ID   CEFD2_ACRCH             Reviewed;         383 AA.
AC   Q8J0F0;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Isopenicillin N epimerase component 2;
DE            Short=IPN epimerase component 2;
DE            EC=5.1.1.17;
DE   AltName: Full=Isopenicillin N epimerase acyl-CoA racemase component;
DE            EC=5.1.99.-;
GN   Name=cefD2;
OS   Acremonium chrysogenum (Cephalosporium acremonium).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Hypocreales incertae sedis; Acremonium.
OX   NCBI_TaxID=5044;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 48278 / C10;
RX   PubMed=12228250; DOI=10.1074/jbc.m207482200;
RA   Ullan R.V., Casqueiro J., Banuelos O., Fernandez F.J., Gutierrez S.,
RA   Martin J.F.;
RT   "A novel epimerization system in fungal secondary metabolism involved in
RT   the conversion of isopenicillin N into penicillin N in Acremonium
RT   chrysogenum.";
RL   J. Biol. Chem. 277:46216-46225(2002).
CC   -!- FUNCTION: Together with cefD1, catalyzes the reversible isomerization
CC       between isopenicillin N and penicillin N. This two-component IPN
CC       epimerase system may function by two sequential steps, an activation of
CC       isopenicillin N by the acyl-CoA synthase component cefD1, followed by
CC       epimerization by the acyl-CoA racemase component cefD2.
CC       {ECO:0000269|PubMed:12228250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=isopenicillin N = penicillin N; Xref=Rhea:RHEA:20033,
CC         ChEBI:CHEBI:58399, ChEBI:CHEBI:58408; EC=5.1.1.17;
CC   -!- PATHWAY: Antibiotic biosynthesis; cephalosporin C biosynthesis.
CC   -!- SIMILARITY: Belongs to the CoA-transferase III family. {ECO:0000305}.
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DR   EMBL; AJ507632; CAD45624.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8J0F0; -.
DR   SMR; Q8J0F0; -.
DR   UniPathway; UPA00172; -.
DR   GO; GO:0045439; F:isopenicillin-N epimerase activity; IMP:UniProtKB.
DR   GO; GO:0042318; P:penicillin biosynthetic process; IMP:UniProtKB.
DR   Gene3D; 3.30.1540.10; -; 1.
DR   Gene3D; 3.40.50.10540; -; 1.
DR   InterPro; IPR003673; CoA-Trfase_fam_III.
DR   InterPro; IPR044855; CoA-Trfase_III_dom3_sf.
DR   InterPro; IPR023606; CoA-Trfase_III_dom_1_sf.
DR   Pfam; PF02515; CoA_transf_3; 1.
DR   SUPFAM; SSF89796; SSF89796; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Isomerase.
FT   CHAIN           1..383
FT                   /note="Isopenicillin N epimerase component 2"
FT                   /id="PRO_0000418514"
FT   ACT_SITE        158
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   383 AA;  41397 MW;  64179554E671E209 CRC64;
     MDPSRPHPLS GKLVVELAGL APGPFCGMLL ADYGASVLRI DGPRSPKGDV LARNKSSICI
     DLKHPPSRKV LLSILSRADV LIDPFRPGVL ERLGLSPTEV LLKANARLVV ARLTGFRRDG
     KYQDMAGHDI NYLAVSGVLA MLGRAGENPF PPANILGDFA GGGAMCVVGI LLALVSRDAT
     GLGQVVEANM VDGSAYLATM PRLATKTPFW GSPRGENVLD GGCPWYATYR TKDPGGKYMA
     VGALEPHFYE VLVRGLGLDK TDLPPREDRA NWPRLRALFE AKFAERTRSE WAEVFDGTDA
     CVTPVLEQGE LEKAGFEQRL PVNLGATPGK PILPGQGDWT GRTLAKGHGG EEILRRWIGW
     ERGVDYHVEE NSGILVACSR EKL