ID   CELF1_XENTR             Reviewed;         490 AA.
AC   Q28HE9;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=CUGBP Elav-like family member 1;
DE            Short=CELF-1;
DE   AltName: Full=Bruno-like protein 2;
DE   AltName: Full=CUG triplet repeat RNA-binding protein 1;
DE            Short=CUG-BP1;
DE   AltName: Full=CUG-BP- and ETR-3-like factor 1;
DE   AltName: Full=RNA-binding protein BRUNOL-2;
GN   Name=celf1; Synonyms=cugbp1; ORFNames=TEgg064f02.1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA-binding protein implicated in the regulation of several
CC       post-transcriptional events. May be involved in pre-mRNA alternative
CC       splicing, mRNA translation activation and stability (By similarity).
CC       Mediates the rapid and sequence-specific cytoplasmic deadenylation of
CC       EDEN-containing maternal mRNAs following fertilization. Binds to AU-
CC       rich sequences (AREs) of jun mRNA. Binds to the embryonic deadenylation
CC       element (EDEN) motif localized in the 3'-UTR of maternal mRNAs. Binds
CC       to RNA containing several repeats of the consensus sequence 5'-UGU-3'.
CC       EDEN-dependent deadenylation is enhanced by the presence of an
CC       additional cis element composed of three AUU repeats (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Oligomer. Oligomerization is required for RNA-binding and
CC       EDEN-dependent deadenylation. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC   -!- DOMAIN: The 2 N-terminal RRMs and a part of the linker region (between
CC       RRM2 and RRM3) are necessary for binding to EDEN of mos mRNA.
CC       {ECO:0000250}.
CC   -!- PTM: Phosphorylated during oocyte maturation and dephosphorylated
CC       following egg activation. Dephosphorylation is calcium dependent and
CC       correlates with the increase in the activity of EDEN-dependent
CC       deadenylation (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CELF/BRUNOL family. {ECO:0000305}.
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DR   EMBL; CR760913; CAJ82289.1; -; mRNA.
DR   RefSeq; NP_001017152.1; NM_001017152.2.
DR   AlphaFoldDB; Q28HE9; -.
DR   BMRB; Q28HE9; -.
DR   SMR; Q28HE9; -.
DR   STRING; 8364.ENSXETP00000019816; -.
DR   PaxDb; Q28HE9; -.
DR   GeneID; 549906; -.
DR   KEGG; xtr:549906; -.
DR   CTD; 10658; -.
DR   Xenbase; XB-GENE-854036; celf1.
DR   eggNOG; KOG0144; Eukaryota.
DR   HOGENOM; CLU_015367_0_2_1; -.
DR   InParanoid; Q28HE9; -.
DR   OrthoDB; 1209165at2759; -.
DR   Proteomes; UP000008143; Chromosome 4.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR   GO; GO:0003730; F:mRNA 3'-UTR binding; IBA:GO_Central.
DR   GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0006376; P:mRNA splice site selection; IBA:GO_Central.
DR   GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IBA:GO_Central.
DR   CDD; cd12631; RRM1_CELF1_2_Bruno; 1.
DR   CDD; cd12634; RRM2_CELF1_2; 1.
DR   CDD; cd12638; RRM3_CELF1_2; 1.
DR   Gene3D; 3.30.70.330; -; 3.
DR   InterPro; IPR034196; CELF1/2_RRM1.
DR   InterPro; IPR034198; CELF1/2_RRM2.
DR   InterPro; IPR034199; CELF1/2_RRM3.
DR   InterPro; IPR002343; Hud_Sxl_RNA.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   Pfam; PF00076; RRM_1; 3.
DR   PRINTS; PR00961; HUDSXLRNA.
DR   SMART; SM00360; RRM; 3.
DR   SUPFAM; SSF54928; SSF54928; 2.
DR   PROSITE; PS50102; RRM; 3.
PE   2: Evidence at transcript level;
KW   Activator; Cytoplasm; mRNA processing; mRNA splicing; Nucleus;
KW   Reference proteome; Repeat; RNA-binding.
FT   CHAIN           1..490
FT                   /note="CUGBP Elav-like family member 1"
FT                   /id="PRO_0000295188"
FT   DOMAIN          16..99
FT                   /note="RRM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          108..188
FT                   /note="RRM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          405..483
FT                   /note="RRM 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   REGION          283..312
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   490 AA;  52387 MW;  73AEEB55E18C5382 CRC64;
     MNGTMDHPDH PDPDSIKMFV GQVPRSWSEK ELRELFEQYG AVYEINVLRD RSQNPPQSKG
     CCFITFYTRK AALEAQNALH NMKVLPGMHH PIQMKPADSE KNNAVEDRKL FVGMVSKKCN
     ENDIRAMFSQ FGQIEESRIL RGPDGMSRGC AFVTFTTRSM AQMAIKAMHQ AQTMEGCSSP
     IVVKFADTQK DKEQKRMTQQ LQQQMQQLNA ASMWGNLAGL SSLAPQYLAL LQQTASSGNL
     NSLSGLHPMG GEYATGMTSG LNAMQLQNLA ALAAAASAAQ NTPSAGSALT TSSSPLSILT
     SSGSSPSSNN NSAVNPMASL GALQTLAGAT AGLNVGSLAG MAALNGGLGS SLSNGTGSTM
     EALSQAYSGI QQYAAAALPS LYNQSLLSQQ GLGAAGSQKE GPEGANLFIY HLPQEFGDQD
     LLQMFMPFGN VVSAKVFIDK QTNLSKCFGF VSYDNPVSAQ AAIQSMNGFQ IGMKRLKVQL
     KRSKNDSKPY