CELB_LACLA
ID CELB_LACLA Reviewed; 494 AA.
AC Q9CJ32;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 102.
DE RecName: Full=PTS system cellobiose-specific EIIC component {ECO:0000305};
GN Name=celB {ECO:0000303|PubMed:17909747};
GN ORFNames=L177520 {ECO:0000312|EMBL:AAK04272.1};
OS Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
OX NCBI_TaxID=272623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IL1403;
RX PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA Ehrlich S.D., Sorokin A.;
RT "The complete genome sequence of the lactic acid bacterium Lactococcus
RT lactis ssp. lactis IL1403.";
RL Genome Res. 11:731-753(2001).
RN [2]
RP FUNCTION, AND INDUCTION.
RC STRAIN=IL1403;
RX PubMed=17909747; DOI=10.1007/s00203-007-0308-8;
RA Kowalczyk M., Cocaign-Bousquet M., Loubiere P., Bardowski J.;
RT "Identification and functional characterisation of cellobiose and lactose
RT transport systems in Lactococcus lactis IL1403.";
RL Arch. Microbiol. 189:187-196(2008).
RN [3]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=IL1403;
RX PubMed=21262549; DOI=10.1016/j.ijfoodmicro.2010.12.011;
RA Aleksandrzak-Piekarczyk T., Polak J., Jezierska B., Renault P.,
RA Bardowski J.;
RT "Genetic characterization of the CcpA-dependent, cellobiose-specific PTS
RT system comprising CelB, PtcB and PtcA that transports lactose in
RT Lactococcus lactis IL1403.";
RL Int. J. Food Microbiol. 145:186-194(2011).
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC system (PTS), a major carbohydrate active transport system, catalyzes
CC the phosphorylation of incoming sugar substrates concomitant with their
CC translocation across the cell membrane (By similarity). Involved in
CC cellobiose transport with PtcA and PtcB. This system can also transport
CC lactose (PubMed:17909747, PubMed:21262549).
CC {ECO:0000250|UniProtKB:P17334, ECO:0000269|PubMed:17909747,
CC ECO:0000269|PubMed:21262549}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00428}; Multi-pass membrane protein {ECO:0000255|PROSITE-
CC ProRule:PRU00428}.
CC -!- INDUCTION: Induced by growth on cellobiose. Negativelly controled by
CC the CcpA regulator. {ECO:0000269|PubMed:17909747,
CC ECO:0000269|PubMed:21262549}.
CC -!- DOMAIN: The EIIC type-3 domain forms the PTS system translocation
CC channel and contains the specific substrate-binding site.
CC {ECO:0000255|PROSITE-ProRule:PRU00428}.
CC -!- DISRUPTION PHENOTYPE: Disruption of the gene abolishes growth on
CC cellobiose and lactose. {ECO:0000269|PubMed:21262549}.
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DR EMBL; AE005176; AAK04272.1; -; Genomic_DNA.
DR PIR; F86646; F86646.
DR RefSeq; NP_266330.1; NC_002662.1.
DR RefSeq; WP_010905173.1; NC_002662.1.
DR AlphaFoldDB; Q9CJ32; -.
DR SMR; Q9CJ32; -.
DR STRING; 272623.L177520; -.
DR TCDB; 4.A.3.2.4; the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family.
DR PaxDb; Q9CJ32; -.
DR EnsemblBacteria; AAK04272; AAK04272; L177520.
DR KEGG; lla:L177520; -.
DR PATRIC; fig|272623.7.peg.194; -.
DR eggNOG; COG1455; Bacteria.
DR HOGENOM; CLU_029688_1_2_9; -.
DR OMA; QLFWVIG; -.
DR Proteomes; UP000002196; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR InterPro; IPR003352; PTS_EIIC.
DR InterPro; IPR004501; PTS_EIIC_3.
DR Pfam; PF02378; PTS_EIIC; 1.
DR TIGRFAMs; TIGR00410; lacE; 1.
DR PROSITE; PS51105; PTS_EIIC_TYPE_3; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Phosphotransferase system; Reference proteome;
KW Sugar transport; Transferase; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..494
FT /note="PTS system cellobiose-specific EIIC component"
FT /id="PRO_0000446882"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT TRANSMEM 119..139
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT TRANSMEM 188..208
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT TRANSMEM 355..375
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT TRANSMEM 406..426
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT TRANSMEM 463..483
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT DOMAIN 8..481
FT /note="PTS EIIC type-3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
SQ SEQUENCE 494 AA; 52780 MW; 63DCB0803D9B7868 CRC64;
MNGITAWMEK YLVPVAAKIG SQKHLVALRD SFIGMLPATL AGALAAMISA IVTTFPSAIQ
QMMLGATAFS KLAPEKVWTL ANTPIIGDLN NISALVNQGT LTVIGLIFAF SWGYNLARAY
GVNDLAGGIV SLATLFAGLP NQMGKFTAAL GTGKAGVAAT DKLNGVLGDQ GLAAWKPLFA
SAHLDAGAYF TVIIMGALAV IIYAKLMLAD ITIKMPESVP PAVAKAFLAI IPTIAALYIV
GLIYYIIGKL TNDSVINLIT HYIAEPFQIL SQNIFSVLIV TLFVSVFWFF GLHGPNVLAP
VLDGIWGPLG LNNQALYFQV HSQGIRDLIA KGAVDKAHAI NGDYVNLWVR GSWDAFAWFG
GSGGTITLVI AIILFSKRKD YKIVGRLGLA PGIFNINEPV LFGLPVVLNA IFFIPFAVAP
LISVIIAYTA TALHLVDPVV NAVPWVTPPI MNAFMATGFD WRAIVLTIIN LIITFVIWVP
FVIAANKLEE TELD
