CELF4_PONAB
ID CELF4_PONAB Reviewed; 486 AA.
AC Q5NVC8; Q5R6A4; Q5R8W7;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=CUGBP Elav-like family member 4;
DE Short=CELF-4;
DE AltName: Full=Bruno-like protein 4;
DE AltName: Full=CUG-BP- and ETR-3-like factor 4;
DE AltName: Full=RNA-binding protein BRUNOL-4;
GN Name=CELF4; Synonyms=BRUNOL4;
OS Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Pongo.
OX NCBI_TaxID=9601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC TISSUE=Brain cortex;
RG The German cDNA consortium;
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: RNA-binding protein implicated in the regulation of pre-mRNA
CC alternative splicing. Mediates exon inclusion and/or exclusion in pre-
CC mRNA that are subject to tissue-specific and developmentally regulated
CC alternative splicing. Specifically activates exon 5 inclusion of
CC cardiac isoforms of TNNT2 during heart remodeling at the juvenile to
CC adult transition. Promotes exclusion of both the smooth muscle (SM) and
CC non-muscle (NM) exons in actinin pre-mRNAs. Activates the splicing of
CC MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE)
CC intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q5NVC8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5NVC8-2; Sequence=VSP_026841, VSP_026843;
CC Name=3;
CC IsoId=Q5NVC8-3; Sequence=VSP_026841, VSP_026842, VSP_026843;
CC -!- SIMILARITY: Belongs to the CELF/BRUNOL family. {ECO:0000305}.
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DR EMBL; CR859631; CAH91793.1; -; mRNA.
DR EMBL; CR860590; CAH92712.1; -; mRNA.
DR EMBL; CR926110; CAI29735.1; -; mRNA.
DR RefSeq; NP_001127577.1; NM_001134105.1.
DR RefSeq; NP_001128833.1; NM_001135361.1. [Q5NVC8-3]
DR AlphaFoldDB; Q5NVC8; -.
DR SMR; Q5NVC8; -.
DR STRING; 9601.ENSPPYP00000010242; -.
DR Ensembl; ENSPPYT00000010647; ENSPPYP00000010242; ENSPPYG00000009118. [Q5NVC8-3]
DR Ensembl; ENSPPYT00000055912; ENSPPYP00000035998; ENSPPYG00000009118. [Q5NVC8-2]
DR Ensembl; ENSPPYT00000056896; ENSPPYP00000026335; ENSPPYG00000009118. [Q5NVC8-1]
DR GeneID; 100174655; -.
DR KEGG; pon:100174655; -.
DR CTD; 56853; -.
DR eggNOG; KOG0146; Eukaryota.
DR GeneTree; ENSGT00940000158673; -.
DR InParanoid; Q5NVC8; -.
DR OrthoDB; 1209165at2759; -.
DR Proteomes; UP000001595; Chromosome 18.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0098794; C:postsynapse; IEA:GOC.
DR GO; GO:0003729; F:mRNA binding; IEA:Ensembl.
DR GO; GO:0036002; F:pre-mRNA binding; IEA:Ensembl.
DR GO; GO:0000380; P:alternative mRNA splicing, via spliceosome; IEA:Ensembl.
DR GO; GO:0060079; P:excitatory postsynaptic potential; IEA:Ensembl.
DR GO; GO:0001701; P:in utero embryonic development; IEA:Ensembl.
DR GO; GO:0006376; P:mRNA splice site selection; IEA:Ensembl.
DR GO; GO:0090394; P:negative regulation of excitatory postsynaptic potential; IEA:Ensembl.
DR GO; GO:0048025; P:negative regulation of mRNA splicing, via spliceosome; IEA:Ensembl.
DR GO; GO:0048026; P:positive regulation of mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IEA:Ensembl.
DR GO; GO:1902866; P:regulation of retina development in camera-type eye; IEA:Ensembl.
DR CDD; cd12632; RRM1_CELF3_4_5_6; 1.
DR Gene3D; 3.30.70.330; -; 3.
DR InterPro; IPR034648; CELF3/4/5/6_RRM1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 3.
DR SMART; SM00360; RRM; 3.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50102; RRM; 3.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; Cytoplasm; mRNA processing; mRNA splicing;
KW Nucleus; Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..486
FT /note="CUGBP Elav-like family member 4"
FT /id="PRO_0000295224"
FT DOMAIN 54..135
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 152..232
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 404..479
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..298
FT /note="Sufficient for RNA-binding and MSE-dependent
FT splicing activity"
FT /evidence="ECO:0000250"
FT REGION 18..39
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 121..149
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 239..258
FT /note="Necessary for TNNT2 exon 5 inclusion"
FT /evidence="ECO:0000250"
FT VAR_SEQ 150..151
FT /note="QD -> H (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_026841"
FT VAR_SEQ 268
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_026842"
FT VAR_SEQ 417..444
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_026843"
FT CONFLICT 103
FT /note="C -> S (in Ref. 1; CAH92712)"
FT /evidence="ECO:0000305"
FT CONFLICT 155
FT /note="F -> L (in Ref. 1; CAH92712)"
FT /evidence="ECO:0000305"
FT CONFLICT 210
FT /note="N -> S (in Ref. 1; CAI29735)"
FT /evidence="ECO:0000305"
FT CONFLICT 227
FT /note="V -> A (in Ref. 1; CAI29735)"
FT /evidence="ECO:0000305"
FT CONFLICT 258
FT /note="F -> S (in Ref. 1; CAI29735)"
FT /evidence="ECO:0000305"
FT CONFLICT 354
FT /note="A -> V (in Ref. 1; CAH92712)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 486 AA; 51966 MW; 17EA8A6A6752C565 CRC64;
MYIKMATLAN GQADNASLST NGLGSSPGSA GHMNGLSHSP GNPSTIPMKD HDAIKLFIGQ
IPRNLDEKDL KPLFEEFGKI YELTVLKDRF TGMHKGCAFL TYCERESALK AQSALHEQKT
LPGMNRPIQV KPADSESRGG SSCLRQPPSQ DRKLFVGMLN KQQSEDDVRR LFEAFGNIEE
CTILRGPDGN SKGCAFVKYS SHAEAQAAIN ALHGSQTMPG ASSSLVVKFA DTDKERTMRR
MQQMAGQMGM FNPMAIPFGA YGAYAQALMQ QQAALMASVA QGGYLNPMAA FAAAQMQQMA
ALNMNGLAAA PMTPTSGGST PPGITAPAVP SIPSPIGVNG FTGLPPQANG QPAAEAVFAN
GIHPYPAQSP TAADPLQQAY AGVQQYAGPA AYPAAYGQIS QAFPQPPPMI PQQQREGPEG
CNLFIYHLPQ EFGDAELMQM FLPFGFVSFD NPASAQTAIQ AMNGFQIGMK RLKVQLKRPK
DANRPY
