ID   CENPK_SCHPO             Reviewed;         277 AA.
AC   O94494;
DT   13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Inner kinetochore subunit sim4;
DE   AltName: Full=CENP-K homolog;
DE   AltName: Full=Constitutive centromere-associated network protein sim4;
DE   AltName: Full=Silencing in the middle of the centromere protein 4;
GN   Name=sim4; ORFNames=SPBC18E5.03c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   FUNCTION, INTERACTION WITH MIS6, AND SUBCELLULAR LOCATION.
RX   PubMed=12719471; DOI=10.1083/jcb.200212110;
RA   Pidoux A.L., Richardson W., Allshire R.C.;
RT   "Sim4: a novel fission yeast kinetochore protein required for centromeric
RT   silencing and chromosome segregation.";
RL   J. Cell Biol. 161:295-307(2003).
RN   [3]
RP   FUNCTION, IDENTIFICATION IN THE SIM4 COMPLEX, AND INTERACTION WITH DAD1.
RX   PubMed=16079914; DOI=10.1038/sj.emboj.7600762;
RA   Liu X., McLeod I., Anderson S., Yates J.R. III, He X.;
RT   "Molecular analysis of kinetochore architecture in fission yeast.";
RL   EMBO J. 24:2919-2930(2005).
RN   [4]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
CC   -!- FUNCTION: Component of the kinetochore, a multiprotein complex that
CC       assembles on centromeric DNA and attaches chromosomes to spindle
CC       microtubules, mediating chromosome segregation and sister chromatid
CC       segregation during meiosis and mitosis. Component of the inner
CC       kinetochore constitutive centromere-associated network (CCAN), which
CC       serves as a structural platform for outer kinetochore assembly.
CC       {ECO:0000269|PubMed:12719471, ECO:0000269|PubMed:16079914}.
CC   -!- SUBUNIT: Component of the inner kinetochore constitutive centromere-
CC       associated network (CCAN) (also known as central kinetochore Sim4
CC       complex in fission yeast), which is composed of at least cnl2, cnp3,
CC       cnp20, fta1, fta2, fta3, fta4, fta6, fta7, mal2, mhf1, mhf2, mis6,
CC       mis15, mis17, sim4 and wip1 (PubMed:16079914). Interacts with mis6 and
CC       dad1 (PubMed:12719471, PubMed:16079914). {ECO:0000269|PubMed:12719471,
CC       ECO:0000269|PubMed:16079914}.
CC   -!- INTERACTION:
CC       O94494; P87227: mis6; NbExp=5; IntAct=EBI-1148540, EBI-1148555;
CC   -!- SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere. Note=Associates
CC       with the central core region of the centromere.
CC   -!- SIMILARITY: Belongs to the CENP-K/MCM22 family. {ECO:0000305}.
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DR   EMBL; CU329671; CAA22663.1; -; Genomic_DNA.
DR   PIR; T39754; T39754.
DR   RefSeq; NP_595849.1; NM_001021753.2.
DR   AlphaFoldDB; O94494; -.
DR   SMR; O94494; -.
DR   BioGRID; 277323; 23.
DR   IntAct; O94494; 1.
DR   STRING; 4896.SPBC18E5.03c.1; -.
DR   MaxQB; O94494; -.
DR   PaxDb; O94494; -.
DR   EnsemblFungi; SPBC18E5.03c.1; SPBC18E5.03c.1:pep; SPBC18E5.03c.
DR   GeneID; 2540804; -.
DR   KEGG; spo:SPBC18E5.03c; -.
DR   PomBase; SPBC18E5.03c; sim4.
DR   VEuPathDB; FungiDB:SPBC18E5.03c; -.
DR   HOGENOM; CLU_1001702_0_0_1; -.
DR   InParanoid; O94494; -.
DR   OMA; FANINQS; -.
DR   PhylomeDB; O94494; -.
DR   PRO; PR:O94494; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0000779; C:condensed chromosome, centromeric region; IDA:PomBase.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0000939; C:inner kinetochore; IDA:PomBase.
DR   GO; GO:0031511; C:Mis6-Sim4 complex; IDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0051382; P:kinetochore assembly; TAS:PomBase.
DR   GO; GO:0000070; P:mitotic sister chromatid segregation; IMP:PomBase.
DR   InterPro; IPR020993; Centromere_CenpK.
DR   PANTHER; PTHR14401; PTHR14401; 1.
DR   Pfam; PF11802; CENP-K; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Mitosis;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..277
FT                   /note="Inner kinetochore subunit sim4"
FT                   /id="PRO_0000097761"
FT   COILED          96..138
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   277 AA;  31730 MW;  6BFD01C3415C6520 CRC64;
     MNSENASLFR NILNDTENLV QPLTQDESRL ALFESLVQSD QLLVEKIEKW EKKVSKFTEN
     SKSEKRKLQL ELFQEIIDLK KCIAFKPIIG KNAIDNNISD LKKNLHSNKK LEAVLKEELH
     QIKKFSSDLQ SLKSSMGERQ KQQAAMSRRG KKSIIHSAII QEKERYEKES QELFTFLDGF
     LDEHAPSLLE GAQASIDKNR PGKNQPSLNF ENISKKDLTE EFKQVIENLM NNSIIPGSPY
     IEVKNERIVS FLVLASLCTV DPQDPSKVKL IPFSDEI