ID   CENPA_TETTS             Reviewed;         187 AA.
AC   Q2N2K6;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Histone H3-like centromeric protein CNA1;
GN   Name=CNA1; ORFNames=TTHERM_00146340;
OS   Tetrahymena thermophila (strain SB210).
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC   Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae;
OC   Tetrahymena.
OX   NCBI_TaxID=312017;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=B2086, CU427, and CU428;
RX   PubMed=16251352; DOI=10.1091/mbc.e05-07-0698;
RA   Cervantes M.D., Xi X., Vermaak D., Yao M.C., Malik H.S.;
RT   "The CNA1 histone of the ciliate Tetrahymena thermophila is essential for
RT   chromosome segregation in the germline micronucleus.";
RL   Mol. Biol. Cell 17:485-497(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SB210;
RX   PubMed=16933976; DOI=10.1371/journal.pbio.0040286;
RA   Eisen J.A., Coyne R.S., Wu M., Wu D., Thiagarajan M., Wortman J.R.,
RA   Badger J.H., Ren Q., Amedeo P., Jones K.M., Tallon L.J., Delcher A.L.,
RA   Salzberg S.L., Silva J.C., Haas B.J., Majoros W.H., Farzad M.,
RA   Carlton J.M., Smith R.K. Jr., Garg J., Pearlman R.E., Karrer K.M., Sun L.,
RA   Manning G., Elde N.C., Turkewitz A.P., Asai D.J., Wilkes D.E., Wang Y.,
RA   Cai H., Collins K., Stewart B.A., Lee S.R., Wilamowska K., Weinberg Z.,
RA   Ruzzo W.L., Wloga D., Gaertig J., Frankel J., Tsao C.-C., Gorovsky M.A.,
RA   Keeling P.J., Waller R.F., Patron N.J., Cherry J.M., Stover N.A.,
RA   Krieger C.J., del Toro C., Ryder H.F., Williamson S.C., Barbeau R.A.,
RA   Hamilton E.P., Orias E.;
RT   "Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a
RT   model eukaryote.";
RL   PLoS Biol. 4:1620-1642(2006).
RN   [3]
RP   FUNCTION.
RC   STRAIN=B2086, and CU428;
RX   PubMed=16738316; DOI=10.1128/mcb.00079-06;
RA   Cui B., Gorovsky M.A.;
RT   "Centromeric histone H3 is essential for vegetative cell division and for
RT   DNA elimination during conjugation in Tetrahymena thermophila.";
RL   Mol. Cell. Biol. 26:4499-4510(2006).
CC   -!- FUNCTION: Histone H3-like variant which exclusively replaces
CC       conventional H3 in the nucleosome core of centromeric chromatin at the
CC       inner plate of the kinetochore. Required for recruitment and assembly
CC       of kinetochore proteins, mitotic progression and chromosome
CC       segregation. May serve as an epigenetic mark that propagates centromere
CC       identity through replication and cell division.
CC       {ECO:0000269|PubMed:16738316}.
CC   -!- SUBUNIT: Component of the core kinetochore. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere, kinetochore.
CC       Note=Localizes exclusively in the kinetochore domain of centromeres.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the histone H3 family. {ECO:0000305}.
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DR   EMBL; DQ126145; AAZ91458.1; -; Genomic_DNA.
DR   EMBL; GG662793; EAR91028.1; -; Genomic_DNA.
DR   RefSeq; XP_001011273.1; XM_001011273.1.
DR   AlphaFoldDB; Q2N2K6; -.
DR   SMR; Q2N2K6; -.
DR   STRING; 5911.EAR91028; -.
DR   EnsemblProtists; EAR91028; EAR91028; TTHERM_00146340.
DR   GeneID; 7840155; -.
DR   KEGG; tet:TTHERM_00146340; -.
DR   eggNOG; KOG1745; Eukaryota.
DR   HOGENOM; CLU_078295_3_0_1; -.
DR   InParanoid; Q2N2K6; -.
DR   OMA; VEDYMVS; -.
DR   OrthoDB; 1564596at2759; -.
DR   Proteomes; UP000009168; Unassembled WGS sequence.
DR   GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR   GO; GO:0000786; C:nucleosome; IEA:InterPro.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR   GO; GO:0030527; F:structural constituent of chromatin; IEA:InterPro.
DR   Gene3D; 1.10.20.10; -; 1.
DR   InterPro; IPR009072; Histone-fold.
DR   InterPro; IPR007125; Histone_H2A/H2B/H3.
DR   InterPro; IPR000164; Histone_H3/CENP-A.
DR   PANTHER; PTHR11426; PTHR11426; 1.
DR   Pfam; PF00125; Histone; 1.
DR   PRINTS; PR00622; HISTONEH3.
DR   SMART; SM00428; H3; 1.
DR   SUPFAM; SSF47113; SSF47113; 1.
PE   3: Inferred from homology;
KW   Centromere; Chromosome; DNA-binding; Kinetochore; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..187
FT                   /note="Histone H3-like centromeric protein CNA1"
FT                   /id="PRO_0000385013"
FT   REGION          1..76
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        8..22
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        45..76
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   187 AA;  21703 MW;  E2D93D4F26402D39 CRC64;
     MARKAYQPKR RSNSNQNQQR SDSLKKNKQD NLRSKSAGNQ QGNEKNKKDI QDQRNKASTK
     KKRESSGEKY ESARDKVIRR FRPGDNALKQ LRQYNQTPSL LIRKLPFQRL IREISTRMTE
     EDSLRWTSFA LVLLQTVVED YMVSFFEDAN ACALHAKRVT LMSKDLALAA RIRGQKNVTG
     IFIPTKK