CENPA_KLULA
ID CENPA_KLULA Reviewed; 184 AA.
AC Q6CTI2;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Histone H3-like centromeric protein CSE4;
DE AltName: Full=CENP-A homolog;
DE AltName: Full=Chromosome segregation protein 4;
GN Name=CSE4; OrderedLocusNames=KLLA0C12529g;
OS Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX NCBI_TaxID=284590;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
CC -!- FUNCTION: Histone H3-like variant which exclusively replaces
CC conventional H3 in the nucleosome core of centromeric chromatin at the
CC inner plate of the kinetochore. Required for recruitment and assembly
CC of kinetochore proteins, mitotic progression and chromosome
CC segregation. May serve as an epigenetic mark that propagates centromere
CC identity through replication and cell division (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of the core kinetochore. {ECO:0000250}.
CC -!- INTERACTION:
CC Q6CTI2; Q6CMU6: KLLA0_E17601g; NbExp=4; IntAct=EBI-15928212, EBI-15928236;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere
CC {ECO:0000250}. Chromosome, centromere, kinetochore {ECO:0000250}.
CC Note=Localizes exclusively in the kinetochore domain of centromeres.
CC {ECO:0000250}.
CC -!- PTM: Ubiquitinated. Is degraded through ubiquitin mediated proteolysis
CC when not protected by its association to the kinetochore. This may
CC ensure exclusive localization of CSE4 to the kinetochore (By
CC similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the histone H3 family. {ECO:0000305}.
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DR EMBL; CR382123; CAH01608.1; -; Genomic_DNA.
DR RefSeq; XP_452757.1; XM_452757.1.
DR PDB; 2YFV; X-ray; 2.32 A; A=81-180.
DR PDB; 2YFW; X-ray; 2.60 A; A/C/E/G=93-184.
DR PDBsum; 2YFV; -.
DR PDBsum; 2YFW; -.
DR AlphaFoldDB; Q6CTI2; -.
DR SMR; Q6CTI2; -.
DR DIP; DIP-60345N; -.
DR IntAct; Q6CTI2; 2.
DR STRING; 28985.XP_452757.1; -.
DR EnsemblFungi; CAH01608; CAH01608; KLLA0_C12529g.
DR GeneID; 2891972; -.
DR KEGG; kla:KLLA0_C12529g; -.
DR eggNOG; KOG1745; Eukaryota.
DR HOGENOM; CLU_078295_3_0_1; -.
DR InParanoid; Q6CTI2; -.
DR OMA; KRITIMR; -.
DR Proteomes; UP000000598; Chromosome C.
DR GO; GO:0005729; C:2-micrometer circle DNA; IEA:EnsemblFungi.
DR GO; GO:0043505; C:CENP-A containing nucleosome; IEA:EnsemblFungi.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0019237; F:centromeric DNA binding; IEA:EnsemblFungi.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0030527; F:structural constituent of chromatin; IEA:InterPro.
DR GO; GO:0030543; P:2-micrometer plasmid partitioning; IEA:EnsemblFungi.
DR GO; GO:0051382; P:kinetochore assembly; IEA:EnsemblFungi.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IEA:EnsemblFungi.
DR GO; GO:0061644; P:protein localization to CENP-A containing chromatin; IEA:EnsemblFungi.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR009072; Histone-fold.
DR InterPro; IPR007125; Histone_H2A/H2B/H3.
DR InterPro; IPR000164; Histone_H3/CENP-A.
DR PANTHER; PTHR11426; PTHR11426; 1.
DR Pfam; PF00125; Histone; 1.
DR PRINTS; PR00622; HISTONEH3.
DR SMART; SM00428; H3; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
DR PROSITE; PS00959; HISTONE_H3_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Centromere; Chromosome; DNA-binding; Kinetochore; Nucleus;
KW Reference proteome; Ubl conjugation.
FT CHAIN 1..184
FT /note="Histone H3-like centromeric protein CSE4"
FT /id="PRO_0000270598"
FT REGION 54..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 82..182
FT /note="H3-like"
FT HELIX 94..101
FT /evidence="ECO:0007829|PDB:2YFW"
FT HELIX 110..122
FT /evidence="ECO:0007829|PDB:2YFV"
FT HELIX 135..162
FT /evidence="ECO:0007829|PDB:2YFV"
FT STRAND 166..168
FT /evidence="ECO:0007829|PDB:2YFV"
FT HELIX 170..178
FT /evidence="ECO:0007829|PDB:2YFV"
SQ SEQUENCE 184 AA; 21410 MW; D9507DA4E536DCD9 CRC64;
MEQSIRSIDG SRSLSNVGAS LIDRESINQR ALQLLQRNRR RRLLLNRSED KARYIQPERS
ASSQQIHPPE HHISAHERIT KARGTRYKPT DLALAEIRKY QRSTDLLISR MPFARLVKEV
TDQFTTESEP LRWQSMAIMA LQEASEAYLV GLLEHTNLLA LHAKRITIMR KDMQLARRIR
GQFI
