CEH28_CAEEL
ID CEH28_CAEEL Reviewed; 201 AA.
AC G5EE18;
DT 10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Homeobox protein ceh-28 {ECO:0000305};
DE AltName: Full=NK-2 homeodomain factor CEH-28 {ECO:0000303|PubMed:25474681};
GN Name=ceh-28 {ECO:0000312|WormBase:K03A11.3};
GN ORFNames=K03A11.3 {ECO:0000312|WormBase:K03A11.3};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:AAO46042.2}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=18161854; DOI=10.1002/dneu.20599;
RA Ray P., Schnabel R., Okkema P.G.;
RT "Behavioral and synaptic defects in C. elegans lacking the NK-2 homeobox
RT gene ceh-28.";
RL Dev. Neurobiol. 68:421-433(2008).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24690231; DOI=10.1016/j.ydbio.2014.03.015;
RA Ramakrishnan K., Ray P., Okkema P.G.;
RT "CEH-28 activates dbl-1 expression and TGF-beta signaling in the C. elegans
RT M4 neuron.";
RL Dev. Biol. 390:149-159(2014).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=25474681; DOI=10.1371/journal.pone.0113893;
RA Ramakrishnan K., Okkema P.G.;
RT "Regulation of C. elegans neuronal differentiation by the ZEB-family factor
RT ZAG-1 and the NK-2 homeodomain factor CEH-28.";
RL PLoS ONE 9:e113893-e113893(2014).
CC -!- FUNCTION: Probable transcription factor that regulates neuronal
CC differention, including synapse assembly of the cholinergic motor
CC neuron M4 (PubMed:24690231, PubMed:25474681). Activates expression of
CC growth factor, neuropeptide and transcription factor genes, such as
CC TGF-beta dbl-1, FMRFamide-like flp-5 and transcription repressor zag-1,
CC in the M4 neuron (PubMed:24690231, PubMed:25474681). Required for
CC pharynx peristalsis (PubMed:18161854). {ECO:0000269|PubMed:18161854,
CC ECO:0000269|PubMed:24690231, ECO:0000269|PubMed:25474681}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000255|RuleBase:RU000682}.
CC -!- DEVELOPMENTAL STAGE: Expressed from 172 minutes after the first
CC cleavage, in the ABalaappa cell and continuing in its daughters, and in
CC the cholinergic motor neuron M4 from mid-embryogenesis through to adult
CC stages. {ECO:0000269|PubMed:18161854}.
CC -!- DISRUPTION PHENOTYPE: Reduced pharynx pumping rate and abnormal isthmus
CC peristalses in L1 stage larvae (PubMed:18161854). Feeding defects,
CC characterized by a stuffed pharynx, as a result of ingested E.coli
CC packing the lumen of the corpus and anterior isthmus in L4 stage larvae
CC (PubMed:18161854). Defects in pharyngeal g1 gland cell morphology
CC (PubMed:24690231). Reduced expression of TGF-beta, dbl-1, in the
CC cholinergic motor neuron M4, but normal expression of other markers of
CC differentiation, such as serotonin receptor ser-7b and the vesicular
CC acetylcholine transporter unc-17 (PubMed:18161854).
CC {ECO:0000269|PubMed:18161854, ECO:0000269|PubMed:24690231}.
CC -!- SIMILARITY: Belongs to the NK-2 homeobox family. {ECO:0000305}.
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DR EMBL; AY205144; AAO46042.2; -; mRNA.
DR EMBL; BX284606; CAB00867.4; -; Genomic_DNA.
DR RefSeq; NP_510169.4; NM_077768.6.
DR AlphaFoldDB; G5EE18; -.
DR SMR; G5EE18; -.
DR IntAct; G5EE18; 3.
DR STRING; 6239.K03A11.3; -.
DR PaxDb; G5EE18; -.
DR EnsemblMetazoa; K03A11.3.1; K03A11.3.1; WBGene00000450.
DR GeneID; 191619; -.
DR KEGG; cel:CELE_K03A11.3; -.
DR CTD; 191619; -.
DR WormBase; K03A11.3; CE42603; WBGene00000450; ceh-28.
DR eggNOG; KOG0842; Eukaryota.
DR GeneTree; ENSGT00940000168126; -.
DR HOGENOM; CLU_1361488_0_0_1; -.
DR InParanoid; G5EE18; -.
DR OMA; PYQPVIP; -.
DR OrthoDB; 1380390at2759; -.
DR PhylomeDB; G5EE18; -.
DR Proteomes; UP000001940; Chromosome X.
DR GO; GO:0000785; C:chromatin; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:WormBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:WormBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IMP:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0045664; P:regulation of neuron differentiation; IMP:UniProtKB.
DR GO; GO:0050807; P:regulation of synapse organization; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0034243; P:regulation of transcription elongation from RNA polymerase II promoter; ISS:WormBase.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR000047; HTH_motif.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Homeobox; Neurogenesis; Nucleus; Reference proteome.
FT CHAIN 1..201
FT /note="Homeobox protein ceh-28"
FT /id="PRO_0000452001"
FT DNA_BIND 104..163
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 72..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 201 AA; 22995 MW; 7B8B3413D1D19091 CRC64;
MQSTQISSTT VILPSEVIQP PQQSAVPSEF KNTLPSRLNL FEGFDQSFSE LTNPYQPVIP
LMQRESLLGA SSYYSSPSQN QRSYQNHRQH SNPDTINLRS QQQKRKPRVL FTQHQVNELE
ERFKKQRYVT ATEREELAQC LGLTATQVKI WFQNRRYKCK RLAQDRTLQL SQIPFNPMFA
SAFPFGINSF GTAPSSSSSG S
