ACCO_PEA
ID ACCO_PEA Reviewed; 317 AA.
AC P31239;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=1-aminocyclopropane-1-carboxylate oxidase;
DE Short=ACC oxidase;
DE EC=1.14.17.4;
DE AltName: Full=Ethylene-forming enzyme;
DE Short=EFE;
GN Name=ACO;
OS Pisum sativum (Garden pea).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX NCBI_TaxID=3888;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Alaska; TISSUE=Shoot;
RX PubMed=8278511; DOI=10.1104/pp.101.2.689;
RA Peck S.C., Olson D.C., Kende H.;
RT "A cDNA sequence encoding 1-aminocyclopropane-1-carboxylate oxidase from
RT pea.";
RL Plant Physiol. 101:689-690(1993).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 = CO2 +
CC ethene + 2 H2O + hydrogen cyanide + L-dehydroascorbate;
CC Xref=Rhea:RHEA:23640, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:18153, ChEBI:CHEBI:18407,
CC ChEBI:CHEBI:38290, ChEBI:CHEBI:58360, ChEBI:CHEBI:58539;
CC EC=1.14.17.4;
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC -!- PATHWAY: Alkene biosynthesis; ethylene biosynthesis via S-adenosyl-L-
CC methionine; ethylene from S-adenosyl-L-methionine: step 2/2.
CC -!- DEVELOPMENTAL STAGE: Expressed during fruit ripening.
CC -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC family. {ECO:0000305}.
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DR EMBL; M98357; AAA33644.1; -; mRNA.
DR PIR; T06544; T06544.
DR AlphaFoldDB; P31239; -.
DR SMR; P31239; -.
DR PRIDE; P31239; -.
DR EnsemblPlants; Psat1g177520.1; Psat1g177520.1.cds; Psat1g177520.
DR Gramene; Psat1g177520.1; Psat1g177520.1.cds; Psat1g177520.
DR UniPathway; UPA00384; UER00563.
DR GO; GO:0009815; F:1-aminocyclopropane-1-carboxylate oxidase activity; IEA:UniProtKB-EC.
DR GO; GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009693; P:ethylene biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0009835; P:fruit ripening; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.330; -; 1.
DR InterPro; IPR026992; DIOX_N.
DR InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR InterPro; IPR027443; IPNS-like_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR Pfam; PF14226; DIOX_N; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 2: Evidence at transcript level;
KW Ethylene biosynthesis; Fruit ripening; Iron; Metal-binding; Oxidoreductase;
KW Vitamin C.
FT CHAIN 1..317
FT /note="1-aminocyclopropane-1-carboxylate oxidase"
FT /id="PRO_0000067269"
FT DOMAIN 153..253
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 177
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 179
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 234
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
SQ SEQUENCE 317 AA; 36054 MW; 7F71D09ECF2C2A6F CRC64;
MENFPIVDMG KLNTEDRKST MELIKDACEN WGFFECVNHG ISIEMMDTVE KLTKEHYKKC
MEQRFKEMVA TKGLECVQSE IDDLDWESTF FLRHLPVSSI SEIPDLDDDY RKVMKEFALK
LEELAEELLD LLCENLGLEK GYLKKAFYGS KGPNFGTKVS NYPPCPKPEL IKGLRAHTDA
GGIILLFQDD KVSGLQLLKD DQWIDVPPMR HSIVINLGDQ LEVITNGKYK SVMHRVIAQT
DGARMSIASF YNPGDDAVIS PASTLLKENE TSEVYPKFVF DDYMKLYMGL KFQAKEPRFE
AMMKAMSSVK VGPVVSI
