ID   CEIB_ECOLX              Reviewed;         626 AA.
AC   P04479;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Colicin-Ib;
GN   Name=cib;
OS   Escherichia coli.
OG   Plasmid IncI1 ColIb-P9.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3531169; DOI=10.1128/jb.168.1.228-236.1986;
RA   Mankovich J.A., Hsu C.-H., Konisky J.;
RT   "DNA and amino acid sequence analysis of structural and immunity genes of
RT   colicins Ia and Ib.";
RL   J. Bacteriol. 168:228-236(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
RX   PubMed=6204975; DOI=10.1016/s0021-9258(17)47219-x;
RA   Mankovich J.A., Lai P.H., Gokul N., Konisky J.;
RT   "Organization of the colicin Ib gene. Promoter structure and immunity
RT   domain.";
RL   J. Biol. Chem. 259:8764-8768(1984).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6091036; DOI=10.1093/nar/12.17.6727;
RA   Varley J.M., Boulnois G.J.;
RT   "Analysis of a cloned colicin Ib gene: complete nucleotide sequence and
RT   implications for regulation of expression.";
RL   Nucleic Acids Res. 12:6727-6739(1984).
RN   [4]
RP   ERRATUM OF PUBMED:6091036.
RA   Varley J.M., Boulnois G.J.;
RL   Nucleic Acids Res. 12:8748-8748(1984).
CC   -!- FUNCTION: This colicin is a channel-forming colicin. This class of
CC       transmembrane toxins depolarize the cytoplasmic membrane, leading to
CC       dissipation of cellular energy.
CC   -!- FUNCTION: Colicins are polypeptide toxins produced by and active
CC       against E.coli and closely related bacteria.
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the channel forming colicin family.
CC       {ECO:0000305}.
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DR   EMBL; K02071; AAA92225.1; -; Genomic_DNA.
DR   EMBL; X01009; CAA25505.1; -; Genomic_DNA.
DR   EMBL; M13820; AAA23188.1; -; Genomic_DNA.
DR   PIR; A93533; IKECB.
DR   AlphaFoldDB; P04479; -.
DR   SMR; P04479; -.
DR   TCDB; 1.C.1.1.2; the channel-forming colicin (colicin) family.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IEA:InterPro.
DR   Gene3D; 1.10.490.30; -; 1.
DR   InterPro; IPR000293; Channel_colicin_C.
DR   InterPro; IPR038283; Channel_colicin_C_sf.
DR   InterPro; IPR014740; Channel_colicin_cen.
DR   InterPro; IPR014739; Channel_colicin_N_sf.
DR   Pfam; PF01024; Colicin; 1.
DR   Pfam; PF11504; Colicin_Ia; 1.
DR   PRINTS; PR00280; CHANLCOLICIN.
DR   SUPFAM; SSF58096; SSF58096; 1.
DR   PROSITE; PS00276; CHANNEL_COLICIN; 1.
PE   3: Inferred from homology;
KW   Antibiotic; Antimicrobial; Bacteriocin; Host membrane; Membrane; Plasmid;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..626
FT                   /note="Colicin-Ib"
FT                   /id="PRO_0000218676"
FT   TRANSMEM        588..612
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          276..308
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   626 AA;  69924 MW;  1BE67D6F7C08A528 CRC64;
     MSDPVRITNP GAESLGYDSD GHEIMAVDIY VNPPRVDVFH GTPPAWSSFG NKTIWGGNEW
     VDDSPTRSDI EKRDKEITAY KNTLSAQQKE NENKRTEAGK RLSAAIAARE KDENTLKTLR
     AGNADAADIT RQEFRLLQAE LREYGFRTEI AGYDALRLHT ESRMLFADAD SLRISPREAR
     SLIEQAEKRQ KDAQNADKKA ADMLAEYERR KGILDTRLSE LEKNGGAALA VLDAQQARLL
     GQQTRNDRAI SEARNKLSSV TESLKTARNA LTRAEQQLTQ QKNTPDGKTI VSPEKFPGRS
     STNHSIVVSG DPRFAGTIKI TTSAVIDNRA NLNYLLTHSG LDYKRNILND RNPVVTEDVE
     GDKKIYNAEV AEWDKLRQRL LDARNKITSA ESAINSARNN VSARTNEQKH ANDALNALLK
     EKENIRSQLA DINQKIAEEK RKRDEINMVK DAIKLTSDFY RTIYDEFGKQ ASELAKELAS
     VSQGKQIKSV DDALNAFDKF RNNLNKKYNI QDRMAISKAL EAINQVHMAE NFKLFSKAFG
     FTGKVIERYD VAVELQKAVK TDNWRPFFVK LESLAAGRAA SAVTAWAFSV MLGTPVGILG
     FAIIMAAVSA LVNDKFIEQV NKLIGI