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Associated with the spliceosome. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus
CC       {ECO:0000255|PROSITE-ProRule:PRU00625}.
CC   -!- SIMILARITY: Belongs to the CEF1 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CR382123; CAH01375.1; -; Genomic_DNA.
DR   RefSeq; XP_452524.1; XM_452524.1.
DR   AlphaFoldDB; Q6CU65; -.
DR   SMR; Q6CU65; -.
DR   STRING; 28985.XP_452524.1; -.
DR   EnsemblFungi; CAH01375; CAH01375; KLLA0_C07304g.
DR   GeneID; 2892559; -.
DR   KEGG; kla:KLLA0_C07304g; -.
DR   eggNOG; KOG0050; Eukaryota.
DR   HOGENOM; CLU_009082_2_1_1; -.
DR   InParanoid; Q6CU65; -.
DR   Proteomes; UP000000598; Chromosome C.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000974; C:Prp19 complex; IEA:EnsemblFungi.
DR   GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; IEA:EnsemblFungi.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000386; F:second spliceosomal transesterification activity; IEA:EnsemblFungi.
DR   GO; GO:0000350; P:generation of catalytic spliceosome for second transesterification step; IEA:EnsemblFungi.
DR   CDD; cd00167; SANT; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017930; Myb_dom.
DR   InterPro; IPR001005; SANT/Myb.
DR   SMART; SM00717; SANT; 2.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS51294; HTH_MYB; 1.
DR   PROSITE; PS50090; MYB_LIKE; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; DNA-binding; mRNA processing; mRNA splicing;
KW   Nucleus; Reference proteome; Repeat; Spliceosome.
FT   CHAIN           1..543
FT                   /note="Pre-mRNA-splicing factor CEF1"
FT                   /id="PRO_0000197101"
FT   DOMAIN          1..58
FT                   /note="HTH myb-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   DOMAIN          59..106
FT                   /note="Myb-like"
FT   DNA_BIND        33..56
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   REGION          256..275
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          135..188
FT                   /evidence="ECO:0000255"
FT   COILED          459..541
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   543 AA;  62296 MW;  FE812C43EA03D6A9 CRC64;
     MAPVPIYVKG GIWTNLEDQI LKAAVQKYGT HAWSKVASLL QKKNARQCQS RWNEFLNPSL
     NFKPFSQQED DKLLDLAKRI PNQWKSIGEM MGRPAQTCID RYNILLAIDK DDLELAATTG
     VQIGEINPNN ESLPAKADKD ELLDEEREML AEARARLLNT QGKKATRKIR ERMLEESKRV
     AFLQKRRELK QAGVDSKIKA PRKKYSSQMD YNEDIAYEQQ PLPGIYDTSK EDEQTEKLLK
     SFEKLVERKG LLDRKSDEKK TIPQKRRQGE QITSSVTKLD SVLTDEYKKP KLELTPATLK
     GSKRKINKTT SILHLFESLP SPKNDFEIVL DDLDEEMEST DAFNTESSYV NGKYPLEALI
     SSTEHQSENK AMEIMEYIPT VFQIKTELQV PSPIVDPKNE IDRELNRIIS AKENGNPIYN
     STEVNNYLEE IINSASTTEV PSTVLHPKLE TSLKTITPAT EHELLGMERK VHELRTALED
     SVKSHREAVS QLSQDFEEKL HPTVSGLAYQ YYTDYMKYRN DHKTMQEETQ SLAEQIKLAQ
     RSQ