CEL1B_XENLA
ID CEL1B_XENLA Reviewed; 489 AA.
AC Q6PF35; Q6NRN9;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=CUGBP Elav-like family member 1-B;
DE Short=CELF-1B;
DE AltName: Full=Bruno-like protein 2-B;
DE AltName: Full=CUG triplet repeat RNA-binding protein 1-B;
DE Short=CUG-BP1-B;
DE AltName: Full=CUG-BP- and ETR-3-like factor 1-B;
DE AltName: Full=Embryo deadenylation element-binding protein B;
DE Short=EDEN-BP-B;
DE AltName: Full=RNA-binding protein BRUNOL-2-B;
DE AltName: Full=p53/p55;
GN Name=cugbp1-b; Synonyms=celf1-b;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Spleen;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: RNA-binding protein implicated in the regulation of several
CC post-transcriptional events. May be involved in pre-mRNA alternative
CC splicing, mRNA translation activation and stability (By similarity).
CC Mediates the rapid and sequence-specific cytoplasmic deadenylation of
CC EDEN-containing maternal mRNAs following fertilization. Binds to AU-
CC rich sequences (AREs) of jun mRNA. Binds to the embryonic deadenylation
CC element (EDEN) motif localized in the 3'-UTR of maternal mRNAs. Binds
CC to RNA containing several repeats of the consensus sequence 5'-UGU-3'.
CC EDEN-dependent deadenylation is enhanced by the presence of an
CC additional cis element composed of three AUU repeats (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Oligomer. Oligomerization is required for RNA-binding and
CC EDEN-dependent deadenylation. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6PF35-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6PF35-2; Sequence=VSP_026794, VSP_026795;
CC -!- DOMAIN: The 2 N-terminal RRMs and a part of the linker region (between
CC RRM2 and RRM3) are necessary for binding to EDEN of mos mRNA.
CC {ECO:0000250}.
CC -!- PTM: Phosphorylated during oocyte maturation and dephosphorylated
CC following egg activation. Dephosphorylation is calcium dependent and
CC correlates with the increase in the activity of EDEN-dependent
CC deadenylation (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CELF/BRUNOL family. {ECO:0000305}.
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DR EMBL; BC057743; AAH57743.1; -; mRNA.
DR EMBL; BC070706; AAH70706.1; -; mRNA.
DR RefSeq; NP_001079970.1; NM_001086501.1.
DR AlphaFoldDB; Q6PF35; -.
DR SMR; Q6PF35; -.
DR DNASU; 379661; -.
DR GeneID; 379661; -.
DR KEGG; xla:379661; -.
DR CTD; 379661; -.
DR Xenbase; XB-GENE-6255183; celf1.S.
DR OrthoDB; 1209165at2759; -.
DR Proteomes; UP000186698; Chromosome 4S.
DR Bgee; 379661; Expressed in neurula embryo and 19 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:1990904; C:ribonucleoprotein complex; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR CDD; cd12631; RRM1_CELF1_2_Bruno; 1.
DR CDD; cd12634; RRM2_CELF1_2; 1.
DR CDD; cd12638; RRM3_CELF1_2; 1.
DR Gene3D; 3.30.70.330; -; 3.
DR InterPro; IPR034196; CELF1/2_RRM1.
DR InterPro; IPR034198; CELF1/2_RRM2.
DR InterPro; IPR034199; CELF1/2_RRM3.
DR InterPro; IPR002343; Hud_Sxl_RNA.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 3.
DR PRINTS; PR00961; HUDSXLRNA.
DR SMART; SM00360; RRM; 3.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50102; RRM; 3.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; Cytoplasm; mRNA processing; mRNA splicing;
KW Nucleus; Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..489
FT /note="CUGBP Elav-like family member 1-B"
FT /id="PRO_0000295187"
FT DOMAIN 16..99
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 108..188
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 404..482
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT VAR_SEQ 1
FT /note="M -> MASFKLDFLPEMMVDHCSLNSSPVSKKM (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_026794"
FT VAR_SEQ 104
FT /note="A -> GGLNTVLFPEHPAS (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_026795"
FT CONFLICT 290
FT /note="A -> T (in Ref. 1; AAH70706)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 489 AA; 52445 MW; D43830EA88C1B079 CRC64;
MNGTMDHPDH PDSDSIKMFV GQVPRSWSEK ELRELFEQYG AVYEINVLRD RSQNPPQSKG
CCFITFYTRK AALEAQNALH NMKVLPGMHH PIQMKPADSE KNNAVEDRKL FIGMVSKKCN
ENDIRTLFSQ FGQIEESRIL RGPDGMSRGC AFITFTTRSM AQMAIKAMHQ AQTMEGCSSP
IVVKFADTQK DKEQKRMTQQ LQQQMQQLNA ASMWGNLAGL GSLAPQYLAL LQQTTSSGNL
NSLSGLHPMG GEYATGMTSG LNAMQLQNLA TLAAAASAAQ NTQSAGSALA SSSSPLSILT
SSGSSPNSNN SSINHMASLG ALQTLAGATA GLNVGSLAGM AALNGGLGSS LSNGTGSTME
ALSQAYSGIQ QYAAAALPSL YNQSLLSQQG LGAAGSQKEG PEGANLFIYH LPQEFGDQDL
LQMFMPFGNI VSAKVFIDKQ TNLSKCFGFI SYDNPVSAQA AIQSMNGFQI GMKRLKVQLK
RSKNDSKPY
