ID   CEMA_CHLRE              Reviewed;         500 AA.
AC   Q37050; B7U1J2;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Chloroplast envelope membrane protein;
GN   Name=cemA; Synonyms=ycf10;
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CHARACTERIZATION.
RC   STRAIN=cw15;
RX   PubMed=9362486; DOI=10.1093/emboj/16.22.6713;
RA   Rolland N., Dorne A.-J., Amoroso G., Sueltemeyer D.F., Joyard J.,
RA   Rochaix J.-D.;
RT   "Disruption of the plastid ycf10 open reading frame affects uptake of
RT   inorganic carbon in the chloroplast of Chlamydomonas.";
RL   EMBO J. 16:6713-6726(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=137c / CC-125;
RA   Parker J.R., Willey D.L.;
RL   Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CC-503;
RX   PubMed=19473533; DOI=10.1186/1471-2148-9-120;
RA   Smith D.R., Lee R.W.;
RT   "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome:
RT   addressing the mutational-hazard hypothesis.";
RL   BMC Evol. Biol. 9:120-120(2009).
RN   [4]
RP   IDENTIFICATION, AND COMPLETE PLASTID GENOME.
RX   PubMed=12417694; DOI=10.1105/tpc.006155;
RA   Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H.,
RA   Stern D.B.;
RT   "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a
RT   sea of repeats.";
RL   Plant Cell 14:2659-2679(2002).
CC   -!- FUNCTION: May be involved in proton extrusion. Indirectly promotes
CC       efficient inorganic carbon uptake into chloroplasts.
CC       {ECO:0000269|PubMed:9362486}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC       {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Cema family. {ECO:0000305}.
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DR   EMBL; X90559; CAA62148.1; -; Genomic_DNA.
DR   EMBL; X87945; CAA61197.1; -; Genomic_DNA.
DR   EMBL; FJ423446; ACJ50139.1; -; Genomic_DNA.
DR   EMBL; BK000554; DAA00953.1; -; Genomic_DNA.
DR   PIR; S57467; S57467.
DR   RefSeq; NP_958408.1; NC_005353.1.
DR   AlphaFoldDB; Q37050; -.
DR   SMR; Q37050; -.
DR   STRING; 3055.DAA00953; -.
DR   TCDB; 9.B.73.1.1; the chloroplast envelope/cyanobacterial membrane protein (cema) family.
DR   PaxDb; Q37050; -.
DR   PRIDE; Q37050; -.
DR   GeneID; 2717043; -.
DR   KEGG; cre:ChreCp052; -.
DR   eggNOG; ENOG502QV51; Eukaryota.
DR   HOGENOM; CLU_545585_0_0_1; -.
DR   InParanoid; Q37050; -.
DR   OrthoDB; 1276845at2759; -.
DR   Proteomes; UP000006906; Chloroplast.
DR   GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015078; F:proton transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01308; CemA; 1.
DR   InterPro; IPR004282; CemA.
DR   PANTHER; PTHR33650; PTHR33650; 1.
DR   Pfam; PF03040; CemA; 2.
PE   1: Evidence at protein level;
KW   Chloroplast; Hydrogen ion transport; Ion transport; Membrane; Plastid;
KW   Plastid inner membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..500
FT                   /note="Chloroplast envelope membrane protein"
FT                   /id="PRO_0000216637"
FT   TRANSMEM        129..149
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        378..398
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        425..445
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        461..481
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          204..354
FT                   /note="Insert"
SQ   SEQUENCE   500 AA;  57855 MW;  03258E9171C70725 CRC64;
     MYTFIYFKNP KHFNLSQPSR CTAQPFISTR NRPLSCPIPT VQPIYVTHGI ILPILKGTLI
     SQRGVNIVPF VSKRDSSYYT DKDKVVSITY EEIGLFPRSF SRVLDRFLKQ LFSDVDNLVI
     QEYRFYRYLF LTTIKTIFIL FFVPFLVNFA AKNYIVKPIT EYFWNTSHPE IFLNSYEQKR
     AFVELAKFEE KIYFETLVES HSHHQTHRDS KPLRENGIYF PDGEFLDNAN LLSTPRSINS
     NTFLKQNIDI SLREEKPLTL VQGVNLLEEK KELNIPLAQE NIAYNNQSIP QTSFGQGNFS
     SLFTGDREGE ETAKQNLLSQ RVIGANLRQI YLPSAEGEML PSIRGSLDSI KNKDISKIYQ
     EKTIELATYY NNHSIEAITN FFADLLSLFT LLYLLITLEI QINITKSFLL EVFFGLDDSK
     KSLLILLITD LLVGYHSSNL WELFFEFIFN HYGIPESQTG IFLLVATLPV LLDVLFKYLI
     FRHLNRSSPA TVATYQAIIE