ID   CENPI_RAT               Reviewed;         745 AA.
AC   Q63517;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Centromere protein I;
DE            Short=CENP-I;
DE   AltName: Full=FSH primary response protein 1;
DE   AltName: Full=Follicle-stimulating hormone primary response protein;
DE   AltName: Full=Leucine-rich primary response protein 1;
GN   Name=Cenpi; Synonyms=Fshprh1, Lrpr1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Wistar; TISSUE=Testis;
RX   PubMed=7758824; DOI=10.1016/0303-7207(94)03468-9;
RA   Slegtenhorst-Eegdeman K.E., Post M., Baarends W.M., Themmen A.P.M.,
RA   Grootegoed J.A.;
RT   "Regulation of gene expression in Sertoli cells by follicle-stimulating
RT   hormone (FSH): cloning and characterization of LRPR1, a primary response
RT   gene encoding a leucine-rich protein.";
RL   Mol. Cell. Endocrinol. 108:115-124(1995).
CC   -!- FUNCTION: Component of the CENPA-CAD (nucleosome distal) complex, a
CC       complex recruited to centromeres which is involved in assembly of
CC       kinetochore proteins, mitotic progression and chromosome segregation.
CC       May be involved in incorporation of newly synthesized CENPA into
CC       centromeres via its interaction with the CENPA-NAC complex. Required
CC       for the localization of CENPF, MAD1L1 and MAD2 (MAD2L1 or MAD2L2) to
CC       kinetochores. Involved in the response of gonadal tissues to follicle-
CC       stimulating hormone (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the CENPA-CAD complex, composed of CENPI, CENPK,
CC       CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex
CC       interacts with the CENPA-NAC complex, at least composed of CENPA,
CC       CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. Interacts with SENP6 (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere
CC       {ECO:0000250}. Note=Localizes exclusively in the centromeres. The
CC       CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC
CC       complex (By similarity). {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in testis, ovary and spleen. A
CC       much lower mRNA level is found in brain and lung, and no expression is
CC       detected in liver, kidney, heart, muscle, pituitary gland, prostate,
CC       epididymis and seminal vesicle.
CC   -!- INDUCTION: By follicle-stimulating hormone (FSH).
CC   -!- PTM: Sumoylated. Sumoylated form can be polyubiquitinated by RNF4,
CC       leading to its degradation. Desumoylation by SENP6 prevents its
CC       degradation (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CENP-I/CTF3 family. {ECO:0000305}.
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DR   EMBL; X90355; CAA62018.1; -; mRNA.
DR   PIR; I57665; I57665.
DR   RefSeq; NP_037087.1; NM_012955.1.
DR   AlphaFoldDB; Q63517; -.
DR   STRING; 10116.ENSRNOP00000041317; -.
DR   PhosphoSitePlus; Q63517; -.
DR   PaxDb; Q63517; -.
DR   GeneID; 25448; -.
DR   KEGG; rno:25448; -.
DR   UCSC; RGD:2631; rat.
DR   CTD; 2491; -.
DR   RGD; 2631; Cenpi.
DR   eggNOG; ENOG502QU9H; Eukaryota.
DR   InParanoid; Q63517; -.
DR   PhylomeDB; Q63517; -.
DR   Reactome; R-RNO-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR   Reactome; R-RNO-2467813; Separation of Sister Chromatids.
DR   Reactome; R-RNO-2500257; Resolution of Sister Chromatid Cohesion.
DR   Reactome; R-RNO-5663220; RHO GTPases Activate Formins.
DR   Reactome; R-RNO-606279; Deposition of new CENPA-containing nucleosomes at the centromere.
DR   Reactome; R-RNO-68877; Mitotic Prometaphase.
DR   Reactome; R-RNO-9648025; EML4 and NUDC in mitotic spindle formation.
DR   PRO; PR:Q63517; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0000776; C:kinetochore; ISO:RGD.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0034508; P:centromere complex assembly; IEA:InterPro.
DR   GO; GO:0042699; P:follicle-stimulating hormone signaling pathway; IEP:RGD.
DR   InterPro; IPR012485; CENP-I.
DR   Pfam; PF07778; CENP-I; 1.
PE   2: Evidence at transcript level;
KW   Centromere; Chromosome; Nucleus; Reference proteome; Ubl conjugation.
FT   CHAIN           1..745
FT                   /note="Centromere protein I"
FT                   /id="PRO_0000087356"
FT   REGION          1..58
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..27
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   745 AA;  85651 MW;  3C9D1AD066D4FD85 CRC64;
     MATPRLTRNS QQQNRISQGS NSRQTTLLDW KVKDKAGNSK SVLEESSSLE DSNHADDQTE
     DALQTAVEYF QKGPKKASLS KDSVLEKHLK TVENVAWNNG LAPEAIDILL NVALSGKFGN
     ALSTRILKCM IPETHISEDS VVKAVSWLCV GKCSGNTKVL FYRWLVAMFD FIDHKKQINS
     LYGFFFVSLQ DDTLCPYVCH LLYLLTKKEN VKPFRARKLL DLQAKMGMQP HLQALLSLYK
     FFAPTLISVS LPVRKKIFFN NSKNLWTPAL LAVKLRNQGI FPEPLKLQLG PTSGRSLKRK
     WNYHSVIPAV NSAKKECREK MSLFDYLSND RSLPVEQLQS FPQLLENIHC LELPSQMSSV
     LNSSLLLHYV NCVKDESILL RLSYWLSQTL QEECVWYNMN DYQQEKEFIN FLDMVIRVQC
     FLQEGFYSCE AFLYKSLPLW DGFSCRSQFL KLLAWIPFSS FSEIKPLLLD HLAPLFFTSS
     IYFKCSLLQC LKDLLQNWLL WLSTEAQVQP MTDSPLETTL GGSMDSVSQL IEYIGWLSMV
     AIRLESSSTL LVHFILDFYE KVCDIYINYD LPLVVLFPPV VFHSAFLSLD ATILNQLCYI
     MYRYRDNLTA AKKKDLVQKA KSEFSISSKI CKEFNYYLTA LVHCLWSSRP FETGVYIDPQ
     IIENTGETQY KHNLNFVHHP CLLNYAASFL LQESPEEMPV HLSSIRGKKW NWYLDHLYSE
     GFQGLNLFIK SSIDHSSVSK TEKNT