CELF2_CHICK
ID CELF2_CHICK Reviewed; 484 AA.
AC Q7T2T1; Q7T2T0;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=CUGBP Elav-like family member 2;
DE Short=CELF-2;
DE AltName: Full=Bruno-like protein 3;
DE AltName: Full=CUG triplet repeat RNA-binding protein 2;
DE Short=CUG-BP2;
DE AltName: Full=CUG-BP- and ETR-3-like factor 2;
DE AltName: Full=ELAV-type RNA-binding protein 3;
DE Short=ETR-3;
DE AltName: Full=RNA-binding protein BRUNOL-3;
GN Name=CELF2; Synonyms=CUGBP2;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Heart;
RX PubMed=15226369; DOI=10.1242/jcs.01194;
RA Ladd A.N., Cooper T.A.;
RT "Multiple domains control the subcellular localization and activity of ETR-
RT 3, a regulator of nuclear and cytoplasmic RNA processing events.";
RL J. Cell Sci. 117:3519-3529(2004).
RN [2]
RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=15830352; DOI=10.1002/dvdy.20382;
RA Ladd A.N., Stenberg M.G., Swanson M.S., Cooper T.A.;
RT "Dynamic balance between activation and repression regulates pre-mRNA
RT alternative splicing during heart development.";
RL Dev. Dyn. 233:783-793(2005).
CC -!- FUNCTION: RNA-binding protein implicated in the regulation of several
CC post-transcriptional events. May be involved in mRNA translation
CC repression and stability. Mediates exon inclusion in TNNT2 pre-mRNA.
CC {ECO:0000269|PubMed:15226369}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15830352}. Cytoplasm
CC {ECO:0000269|PubMed:15830352}. Note=Shuttles between the nucleus and
CC the cytoplasm in a CRM1-dependent manner.
CC {ECO:0000269|PubMed:15830352}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=Variant L;
CC IsoId=Q7T2T1-1; Sequence=Displayed;
CC Name=2; Synonyms=Variant 4;
CC IsoId=Q7T2T1-2; Sequence=VSP_026816;
CC -!- TISSUE SPECIFICITY: Expressed in heart. {ECO:0000269|PubMed:15226369}.
CC -!- DEVELOPMENTAL STAGE: Expressed in heart at embryonic day 12 and 14 (at
CC protein level). Expressed in heart at embryonic day 8.
CC {ECO:0000269|PubMed:15226369, ECO:0000269|PubMed:15830352}.
CC -!- SIMILARITY: Belongs to the CELF/BRUNOL family. {ECO:0000305}.
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DR EMBL; AY288985; AAP57761.1; -; mRNA.
DR EMBL; AY288986; AAP57762.1; -; mRNA.
DR RefSeq; NP_989591.2; NM_204260.2.
DR AlphaFoldDB; Q7T2T1; -.
DR BMRB; Q7T2T1; -.
DR SMR; Q7T2T1; -.
DR BioGRID; 675146; 1.
DR STRING; 9031.ENSGALP00000010845; -.
DR PaxDb; Q7T2T1; -.
DR GeneID; 374111; -.
DR KEGG; gga:374111; -.
DR CTD; 10659; -.
DR VEuPathDB; HostDB:geneid_374111; -.
DR eggNOG; KOG0144; Eukaryota.
DR InParanoid; Q7T2T1; -.
DR OrthoDB; 1209165at2759; -.
DR PhylomeDB; Q7T2T1; -.
DR PRO; PR:Q7T2T1; -.
DR Proteomes; UP000000539; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IDA:AgBase.
DR GO; GO:0005634; C:nucleus; IDA:AgBase.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0042835; F:BRE binding; TAS:AgBase.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0000900; F:mRNA regulatory element binding translation repressor activity; TAS:AgBase.
DR GO; GO:0003676; F:nucleic acid binding; TAS:AgBase.
DR GO; GO:0000166; F:nucleotide binding; TAS:AgBase.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0000380; P:alternative mRNA splicing, via spliceosome; IDA:AgBase.
DR GO; GO:0006376; P:mRNA splice site selection; IBA:GO_Central.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IBA:GO_Central.
DR CDD; cd12631; RRM1_CELF1_2_Bruno; 1.
DR CDD; cd12634; RRM2_CELF1_2; 1.
DR CDD; cd12638; RRM3_CELF1_2; 1.
DR Gene3D; 3.30.70.330; -; 3.
DR InterPro; IPR034196; CELF1/2_RRM1.
DR InterPro; IPR034198; CELF1/2_RRM2.
DR InterPro; IPR034199; CELF1/2_RRM3.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 3.
DR SMART; SM00360; RRM; 3.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50102; RRM; 3.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; mRNA processing; Nucleus;
KW Reference proteome; Repeat; Repressor; RNA-binding.
FT CHAIN 1..484
FT /note="CUGBP Elav-like family member 2"
FT /id="PRO_0000295193"
FT DOMAIN 16..99
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 108..188
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 399..477
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..89
FT /note="Necessary for nuclear export"
FT REGION 90..178
FT /note="Necessary for nuclear export"
FT REGION 188..240
FT /note="Necessary for splicing activity"
FT REGION 347..399
FT /note="Necessary for nuclear localization"
FT REGION 426..484
FT /note="Necessary for nuclear localization and splicing
FT activity"
FT VAR_SEQ 335
FT /note="G -> GTVNS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15226369"
FT /id="VSP_026816"
SQ SEQUENCE 484 AA; 51581 MW; DCBFF7303CF7557E CRC64;
MNGALDHSDQ PDPDAIKTFV GQIPRSWSEK ELKELFEPYG AVYQINVLRD RSQNPPQSKG
CCFVTFYTRK AALEAQNALH NIKTLPGMHH PIQMKPADSE KSNAVEDRKL FIGMVSKKCN
ENDIRVMFSP FGQIEECRIL RGPDGLSRGC AFVTFSTRAM AQNAIKAMHQ SQTMEGCSSP
IVVKFADTQK DKEQRRLQQQ LAQQMQQLNT ATWGNLTGLG GLTPQYLALL QQATSSSNLG
AFSGIQQMAG MNALQLQNLA TLAAAAAAAQ TSATTTNANP LSTTTGALGA LTSPVAASTA
NSTAGAAMNS LTSLGTLQGL AGATVGLNNI NALAGMAALN GGLGATGLTN GTAGTMDALT
QAYSGIQQYA AAALPTLYSQ SLLQQQSAAG SQKEGPEGAN LFIYHLPQEF GDQDILQMFM
PFGNVISAKV FIDKQTNLSK CFGFVSYDNP VSAQAAIQAM NGFQIGMKRL KVQLKRSKND
SKPY
