ID   CENPI_SCHPO             Reviewed;         672 AA.
AC   P87227;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 151.
DE   RecName: Full=Inner kinetochore subunit mis6;
DE   AltName: Full=CENP-I homolog;
DE   AltName: Full=Constitutive centromere-associated network protein mis6;
DE   AltName: Full=Sim4 complex subunit mis6;
GN   Name=mis6; ORFNames=SPAC1687.20c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=9230309; DOI=10.1016/s0092-8674(00)80320-7;
RA   Saitoh S., Takahashi K., Yanagida M.;
RT   "Mis6, a fission yeast inner centromere protein, acts during G1/S and forms
RT   specialized chromatin required for equal segregation.";
RL   Cell 90:131-143(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [3]
RP   CHARACTERIZATION.
RX   PubMed=10398680; DOI=10.1101/gad.13.13.1664;
RA   Goshima G., Saitoh S., Yanagida M.;
RT   "Proper metaphase spindle length is determined by centromere proteins Mis12
RT   and Mis6 required for faithful chromosome segregation.";
RL   Genes Dev. 13:1664-1677(1999).
RN   [4]
RP   FUNCTION, AND INTERACTION WITH CNP1.
RX   PubMed=10864871; DOI=10.1126/science.288.5474.2215;
RA   Takahashi K., Chen E.S., Yanagida M.;
RT   "Requirement of Mis6 centromere connector for localizing a CENP-A-like
RT   protein in fission yeast.";
RL   Science 288:2215-2219(2000).
RN   [5]
RP   INTERACTION WITH SIM4.
RX   PubMed=12719471; DOI=10.1083/jcb.200212110;
RA   Pidoux A.L., Richardson W., Allshire R.C.;
RT   "Sim4: a novel fission yeast kinetochore protein required for centromeric
RT   silencing and chromosome segregation.";
RL   J. Cell Biol. 161:295-307(2003).
RN   [6]
RP   INTERACTION WITH MIS15 AND MIS17.
RX   PubMed=15369671; DOI=10.1016/j.cell.2004.09.002;
RA   Hayashi T., Fujita Y., Iwasaki O., Adachi Y., Takahashi K., Yanagida M.;
RT   "Mis16 and Mis18 are required for CENP-A loading and histone deacetylation
RT   at centromeres.";
RL   Cell 118:715-729(2004).
RN   [7]
RP   FUNCTION, AND IDENTIFICATION IN THE SIM4 COMPLEX.
RX   PubMed=16079914; DOI=10.1038/sj.emboj.7600762;
RA   Liu X., McLeod I., Anderson S., Yates J.R. III, He X.;
RT   "Molecular analysis of kinetochore architecture in fission yeast.";
RL   EMBO J. 24:2919-2930(2005).
RN   [8]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
CC   -!- FUNCTION: Component of the kinetochore, a multiprotein complex that
CC       assembles on centromeric DNA and attaches chromosomes to spindle
CC       microtubules, mediating chromosome segregation and sister chromatid
CC       segregation during meiosis and mitosis. Component of the inner
CC       kinetochore constitutive centromere-associated network (CCAN), which
CC       serves as a structural platform for outer kinetochore assembly
CC       (PubMed:10398680). Required for the localization of cnp1 to the
CC       centromere (PubMed:10864871). {ECO:0000269|PubMed:10398680,
CC       ECO:0000269|PubMed:10864871}.
CC   -!- SUBUNIT: Component of the inner kinetochore constitutive centromere-
CC       associated network (CCAN) (also known as central kinetochore Sim4
CC       complex in fission yeast), which is composed of at least cnl2, cnp3,
CC       cnp20, fta1, fta2, fta3, fta4, fta6, fta7, mal2, mhf1, mhf2, mis6,
CC       mis15, mis17, sim4 and wip1 (PubMed:16079914). Interacts with cnp1,
CC       sim4, mis15 and mis17 (PubMed:10864871, PubMed:12719471,
CC       PubMed:15369671). {ECO:0000269|PubMed:10864871,
CC       ECO:0000269|PubMed:12719471, ECO:0000269|PubMed:15369671,
CC       ECO:0000269|PubMed:16079914}.
CC   -!- INTERACTION:
CC       P87227; O94494: sim4; NbExp=5; IntAct=EBI-1148555, EBI-1148540;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16823372}.
CC       Chromosome, centromere {ECO:0000269|PubMed:16823372}.
CC   -!- SIMILARITY: Belongs to the CENP-I/CTF3 family. {ECO:0000305}.
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DR   EMBL; AB004690; BAA20458.1; -; Genomic_DNA.
DR   EMBL; CU329670; CAA22614.1; -; Genomic_DNA.
DR   PIR; T37763; T37763.
DR   RefSeq; NP_593139.1; NM_001018535.2.
DR   AlphaFoldDB; P87227; -.
DR   SMR; P87227; -.
DR   BioGRID; 278771; 47.
DR   IntAct; P87227; 4.
DR   STRING; 4896.SPAC1687.20c.1; -.
DR   MaxQB; P87227; -.
DR   PaxDb; P87227; -.
DR   EnsemblFungi; SPAC1687.20c.1; SPAC1687.20c.1:pep; SPAC1687.20c.
DR   GeneID; 2542304; -.
DR   KEGG; spo:SPAC1687.20c; -.
DR   PomBase; SPAC1687.20c; mis6.
DR   VEuPathDB; FungiDB:SPAC1687.20c; -.
DR   eggNOG; ENOG502QU9H; Eukaryota.
DR   HOGENOM; CLU_408895_0_0_1; -.
DR   InParanoid; P87227; -.
DR   OMA; MTENQSH; -.
DR   PhylomeDB; P87227; -.
DR   PRO; PR:P87227; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0034506; C:chromosome, centromeric core domain; IDA:PomBase.
DR   GO; GO:0000775; C:chromosome, centromeric region; IDA:PomBase.
DR   GO; GO:0000779; C:condensed chromosome, centromeric region; IDA:PomBase.
DR   GO; GO:0000939; C:inner kinetochore; IDA:PomBase.
DR   GO; GO:0000776; C:kinetochore; IDA:PomBase.
DR   GO; GO:0031511; C:Mis6-Sim4 complex; IDA:PomBase.
DR   GO; GO:0005634; C:nucleus; IC:PomBase.
DR   GO; GO:0140483; F:kinetochore adaptor activity; IPI:PomBase.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0034080; P:CENP-A containing chromatin assembly; IMP:PomBase.
DR   GO; GO:0051382; P:kinetochore assembly; TAS:PomBase.
DR   GO; GO:0000070; P:mitotic sister chromatid segregation; IMP:PomBase.
DR   InterPro; IPR012485; CENP-I.
DR   Pfam; PF07778; CENP-I; 2.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Mitosis; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..672
FT                   /note="Inner kinetochore subunit mis6"
FT                   /id="PRO_0000096493"
SQ   SEQUENCE   672 AA;  78513 MW;  4E2B7FEDD567174F CRC64;
     MESFENKGFL DIEEGIQWIK KNSENLSSSK KTILARLQQF HKLCSEVGLN QHSISSLLDV
     ILSKKTHFDK NHVQLLIKCL YPNELISQTI AIRIISSLDP HGLRCSYAIQ AKLLNWLIHV
     YEFLDGNNLL CRYYGVLFHF LDFLTLRPYI SNLLVLLTKH YHVKSFRIHQ LLALYQKPGN
     TADPYLLALI LTYKQHFPDV IVGSYTYRKH GSVRLDSEWI AATKAILNRQ SEDVPLETWS
     SEKRKRQSSL IPDLITMKNT SSSYSLEELT SVQQMGLVYE KIVFPSRIAA VLKSKLFLIF
     LFLKNKNVYY SRLDEWLHIT LNYGLALRSG SNNQEEEVLH LLYKYLLFSP KFPKSLLQYV
     ITFFSKPNIT EENYNLLTLL VTHIPITTDS SYFNSLLKEF EQFILQKNAE FCSKHLNILW
     LWLFRMLNLR IASMGNNHTL LEKCLLITNH ATFLVSHFSW DVSLAYQLSR LFQLYYKILT
     KIRKQIEPNI PPKELIYVLF FQPSAFYINS MVGLLLLTKN YQERLMDSRI DAISKFTHSY
     LKSLSEIILL KEKRAILSFL QLWEPFKSDY SQFLPIATRI ANDHPYAQRV FSLTCAPQFF
     SYINGYQIYL QQTNPATGSI PLKPIQEETF GAFQSNLHLS DSWEDFQKNF IIYLKKKGYL
     AISDFLLSTL NR