ID   CENPA_DANRE             Reviewed;         145 AA.
AC   Q803H4;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Histone H3-like centromeric protein A;
DE   AltName: Full=Centromere protein A;
DE            Short=CENP-A;
GN   Name=cenpa;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=AB;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY.
RC   TISSUE=Embryo;
RX   PubMed=18307296; DOI=10.1021/pr700667w;
RA   Lemeer S., Pinkse M.W.H., Mohammed S., van Breukelen B., den Hertog J.,
RA   Slijper M., Heck A.J.R.;
RT   "Online automated in vivo zebrafish phosphoproteomics: from large-scale
RT   analysis down to a single embryo.";
RL   J. Proteome Res. 7:1555-1564(2008).
CC   -!- FUNCTION: Histone H3-like nucleosomal protein that is specifically
CC       found in centromeric nucleosomes. Replaces conventional H3 in the
CC       nucleosome core of centromeric chromatin at the inner plate of the
CC       kinetochore. The presence of CENPA subtly modifies the nucleosome
CC       structure and the way DNA is wrapped around the nucleosome and gives
CC       rise to protruding DNA ends that are less well-ordered and rigid
CC       compared to nucleosomes containing histone H3. May serve as an
CC       epigenetic mark that propagates centromere identity through replication
CC       and cell division. Required for recruitment and assembly of kinetochore
CC       proteins, and as a consequence required for progress through mitosis,
CC       chromosome segregation and cytokinesis. {ECO:0000250|UniProtKB:P49450}.
CC   -!- SUBUNIT: Component of centromeric nucleosomes, where DNA is wrapped
CC       around a histone octamer core. The octamer contains two molecules each
CC       of H2A, H2B, CENPA and H4 assembled in one CENPA-H4 heterotetramer and
CC       two H2A-H2B heterodimers. CENPA modulates the DNA-binding
CC       characteristics of nucleosomes so that protruding DNA ends have higher
CC       flexibility than in nucleosomes containing conventional histone H3.
CC       {ECO:0000250|UniProtKB:P49450}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P49450}.
CC       Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:P49450}.
CC       Chromosome, centromere {ECO:0000250|UniProtKB:P49450}. Note=Localizes
CC       exclusively in the kinetochore domain of centromeres. Occupies a
CC       compact domain at the inner kinetochore plate stretching across 2
CC       thirds of the length of the constriction but encompassing only one
CC       third of the constriction width and height.
CC       {ECO:0000250|UniProtKB:P49450}.
CC   -!- SIMILARITY: Belongs to the histone H3 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC044483; AAH44483.1; -; mRNA.
DR   RefSeq; NP_001157712.1; NM_001164240.1.
DR   AlphaFoldDB; Q803H4; -.
DR   SMR; Q803H4; -.
DR   STRING; 7955.ENSDARP00000126317; -.
DR   iPTMnet; Q803H4; -.
DR   PaxDb; Q803H4; -.
DR   PRIDE; Q803H4; -.
DR   Ensembl; ENSDART00000181048; ENSDARP00000153106; ENSDARG00000093022.
DR   GeneID; 100302659; -.
DR   KEGG; dre:100302659; -.
DR   CTD; 1058; -.
DR   eggNOG; KOG1745; Eukaryota.
DR   GeneTree; ENSGT01050000244889; -.
DR   InParanoid; Q803H4; -.
DR   OrthoDB; 1564596at2759; -.
DR   TreeFam; TF354293; -.
DR   PRO; PR:Q803H4; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 8.
DR   Bgee; ENSDARG00000093022; Expressed in somite and 49 other tissues.
DR   ExpressionAtlas; Q803H4; baseline and differential.
DR   GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR   GO; GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR   GO; GO:0030527; F:structural constituent of chromatin; IEA:InterPro.
DR   Gene3D; 1.10.20.10; -; 1.
DR   InterPro; IPR009072; Histone-fold.
DR   InterPro; IPR007125; Histone_H2A/H2B/H3.
DR   InterPro; IPR000164; Histone_H3/CENP-A.
DR   Pfam; PF00125; Histone; 1.
DR   SMART; SM00428; H3; 1.
DR   SUPFAM; SSF47113; SSF47113; 1.
DR   PROSITE; PS00959; HISTONE_H3_2; 1.
PE   1: Evidence at protein level;
KW   Centromere; Chromosome; DNA-binding; Kinetochore; Nucleosome core; Nucleus;
KW   Phosphoprotein; Reference proteome.
FT   CHAIN           1..145
FT                   /note="Histone H3-like centromeric protein A"
FT                   /id="PRO_0000249469"
FT   REGION          1..54
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          51..145
FT                   /note="H3-like"
FT   COMPBIAS        1..16
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         19
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18307296"
SQ   SEQUENCE   145 AA;  16681 MW;  053967EBEB14B146 CRC64;
     MPRHTSAHKR KPSTPRRRSP PASLPPPAGS RTRRHSGPSG SSPRKKHKFR PGTRALMEIR
     KYQKSTGLLL RKAPFSRLVR EVCQMFSREH MMWQGYALMA LQEAAEAFMV RLFSDANLCA
     IHAKRVTLFP RDIQLARRIR GVEHM