ID   CEMA_PEA                Reviewed;         231 AA.
AC   P20150;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Chloroplast envelope membrane protein;
DE   AltName: Full=Heme-binding protein;
GN   Name=cemA {ECO:0000255|HAMAP-Rule:MF_01308}; Synonyms=hbp, ycf10;
OS   Pisum sativum (Garden pea).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2103453; DOI=10.1007/bf00036920;
RA   Willey D.L., Gray J.C.;
RT   "An open reading frame encoding a putative haem-binding polypeptide is
RT   cotranscribed with the pea chloroplast gene for apocytochrome f.";
RL   Plant Mol. Biol. 15:347-356(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Alaska;
RX   PubMed=1807835; DOI=10.1007/bf00317074;
RA   Nagano Y., Matsuno R., Sasaki Y.;
RT   "Sequence and transcriptional analysis of the gene cluster trnQ-zfpA-psaI-
RT   ORF231-petA in pea chloroplasts.";
RL   Curr. Genet. 20:431-436(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-18.
RC   STRAIN=cv. Alaska;
RX   PubMed=1913879; DOI=10.1007/bf00309603;
RA   Smith A.G., Wilson R.J., Kaethner T.M., Willey D.L., Gray J.C.;
RT   "Pea chloroplast genes encoding a 4 kDa polypeptide of photosystem I and a
RT   putative enzyme of C1 metabolism.";
RL   Curr. Genet. 19:403-410(1991).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=8422944; DOI=10.1016/0014-5793(93)81743-j;
RA   Sasaki Y., Sekiguchi K., Nagano Y., Matsuno R.;
RT   "Chloroplast envelope protein encoded by chloroplast genome.";
RL   FEBS Lett. 316:93-98(1993).
CC   -!- FUNCTION: May be involved in proton extrusion. Indirectly promotes
CC       efficient inorganic carbon uptake into chloroplasts.
CC       {ECO:0000255|HAMAP-Rule:MF_01308}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane; Multi-pass
CC       membrane protein.
CC   -!- SIMILARITY: Belongs to the Cema family. {ECO:0000305}.
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DR   EMBL; X53525; CAA37602.1; -; Genomic_DNA.
DR   EMBL; X56315; CAA39758.1; -; Genomic_DNA.
DR   EMBL; X54750; CAA38549.1; -; Genomic_DNA.
DR   PIR; S12124; S12124.
DR   RefSeq; YP_003587556.1; NC_014057.1.
DR   AlphaFoldDB; P20150; -.
DR   SMR; P20150; -.
DR   GeneID; 9073104; -.
DR   GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015078; F:proton transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01308; CemA; 1.
DR   InterPro; IPR004282; CemA.
DR   PANTHER; PTHR33650; PTHR33650; 1.
DR   Pfam; PF03040; CemA; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Hydrogen ion transport; Ion transport; Membrane; Plastid;
KW   Plastid inner membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..231
FT                   /note="Chloroplast envelope membrane protein"
FT                   /id="PRO_0000216655"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01308"
FT   TRANSMEM        104..124
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01308"
FT   TRANSMEM        154..174
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01308"
FT   TRANSMEM        189..209
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01308"
SQ   SEQUENCE   231 AA;  27409 MW;  E6FC971EEC8555B5 CRC64;
     MAKKKAFIPL LCLTSIVFLP WCISFTFKKS LESWITHWYN TKESEIFLNT IQEKSILKKF
     LEFEELFLLD EMLKEYPETR LQNLRIEIYK ETIQLIQTNN QDHIHTILHF CTNIICFLIL
     SVYSIRGNQE LIILNSWVQE FLYNLSDTIK AFSILFLIEF CVGYHSTGGW ELMIGSVYKD
     FGFIPNDHII SFLVSILPAI LDTIFKYWIF RYLNRVSPSL VLIYDSIPFQ E