CELF1_DANRE
ID CELF1_DANRE Reviewed; 501 AA.
AC Q9IBD0; Q7ZWE4;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=CUGBP Elav-like family member 1;
DE Short=CELF-1;
DE AltName: Full=Bruno-like protein 2;
DE AltName: Full=CUG triplet repeat RNA-binding protein 1;
DE Short=CUG-BP1;
DE AltName: Full=CUG-BP- and ETR-3-like factor 1;
DE AltName: Full=EDEN-BP/Bruno-like protein;
DE AltName: Full=RNA-binding protein BRUNOL-2;
GN Name=celf1; Synonyms=brul, cugbp1;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND DEVELOPMENTAL STAGE.
RX PubMed=10781958; DOI=10.1016/s0925-4773(00)00270-7;
RA Suzuki H., Maegawa S., Nishibu T., Sugiyama T., Yasuda K., Inoue K.;
RT "Vegetal localization of the maternal mRNA encoding an EDEN-BP/Bruno-like
RT protein in zebrafish.";
RL Mech. Dev. 93:205-209(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, AND RNA-BINDING.
RX PubMed=11895477; DOI=10.1046/j.1356-9597.2001.00506.x;
RA Suzuki H., Jin Y., Otani H., Yasuda K., Inoue K.;
RT "Regulation of alternative splicing of alpha-actinin transcript by Bruno-
RT like proteins.";
RL Genes Cells 7:133-141(2002).
CC -!- FUNCTION: RNA-binding protein implicated in the regulation of several
CC post-transcriptional events. May be involved in mRNA translation
CC activation and stability (By similarity). Involved in the regulation of
CC muscle-specific splicing of alpha actinin pre-mRNAs via the binding to
CC the UR-repeat element (URE) at the branch point of the non-muscle (NM)
CC exon. {ECO:0000250, ECO:0000269|PubMed:11895477}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9IBD0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9IBD0-2; Sequence=VSP_026791, VSP_026792, VSP_026793;
CC -!- DEVELOPMENTAL STAGE: Expressed in the vegetal cortex of the oocyte.
CC Expressed uniformly in the embryo. Expressed in the vegetal pole at
CC 0.08 hour post-fertilization (hpf). Expressed in the lens fiber cells
CC at 24 hpf and in the blastodisc of the zygote at 30 hpf.
CC {ECO:0000269|PubMed:10781958}.
CC -!- SIMILARITY: Belongs to the CELF/BRUNOL family. {ECO:0000305}.
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DR EMBL; AB032726; BAA95119.1; -; mRNA.
DR EMBL; BC049453; AAH49453.1; -; mRNA.
DR RefSeq; NP_001315342.1; NM_001328413.1. [Q9IBD0-2]
DR RefSeq; NP_571688.1; NM_131613.2. [Q9IBD0-1]
DR RefSeq; XP_017212394.1; XM_017356905.1.
DR AlphaFoldDB; Q9IBD0; -.
DR SMR; Q9IBD0; -.
DR STRING; 7955.ENSDARP00000111278; -.
DR PaxDb; Q9IBD0; -.
DR PRIDE; Q9IBD0; -.
DR Ensembl; ENSDART00000041055; ENSDARP00000041054; ENSDARG00000005315. [Q9IBD0-2]
DR Ensembl; ENSDART00000134570; ENSDARP00000115192; ENSDARG00000005315. [Q9IBD0-1]
DR GeneID; 58137; -.
DR KEGG; dre:58137; -.
DR CTD; 10658; -.
DR ZFIN; ZDB-GENE-000501-1; celf1.
DR eggNOG; KOG0144; Eukaryota.
DR GeneTree; ENSGT00940000158970; -.
DR HOGENOM; CLU_015367_0_0_1; -.
DR InParanoid; Q9IBD0; -.
DR OMA; QWHKPRK; -.
DR OrthoDB; 1209165at2759; -.
DR PhylomeDB; Q9IBD0; -.
DR PRO; PR:Q9IBD0; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 7.
DR Bgee; ENSDARG00000005315; Expressed in mature ovarian follicle and 39 other tissues.
DR ExpressionAtlas; Q9IBD0; baseline.
DR GO; GO:0005737; C:cytoplasm; IDA:ZFIN.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0045495; C:pole plasm; IDA:ZFIN.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IDA:ZFIN.
DR GO; GO:0003729; F:mRNA binding; IDA:ZFIN.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0016477; P:cell migration; IMP:CACAO.
DR GO; GO:0007368; P:determination of left/right symmetry; IMP:ZFIN.
DR GO; GO:0048588; P:developmental cell growth; IMP:CACAO.
DR GO; GO:0048565; P:digestive tract development; IMP:ZFIN.
DR GO; GO:1990402; P:embryonic liver development; IMP:ZFIN.
DR GO; GO:0061031; P:endodermal digestive tract morphogenesis; IMP:ZFIN.
DR GO; GO:0070306; P:lens fiber cell differentiation; IMP:ZFIN.
DR GO; GO:0001889; P:liver development; IMP:CACAO.
DR GO; GO:0006376; P:mRNA splice site selection; IBA:GO_Central.
DR GO; GO:0031016; P:pancreas development; IMP:ZFIN.
DR GO; GO:0061113; P:pancreas morphogenesis; IMP:CACAO.
DR GO; GO:0061014; P:positive regulation of mRNA catabolic process; IMP:ZFIN.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IBA:GO_Central.
DR CDD; cd12631; RRM1_CELF1_2_Bruno; 1.
DR CDD; cd12634; RRM2_CELF1_2; 1.
DR CDD; cd12638; RRM3_CELF1_2; 1.
DR Gene3D; 3.30.70.330; -; 3.
DR InterPro; IPR034196; CELF1/2_RRM1.
DR InterPro; IPR034198; CELF1/2_RRM2.
DR InterPro; IPR034199; CELF1/2_RRM3.
DR InterPro; IPR002343; Hud_Sxl_RNA.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 3.
DR PRINTS; PR00961; HUDSXLRNA.
DR SMART; SM00360; RRM; 3.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50102; RRM; 3.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Cytoplasm; mRNA processing; mRNA splicing;
KW Nucleus; Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..501
FT /note="CUGBP Elav-like family member 1"
FT /id="PRO_0000295185"
FT DOMAIN 16..99
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 108..188
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 416..494
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 2..196
FT /note="Binds strongly to URE"
FT REGION 274..323
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 397..501
FT /note="Binds strongly to URE"
FT VAR_SEQ 1
FT /note="M -> MDSIEAEALYLTTAQHGQPQCELSLPAVEVPALGGSKKM (in
FT isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_026791"
FT VAR_SEQ 149
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_026792"
FT VAR_SEQ 230..233
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_026793"
SQ SEQUENCE 501 AA; 53608 MW; A1391FF0F7A839B2 CRC64;
MNGSLDHPDQ PDIDSIKMFV GQIPRTWSED QLRELFEPYG AVYEINVLRD RSQNPPQSKG
CCFVTYYTRK SALEAQNALH NMKILPGMHH PIQMKPADSE KNNAVEDRKL FVGMISKKCN
ENDIRLMFSP YGQIEECRIL RGPDGLSRGC AFVTFTARQM AQSAIKSMHQ SQTMEGCSSP
IVVKFADTQK DKEQKRIAQQ LQQQMQQLNA ASMWGNLTGL NSLGPQYLAL YLQLLQQSAS
SGNALNNLHP MSGLNAMQNL AALAAAASAT QATPTGSSAL TTSSSPLSVL TSSGTPSGQP
AQSAWDAYKA GSSPTSSTSS SVNPMASLGA LQSLAAGAGA GLNMSSLASM AALNGGLGSG
GLSNGSGSTM EALTQAAYSG IQQYAAAALP SLYSQSLLSQ QNVSAAGSQK EGPEGANLFI
YHLPQEFGDQ DLLQMFMPFG NVISAKVFID KQTNLSKCFG FVSYDNPVSS QAAIQSMNGF
QIGMKRLKVQ LKRSKNDSKP Y
