CENH3_ORYSJ
ID CENH3_ORYSJ Reviewed; 164 AA.
AC Q6T367; B9FKV9; Q6AVM4;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Histone H3-like centromeric protein CENH3 {ECO:0000303|PubMed:14716315};
DE AltName: Full=Centromeric histone CENH3 {ECO:0000305};
DE AltName: Full=Centromeric histone H3 {ECO:0000305};
GN Name=CENH3 {ECO:0000303|PubMed:14716315};
GN OrderedLocusNames=Os05g0489800 {ECO:0000312|EMBL:BAS94646.1},
GN LOC_Os05g41080 {ECO:0000305};
GN ORFNames=OJ1119_H02.19 {ECO:0000312|EMBL:AAT77291.1},
GN OsJ_19013 {ECO:0000312|EMBL:EEE64181.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947 {ECO:0000312|EMBL:AAR85315.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC TISSUE=Panicle;
RX PubMed=14716315; DOI=10.1038/ng1289;
RA Nagaki K., Cheng Z., Ouyang S., Talbert P.B., Kim M., Jones K.M.,
RA Henikoff S., Buell C.R., Jiang J.;
RT "Sequencing of a rice centromere uncovers active genes.";
RL Nat. Genet. 36:138-145(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16877494; DOI=10.1105/tpc.106.043794;
RA Yan H., Ito H., Nobuta K., Ouyang S., Jin W., Tian S., Lu C., Venu R.C.,
RA Wang G.L., Green P.J., Wing R.A., Buell C.R., Meyers B.C., Jiang J.;
RT "Genomic and genetic characterization of rice Cen3 reveals extensive
RT transcription and evolutionary implications of a complex centromere.";
RL Plant Cell 18:2123-2133(2006).
CC -!- FUNCTION: Histone H3-like variant which exclusively replaces
CC conventional H3 in the nucleosome core of centromeric chromatin at the
CC inner plate of the kinetochore (PubMed:14716315, PubMed:16877494).
CC Required for recruitment and assembly of kinetochore proteins, mitotic
CC progression and chromosome segregation (Probable).
CC {ECO:0000269|PubMed:14716315, ECO:0000269|PubMed:16877494,
CC ECO:0000305|PubMed:14716315, ECO:0000305|PubMed:16877494}.
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere, kinetochore
CC {ECO:0000269|PubMed:14716315, ECO:0000269|PubMed:16877494}.
CC Note=Localizes in the kinetochore domain of centromeres.
CC {ECO:0000269|PubMed:14716315, ECO:0000269|PubMed:16877494}.
CC -!- SIMILARITY: Belongs to the histone H3 family. {ECO:0000305}.
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DR EMBL; AY438639; AAR85315.1; -; mRNA.
DR EMBL; AC097175; AAT77291.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF17808.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS94646.1; -; Genomic_DNA.
DR EMBL; CM000142; EEE64181.1; -; Genomic_DNA.
DR RefSeq; XP_015638738.1; XM_015783252.1.
DR AlphaFoldDB; Q6T367; -.
DR SMR; Q6T367; -.
DR STRING; 4530.OS05T0489800-01; -.
DR PaxDb; Q6T367; -.
DR PRIDE; Q6T367; -.
DR EnsemblPlants; Os05t0489800-01; Os05t0489800-01; Os05g0489800.
DR GeneID; 4339159; -.
DR Gramene; Os05t0489800-01; Os05t0489800-01; Os05g0489800.
DR eggNOG; KOG1745; Eukaryota.
DR HOGENOM; CLU_078295_3_0_1; -.
DR InParanoid; Q6T367; -.
DR OMA; ISHFYAP; -.
DR OrthoDB; 1564596at2759; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000007752; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0030527; F:structural constituent of chromatin; IEA:InterPro.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR009072; Histone-fold.
DR InterPro; IPR007125; Histone_H2A/H2B/H3.
DR InterPro; IPR000164; Histone_H3/CENP-A.
DR PANTHER; PTHR11426; PTHR11426; 1.
DR Pfam; PF00125; Histone; 1.
DR PRINTS; PR00622; HISTONEH3.
DR SMART; SM00428; H3; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
DR PROSITE; PS00959; HISTONE_H3_2; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Centromere; Chromosome; DNA-binding; Kinetochore; Methylation;
KW Nucleosome core; Reference proteome.
FT CHAIN 1..164
FT /note="Histone H3-like centromeric protein CENH3"
FT /id="PRO_0000455447"
FT REGION 1..71
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..59
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 5
FT /note="N6,N6,N6-trimethyllysine; alternate"
FT /evidence="ECO:0000250|UniProtKB:P59226"
FT MOD_RES 5
FT /note="N6,N6-dimethyllysine; alternate"
FT /evidence="ECO:0000250|UniProtKB:P59226"
FT MOD_RES 5
FT /note="N6-methyllysine; alternate"
FT /evidence="ECO:0000250|UniProtKB:P59226"
FT MOD_RES 19
FT /note="N6-acetyllysine; alternate"
FT /evidence="ECO:0000250|UniProtKB:P59226"
FT MOD_RES 19
FT /note="N6-methyllysine; alternate"
FT /evidence="ECO:0000250|UniProtKB:P59226"
FT MOD_RES 30
FT /note="N6,N6,N6-trimethyllysine; alternate"
FT /evidence="ECO:0000250|UniProtKB:P59226"
FT MOD_RES 30
FT /note="N6,N6-dimethyllysine; alternate"
FT /evidence="ECO:0000250|UniProtKB:P59226"
FT MOD_RES 30
FT /note="N6-methyllysine; alternate"
FT /evidence="ECO:0000250|UniProtKB:P59226"
SQ SEQUENCE 164 AA; 18582 MW; D0D5EE233D0E3415 CRC64;
MARTKHPAVR KSKAEPKKKL QFERSPRPSK AQRAGGGTGT SATTRSAAGT SASGTPRQQT
KQRKPHRFRP GTVALREIRK FQKTTELLIP FAPFSRLVRE ITDFYSKDVS RWTLEALLAL
QEAAEYHLVD IFEVSNLCAI HAKRVTIMQK DMQLARRIGG RRPW
