ID   CENPA_SCHPO             Reviewed;         120 AA.
AC   Q9Y812;
DT   13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 148.
DE   RecName: Full=Histone H3-like centromeric protein cnp1;
DE            Short=Centromere protein 1;
DE   AltName: Full=CENP-A homolog;
DE   AltName: Full=Silencing in the middle of the centromere protein 2;
GN   Name=cnp1; Synonyms=sim2; ORFNames=SPBC1105.17;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH MIS6 AND
RP   SIM4, AND SUBCELLULAR LOCATION.
RX   PubMed=10864871; DOI=10.1126/science.288.5474.2215;
RA   Takahashi K., Chen E.S., Yanagida M.;
RT   "Requirement of Mis6 centromere connector for localizing a CENP-A-like
RT   protein in fission yeast.";
RL   Science 288:2215-2219(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: Histone H3-like variant which exclusively replaces
CC       conventional H3 in the nucleosome core of centromeric chromatin at the
CC       inner plate of the kinetochore. Required for recruitment and assembly
CC       of kinetochore proteins, mitotic progression and chromosome
CC       segregation. May serve as an epigenetic mark that propagates centromere
CC       identity through replication and cell division.
CC       {ECO:0000269|PubMed:10864871}.
CC   -!- SUBUNIT: Component of the core kinetochore. Interacts with mis6 and
CC       sim4. {ECO:0000269|PubMed:10864871}.
CC   -!- INTERACTION:
CC       Q9Y812; Q9Y738: mis12; NbExp=3; IntAct=EBI-1153281, EBI-1002822;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10864871}.
CC       Chromosome, centromere, kinetochore {ECO:0000269|PubMed:10864871}.
CC       Note=Localizes exclusively in the kinetochore domain of centromeres.
CC   -!- PTM: Ubiquitinated. Is degraded through ubiquitin mediated proteolysis
CC       when not protected by its association to the kinetochore. This may
CC       ensure exclusive localization of cnp1 to the kinetochore.
CC   -!- SIMILARITY: Belongs to the histone H3 family. {ECO:0000305}.
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DR   EMBL; AB041724; BAA94760.1; -; Genomic_DNA.
DR   EMBL; CU329671; CAB50980.1; -; Genomic_DNA.
DR   PIR; T39294; T39294.
DR   RefSeq; NP_596473.1; NM_001022392.2.
DR   AlphaFoldDB; Q9Y812; -.
DR   SMR; Q9Y812; -.
DR   BioGRID; 276213; 43.
DR   IntAct; Q9Y812; 6.
DR   STRING; 4896.SPBC1105.17.1; -.
DR   PaxDb; Q9Y812; -.
DR   PRIDE; Q9Y812; -.
DR   EnsemblFungi; SPBC1105.17.1; SPBC1105.17.1:pep; SPBC1105.17.
DR   GeneID; 2539658; -.
DR   KEGG; spo:SPBC1105.17; -.
DR   PomBase; SPBC1105.17; cnp1.
DR   VEuPathDB; FungiDB:SPBC1105.17; -.
DR   eggNOG; KOG1745; Eukaryota.
DR   HOGENOM; CLU_078295_7_1_1; -.
DR   InParanoid; Q9Y812; -.
DR   OMA; RTTDIQP; -.
DR   PhylomeDB; Q9Y812; -.
DR   PRO; PR:Q9Y812; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0061638; C:CENP-A containing chromatin; IDA:PomBase.
DR   GO; GO:0043505; C:CENP-A containing nucleosome; NAS:PomBase.
DR   GO; GO:0000785; C:chromatin; IDA:PomBase.
DR   GO; GO:0034506; C:chromosome, centromeric core domain; IDA:PomBase.
DR   GO; GO:0000775; C:chromosome, centromeric region; IDA:PomBase.
DR   GO; GO:0000779; C:condensed chromosome, centromeric region; IDA:PomBase.
DR   GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0019237; F:centromeric DNA binding; ISM:PomBase.
DR   GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR   GO; GO:0030527; F:structural constituent of chromatin; IEA:InterPro.
DR   GO; GO:0061641; P:CENP-A containing chromatin organization; IMP:PomBase.
DR   Gene3D; 1.10.20.10; -; 1.
DR   InterPro; IPR009072; Histone-fold.
DR   InterPro; IPR007125; Histone_H2A/H2B/H3.
DR   InterPro; IPR000164; Histone_H3/CENP-A.
DR   PANTHER; PTHR11426; PTHR11426; 1.
DR   Pfam; PF00125; Histone; 1.
DR   PRINTS; PR00622; HISTONEH3.
DR   SMART; SM00428; H3; 1.
DR   SUPFAM; SSF47113; SSF47113; 1.
PE   1: Evidence at protein level;
KW   Centromere; Chromosome; DNA-binding; Kinetochore; Nucleus;
KW   Reference proteome; Ubl conjugation.
FT   CHAIN           1..120
FT                   /note="Histone H3-like centromeric protein cnp1"
FT                   /id="PRO_0000221375"
FT   REGION          1..26
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          14..120
FT                   /note="H3-like"
SQ   SEQUENCE   120 AA;  13884 MW;  D013696368AE4601 CRC64;
     MAKKSLMAEP GDPIPRPRKK RYRPGTTALR EIRKYQRSTD LLIQRLPFSR IVREISSEFV
     ANFSTDVGLR WQSTALQCLQ EAAEAFLVHL FEDTNLCAIH AKRVTIMQRD MQLARRIRGA