ID   CELF4_MACFA             Reviewed;         474 AA.
AC   Q4R535;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=CUGBP Elav-like family member 4;
DE            Short=CELF-4;
DE   AltName: Full=Bruno-like protein 4;
DE   AltName: Full=CUG-BP- and ETR-3-like factor 4;
DE   AltName: Full=RNA-binding protein BRUNOL-4;
GN   Name=CELF4; Synonyms=BRUNOL4; ORFNames=QccE-21315;
OS   Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9541;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain cortex;
RG   International consortium for macaque cDNA sequencing and analysis;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications.";
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA-binding protein implicated in the regulation of pre-mRNA
CC       alternative splicing. Mediates exon inclusion and/or exclusion in pre-
CC       mRNA that are subject to tissue-specific and developmentally regulated
CC       alternative splicing. Specifically activates exon 5 inclusion of
CC       cardiac isoforms of TNNT2 during heart remodeling at the juvenile to
CC       adult transition. Promotes exclusion of both the smooth muscle (SM) and
CC       non-muscle (NM) exons in actinin pre-mRNAs. Activates the splicing of
CC       MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE)
CC       intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CELF/BRUNOL family. {ECO:0000305}.
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DR   EMBL; AB169709; BAE01790.1; -; mRNA.
DR   RefSeq; NP_001306316.1; NM_001319387.1.
DR   AlphaFoldDB; Q4R535; -.
DR   SMR; Q4R535; -.
DR   STRING; 9541.XP_005586884.1; -.
DR   PRIDE; Q4R535; -.
DR   GeneID; 101866365; -.
DR   CTD; 56853; -.
DR   eggNOG; KOG0146; Eukaryota.
DR   Proteomes; UP000233100; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0048026; P:positive regulation of mRNA splicing, via spliceosome; ISS:UniProtKB.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   CDD; cd12632; RRM1_CELF3_4_5_6; 1.
DR   Gene3D; 3.30.70.330; -; 3.
DR   InterPro; IPR034648; CELF3/4/5/6_RRM1.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   Pfam; PF00076; RRM_1; 3.
DR   SMART; SM00360; RRM; 3.
DR   SUPFAM; SSF54928; SSF54928; 2.
DR   PROSITE; PS50102; RRM; 3.
PE   2: Evidence at transcript level;
KW   Activator; Cytoplasm; mRNA processing; mRNA splicing; Nucleus;
KW   Reference proteome; Repeat; RNA-binding.
FT   CHAIN           1..474
FT                   /note="CUGBP Elav-like family member 4"
FT                   /id="PRO_0000295222"
FT   DOMAIN          54..135
FT                   /note="RRM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          141..221
FT                   /note="RRM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          392..467
FT                   /note="RRM 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   REGION          1..287
FT                   /note="Sufficient for RNA-binding and MSE-dependent
FT                   splicing activity"
FT                   /evidence="ECO:0000250"
FT   REGION          18..41
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          228..247
FT                   /note="Necessary for TNNT2 exon 5 inclusion"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   474 AA;  50754 MW;  9E87A66CBAEF229C CRC64;
     MYIKMATLAN GQADNASLST NGLGSSPGSA GHMNGLSHSP GNPSTIPMKD HDAIKLFIGQ
     IPRNLDEKDL KPLFEEFGKI YELTVLKDRF TGMHKGCAFL TYCERESALK AQSALHEQKT
     LPGMNRPIQV KPADSESRGD RKLFVGMLNK QQSEDDVRRL FEAFGNIEEC TILRGPDGNS
     KGCAFVKYSS HAEAQAAINA LHGSQTMPGA SSSLVVKFAD TDKERTMRRM QQMAGQMGMF
     NPMAIPFGAY GAYAQALMQQ QAALMASVAQ GGYLNPMAAF AAAQMQQMAA LNMNGLAAAP
     MTPTSGGSTP PGITAPAVPS IPSPIGVNGF TGLPPQANGQ PAAEAVFANG IHPYPAQSPT
     AADPLQQAYA GVQQYAGPAY PAAYGQISQA FPQPPPMIPQ QQREGPEGCN LFIYHLPQEF
     GDAELMQMFL PFGFVSFDNP ASAQTAIQAM NGFQIGMKRL KVQLKRPKDA NRPY