ID   CEFE_AMYLA              Reviewed;         314 AA.
AC   Q03047;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Deacetoxycephalosporin C synthase;
DE            Short=DAOCS;
DE            EC=1.14.20.1;
DE   AltName: Full=Expandase;
GN   Name=cefE;
OS   Amycolatopsis lactamdurans (Nocardia lactamdurans).
OC   Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC   Amycolatopsis.
OX   NCBI_TaxID=1913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8437592; DOI=10.1007/bf00277148;
RA   Coque J.J.R., Martin J.F., Liras P.;
RT   "Characterization and expression in Streptomyces lividans of cefD and cefE
RT   genes from Nocardia lactamdurans: the organization of the cephamycin gene
RT   cluster differs from that in Streptomyces clavuligerus.";
RL   Mol. Gen. Genet. 236:453-458(1993).
CC   -!- FUNCTION: Catalyzes the step from penicillin N to deacetoxy-
CC       cephalosporin C.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-oxoglutarate + O2 + penicillin N = CO2 +
CC         deacetoxycephalosporin C + H2O + succinate; Xref=Rhea:RHEA:20748,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:30031, ChEBI:CHEBI:58408,
CC         ChEBI:CHEBI:58415; EC=1.14.20.1;
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC   -!- COFACTOR:
CC       Name=L-ascorbate; Xref=ChEBI:CHEBI:38290;
CC   -!- PATHWAY: Antibiotic biosynthesis; cephalosporin C biosynthesis.
CC   -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC       family. {ECO:0000305}.
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DR   EMBL; Z13974; CAA78376.1; -; Genomic_DNA.
DR   PIR; S30900; S30900.
DR   AlphaFoldDB; Q03047; -.
DR   SMR; Q03047; -.
DR   BRENDA; 1.14.20.1; 311.
DR   UniPathway; UPA00172; -.
DR   GO; GO:0050599; F:deacetoxycephalosporin-C synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.330; -; 1.
DR   InterPro; IPR026992; DIOX_N.
DR   InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR   InterPro; IPR027443; IPNS-like_sf.
DR   InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR   Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR   Pfam; PF14226; DIOX_N; 1.
DR   PROSITE; PS51471; FE2OG_OXY; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Iron; Oxidoreductase; Vitamin C.
FT   CHAIN           1..314
FT                   /note="Deacetoxycephalosporin C synthase"
FT                   /id="PRO_0000219510"
FT   DOMAIN          156..269
FT                   /note="Fe2OG dioxygenase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
SQ   SEQUENCE   314 AA;  34530 MW;  D007AD3DE67A1BDF CRC64;
     MTDATVPTFD LAELREGLHQ EEFRHCLREK GVFYLKGTGL PAEADHASGR EIAVDFFDHG
     TEAEKKAVMT PIPTIRRGYA GLESESTAQI TNTGKYTDYS MSYSMGTADN LFPSAEFEKA
     WEDYFARMYR ASQDVARQVL TSVGAEPEVG MDAFLDCEPL LRLRYFPEVP EDRVAEEQPL
     RMAPHYDLSI VTLIHQTPCA NGFVSLQVEV DGSYVDIPAQ PGAVLVFCGA VATLVADGAI
     KAPKHHVAAP GADKRVGSSR TSSVFFLRPN GDFRFSVPRA RECGFDVSIP AETATFDDWI
     GGNYINIRKT AAAR