CENPA_ASHGO
ID CENPA_ASHGO Reviewed; 204 AA.
AC Q75DE4;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2013, sequence version 2.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Histone H3-like centromeric protein CSE4;
DE AltName: Full=CENP-A homolog;
GN Name=CSE4; OrderedLocusNames=ABR083C;
OS Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS (Yeast) (Eremothecium gossypii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX NCBI_TaxID=284811;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=15001715; DOI=10.1126/science.1095781;
RA Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA Gaffney T.D., Philippsen P.;
RT "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT cerevisiae genome.";
RL Science 304:304-307(2004).
RN [2]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 88.
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=23749448; DOI=10.1534/g3.112.002881;
RA Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT loci, numerous translocations, lack of transposons, and distinct gene
RT duplications.";
RL G3 (Bethesda) 3:1225-1239(2013).
CC -!- FUNCTION: Histone H3-like variant which exclusively replaces
CC conventional H3 in the nucleosome core of centromeric chromatin at the
CC inner plate of the kinetochore. Required for recruitment and assembly
CC of kinetochore proteins, mitotic progression and chromosome
CC segregation. May serve as an epigenetic mark that propagates centromere
CC identity through replication and cell division (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of the core kinetochore. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere
CC {ECO:0000250}. Chromosome, centromere, kinetochore {ECO:0000250}.
CC Note=Localizes exclusively in the kinetochore domain of centromeres.
CC {ECO:0000250}.
CC -!- PTM: Ubiquitinated. Is degraded through ubiquitin mediated proteolysis
CC when not protected by its association to the kinetochore. This may
CC ensure exclusive localization of CSE4 to the kinetochore (By
CC similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the histone H3 family. {ECO:0000305}.
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DR EMBL; AE016815; AAS50853.2; -; Genomic_DNA.
DR RefSeq; NP_983029.2; NM_208382.2.
DR AlphaFoldDB; Q75DE4; -.
DR SMR; Q75DE4; -.
DR STRING; 33169.AAS50853; -.
DR EnsemblFungi; AAS50853; AAS50853; AGOS_ABR083C.
DR GeneID; 4619133; -.
DR KEGG; ago:AGOS_ABR083C; -.
DR eggNOG; KOG1745; Eukaryota.
DR HOGENOM; CLU_078295_3_0_1; -.
DR InParanoid; Q75DE4; -.
DR OMA; KRITIMR; -.
DR Proteomes; UP000000591; Chromosome II.
DR GO; GO:0005729; C:2-micrometer circle DNA; IEA:EnsemblFungi.
DR GO; GO:0043505; C:CENP-A containing nucleosome; IEA:EnsemblFungi.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0019237; F:centromeric DNA binding; IEA:EnsemblFungi.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0030527; F:structural constituent of chromatin; IEA:InterPro.
DR GO; GO:0030543; P:2-micrometer plasmid partitioning; IEA:EnsemblFungi.
DR GO; GO:0051382; P:kinetochore assembly; IEA:EnsemblFungi.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IEA:EnsemblFungi.
DR GO; GO:0061644; P:protein localization to CENP-A containing chromatin; IEA:EnsemblFungi.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR009072; Histone-fold.
DR InterPro; IPR007125; Histone_H2A/H2B/H3.
DR InterPro; IPR000164; Histone_H3/CENP-A.
DR PANTHER; PTHR11426; PTHR11426; 1.
DR Pfam; PF00125; Histone; 1.
DR PRINTS; PR00622; HISTONEH3.
DR SMART; SM00428; H3; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
DR PROSITE; PS00959; HISTONE_H3_2; 1.
PE 3: Inferred from homology;
KW Centromere; Chromosome; DNA-binding; Kinetochore; Nucleus;
KW Reference proteome; Ubl conjugation.
FT CHAIN 1..204
FT /note="Histone H3-like centromeric protein CSE4"
FT /id="PRO_0000270595"
FT REGION 68..107
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 89..202
FT /note="H3-like"
FT COMPBIAS 68..95
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 204 AA; 23919 MW; B14952BA3A278CF3 CRC64;
MEQSMSSEQA ERVAGRVEQL GGFQNNESIN QRALLLLQRN RQRRQLLQRQ EDRTRYIPDD
AKRRVVERVA EPAAAESRAD GAREEERAAR PARPRETRRI AKKPQRYRPS DVALQEIRRY
QRSTELLISR MPFARLVKEV TDQFTTVDQQ MRWQSMAILA LQEASEAYIV GLLEHTNLLA
LHAKRVTVMR KDMQLARRIR GQFI