CELA1_FELCA
ID CELA1_FELCA Reviewed; 266 AA.
AC Q5R1M5;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Chymotrypsin-like elastase family member 1;
DE EC=3.4.21.36;
DE AltName: Full=Elastase-1;
DE Flags: Precursor;
GN Name=CELA1; Synonyms=ELA1, ELS1;
OS Felis catus (Cat) (Felis silvestris catus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Feliformia; Felidae; Felinae; Felis.
OX NCBI_TaxID=9685;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Pancreas;
RA Sato N., Kano R., Hasegawa A.;
RT "Felis catus ELS1 mRNA for elastase 1, complete cds.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Acts upon elastin. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of proteins, including elastin. Preferential
CC cleavage: Ala-|-Xaa.; EC=3.4.21.36;
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the peptidase S1 family. Elastase subfamily.
CC {ECO:0000255|PROSITE-ProRule:PRU00274}.
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DR EMBL; AB196342; BAD77813.1; -; mRNA.
DR RefSeq; NP_001009389.1; NM_001009389.2.
DR AlphaFoldDB; Q5R1M5; -.
DR SMR; Q5R1M5; -.
DR STRING; 9685.ENSFCAP00000004073; -.
DR MEROPS; S01.153; -.
DR GeneID; 494217; -.
DR KEGG; fca:494217; -.
DR CTD; 1990; -.
DR eggNOG; KOG3627; Eukaryota.
DR InParanoid; Q5R1M5; -.
DR OrthoDB; 1314811at2759; -.
DR TreeFam; TF330455; -.
DR Proteomes; UP000011712; Unplaced.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR CDD; cd00190; Tryp_SPc; 1.
DR Gene3D; 2.40.10.10; -; 2.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001314; Peptidase_S1A.
DR InterPro; IPR001254; Trypsin_dom.
DR InterPro; IPR018114; TRYPSIN_HIS.
DR InterPro; IPR033116; TRYPSIN_SER.
DR Pfam; PF00089; Trypsin; 1.
DR PRINTS; PR00722; CHYMOTRYPSIN.
DR SMART; SM00020; Tryp_SPc; 1.
DR SUPFAM; SSF50494; SSF50494; 1.
DR PROSITE; PS50240; TRYPSIN_DOM; 1.
DR PROSITE; PS00134; TRYPSIN_HIS; 1.
DR PROSITE; PS00135; TRYPSIN_SER; 1.
PE 2: Evidence at transcript level;
KW Calcium; Disulfide bond; Glycoprotein; Hydrolase; Metal-binding; Protease;
KW Reference proteome; Secreted; Serine protease; Signal; Zymogen.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT PROPEP 17..26
FT /note="Activation peptide"
FT /id="PRO_0000027675"
FT CHAIN 27..266
FT /note="Chymotrypsin-like elastase family member 1"
FT /id="PRO_0000027676"
FT DOMAIN 27..264
FT /note="Peptidase S1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT ACT_SITE 71
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 119
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 214
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT BINDING 85
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 90
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 95
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT CARBOHYD 87
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 241
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 56..72
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 153..220
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 184..200
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 210..240
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
SQ SEQUENCE 266 AA; 28841 MW; 5CF022A502C03AB2 CRC64;
MLRFLVLATL VLYGHSTRDF PETNARVVGG TEARKNPWPS QISLQYLSGG KWYHTCGGTL
IRQNWVMTAA HCVDRKMTFR VVAGEHNLSQ NDGTEQRVSV QKIVVHPYWN SNNVAAGYDI
ALLRLAQRVT LNNYVQLGVL PAAGTILANN NPCYITGWGM TKTNGQLAQA LQQAYLPSVD
YATCSSSSYW GSTVKSTMVC AGGDGIRSGC QGDSGGPLHC LVNGKYAVHG VTSFVSSLGC
NVSRKPTVFT RVSAYISWIN NVIASN