ID   CEFF_AMYLA              Reviewed;         310 AA.
AC   P42219;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Deacetoxycephalosporin C hydroxylase;
DE            EC=1.14.11.26;
DE   AltName: Full=Beta-lactam hydroxylase;
DE   AltName: Full=Deacetylcephalosporin C synthase;
DE            Short=DACS;
GN   Name=cefF;
OS   Amycolatopsis lactamdurans (Nocardia lactamdurans).
OC   Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC   Amycolatopsis.
OX   NCBI_TaxID=1913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8703431; DOI=10.1007/bf00172492;
RA   Coque J., Enguita F.J., Cardoza R.E., Martin J.F., Liras P.;
RT   "Characterization of the cefF gene of Nocardia lactamdurans encoding a 3'-
RT   methylcephem hydroxylase different from the 7-cephem hydroxylase.";
RL   Appl. Microbiol. Biotechnol. 44:605-609(1996).
CC   -!- FUNCTION: Hydroxylation of desacetoxicephalosporin C in 3'position to
CC       form deacetylcephalosporin C.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-oxoglutarate + deacetoxycephalosporin C + O2 = CO2 +
CC         deacetylcephalosporin C + succinate; Xref=Rhea:RHEA:16805,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:16810,
CC         ChEBI:CHEBI:30031, ChEBI:CHEBI:58366, ChEBI:CHEBI:58415;
CC         EC=1.14.11.26;
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC   -!- PATHWAY: Antibiotic biosynthesis; cephalosporin C biosynthesis.
CC   -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC       family. {ECO:0000305}.
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DR   EMBL; Z21687; CAA79803.1; -; Genomic_DNA.
DR   PIR; S40253; S40253.
DR   AlphaFoldDB; P42219; -.
DR   SMR; P42219; -.
DR   KEGG; ag:CAA79803; -.
DR   UniPathway; UPA00172; -.
DR   GO; GO:0045442; F:deacetoxycephalosporin-C hydroxylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.330; -; 1.
DR   InterPro; IPR026992; DIOX_N.
DR   InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR   InterPro; IPR027443; IPNS-like_sf.
DR   InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR   Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR   Pfam; PF14226; DIOX_N; 1.
DR   PROSITE; PS51471; FE2OG_OXY; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Iron; Oxidoreductase; Vitamin C.
FT   CHAIN           1..310
FT                   /note="Deacetoxycephalosporin C hydroxylase"
FT                   /id="PRO_0000219512"
FT   DOMAIN          154..267
FT                   /note="Fe2OG dioxygenase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
SQ   SEQUENCE   310 AA;  34364 MW;  9983EF1AC1B416E6 CRC64;
     MSDKTVPVFS MAELRDGSRQ DEFREWARRG VFYLTGYGAT ERDHRVATDT AMDFFAQGTA
     EEKQAVTTKV PTMRRGYSAL EAESTAQVTN TGTYTDYSMS YSMGIGGNLF PSKEFESVWT
     DYFDSLYRAA QETARLVLTA AGTYDGEDLD TLLDCDPVLR LRYFPEVPEH RAAEYEPRRM
     APHYDLSIIT FIHQTPCANG FVSLQAEVDG EMVSLPHVED AVVVLCGAIA PLVTQGAVPA
     PNHHVVSPDA SMLKGSDRTS SVFFLRPSTD FTFSVPDARK YGLDVSLDME KATFGDWIGT
     NYVTMHAVTS