ACCO_PRUMU
ID ACCO_PRUMU Reviewed; 319 AA.
AC Q9MB94;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=1-aminocyclopropane-1-carboxylate oxidase;
DE Short=ACC oxidase;
DE EC=1.14.17.4;
DE AltName: Full=Ethylene-forming enzyme;
DE Short=EFE;
GN Name=ACO1;
OS Prunus mume (Japanese apricot) (Armeniaca mume).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Amygdaleae; Prunus.
OX NCBI_TaxID=102107;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Mita S., Kirita C., Kato M., Hyodo H.;
RT "Expression of ACC synthase is enhanced earlier than that of ACC oxidase
RT during fruit ripening of mume (Prunus mume).";
RL Physiol. Plantarum 107:319-328(1999).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 = CO2 +
CC ethene + 2 H2O + hydrogen cyanide + L-dehydroascorbate;
CC Xref=Rhea:RHEA:23640, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:18153, ChEBI:CHEBI:18407,
CC ChEBI:CHEBI:38290, ChEBI:CHEBI:58360, ChEBI:CHEBI:58539;
CC EC=1.14.17.4;
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC -!- PATHWAY: Alkene biosynthesis; ethylene biosynthesis via S-adenosyl-L-
CC methionine; ethylene from S-adenosyl-L-methionine: step 2/2.
CC -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC family. {ECO:0000305}.
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DR EMBL; AB031027; BAA90550.1; -; mRNA.
DR RefSeq; NP_001280183.1; NM_001293254.1.
DR AlphaFoldDB; Q9MB94; -.
DR SMR; Q9MB94; -.
DR PRIDE; Q9MB94; -.
DR GeneID; 103329345; -.
DR KEGG; pmum:103329345; -.
DR UniPathway; UPA00384; UER00563.
DR GO; GO:0009815; F:1-aminocyclopropane-1-carboxylate oxidase activity; IEA:UniProtKB-EC.
DR GO; GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009693; P:ethylene biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0009835; P:fruit ripening; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.330; -; 1.
DR InterPro; IPR026992; DIOX_N.
DR InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR InterPro; IPR027443; IPNS-like_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR Pfam; PF14226; DIOX_N; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 2: Evidence at transcript level;
KW Ethylene biosynthesis; Fruit ripening; Iron; Metal-binding; Oxidoreductase;
KW Vitamin C.
FT CHAIN 1..319
FT /note="1-aminocyclopropane-1-carboxylate oxidase"
FT /id="PRO_0000067274"
FT DOMAIN 153..253
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 177
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 179
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 234
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
SQ SEQUENCE 319 AA; 36243 MW; 31697F96C89F7DB2 CRC64;
MENFPIINLE GLNGEGRKAT MEKIKDACEN WGFFELVSHG IPTEFLDTVE RLTKEHYRQC
LEQRFKELVA SKGLEAVKTE VNDMDWESTF YLRHLPKSNI SEVPDLEDQY RNVMKEFALK
LEKLAEQLLD LLCENLGLEK GYLKKAFYGT NGPTFGTKVS NYPPCPNPEL IKGLRAHTDA
GGLILLFQDD KVSGLQLLKD GQWIDVPPMR HSIVINLGDQ LEVITNGKYR SVEHRVIAQT
DGTRMSIASF YNPGSDAVIY PAPTLVEKEA EEKNQVYPKF VFEDYMKLYA GLKFQPKEPR
FEAMKAVETN ISLVPIATA