CENPL_YEAST
ID CENPL_YEAST Reviewed; 245 AA.
AC P38265; D6VQA6;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Inner kinetochore subunit IML3 {ECO:0000305};
DE AltName: Full=CENP-L homolog {ECO:0000303|PubMed:22561346};
DE AltName: Full=Constitutive centromere-associated network protein IML3 {ECO:0000305};
DE AltName: Full=Increased minichromosome loss protein 3;
DE AltName: Full=Minichromosome maintenance protein 19;
GN Name=IML3; Synonyms=MCM19; OrderedLocusNames=YBR107C; ORFNames=YBR0836;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7900426; DOI=10.1002/yea.320101014;
RA Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.;
RT "Analysis of a 70 kb region on the right arm of yeast chromosome II.";
RL Yeast 10:1363-1381(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION.
RX PubMed=11361335; DOI=10.1007/s004380000408;
RA Ghosh S.K., Poddar A., Hajra S., Sanyal K., Sinha P.;
RT "The IML3/MCM19 gene of Saccharomyces cerevisiae is required for a
RT kinetochore-related process during chromosome segregation.";
RL Mol. Genet. Genomics 265:249-257(2001).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND COMPONENT OF CTF19 COMPLEX.
RX PubMed=12408861; DOI=10.1016/s0092-8674(02)00973-x;
RA Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S.,
RA Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G.;
RT "Phospho-regulation of kinetochore-microtubule attachments by the Aurora
RT kinase Ipl1p.";
RL Cell 111:163-172(2002).
RN [6]
RP INTERACTION WITH CHL4 AND CTF19, AND SUBCELLULAR LOCATION.
RX PubMed=12589047; DOI=10.1091/mbc.e02-08-0517;
RA Pot I., Measday V., Snydsman B., Cagney G., Fields S., Davis T.N.,
RA Muller E.G.D., Hieter P.;
RT "Chl4p and Iml3p are two new members of the budding yeast outer
RT kinetochore.";
RL Mol. Biol. Cell 14:460-476(2003).
RN [7]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [9]
RP FUNCTION.
RX PubMed=15241623; DOI=10.1007/s00294-004-0516-6;
RA Ghosh S.K., Sau S., Lahiri S., Lohia A., Sinha P.;
RT "The Iml3 protein of the budding yeast is required for the prevention of
RT precocious sister chromatid separation in meiosis I and for sister
RT chromatid disjunction in meiosis II.";
RL Curr. Genet. 46:82-91(2004).
RN [10]
RP IDENTIFICATION IN CCAN, AND SUBUNIT.
RX PubMed=22561346; DOI=10.1038/ncb2493;
RA Schleiffer A., Maier M., Litos G., Lampert F., Hornung P., Mechtler K.,
RA Westermann S.;
RT "CENP-T proteins are conserved centromere receptors of the Ndc80 complex.";
RL Nat. Cell Biol. 14:604-613(2012).
RN [11]
RP X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS).
RX PubMed=24311582; DOI=10.1107/s0907444913022397;
RA Guo Q., Tao Y., Liu H., Teng M., Li X.;
RT "Structural insights into the role of the Chl4-Iml3 complex in kinetochore
RT assembly.";
RL Acta Crystallogr. D 69:2412-2419(2013).
RN [12]
RP X-RAY CRYSTALLOGRAPHY (2.28 ANGSTROMS) OF 1-242 IN COMPLEX WITH CHL4.
RX PubMed=24075991; DOI=10.1016/j.celrep.2013.08.036;
RA Hinshaw S.M., Harrison S.C.;
RT "An Iml3-Chl4 heterodimer links the core centromere to factors required for
RT accurate chromosome segregation.";
RL Cell Rep. 5:29-36(2013).
CC -!- FUNCTION: Component of the kinetochore, a multiprotein complex that
CC assembles on centromeric DNA and attaches chromosomes to spindle
CC microtubules, mediating chromosome segregation and sister chromatid
CC segregation during meiosis and mitosis. Component of the inner
CC kinetochore constitutive centromere-associated network (CCAN), which
CC serves as a structural platform for outer kinetochore assembly.
CC {ECO:0000269|PubMed:11361335, ECO:0000269|PubMed:15241623}.
CC -!- SUBUNIT: Forms a heterodimer with IML3 (PubMed:12589047,
CC PubMed:24075991). CHL4-IML3 is part of a larger constitutive
CC centromere-associated network (CCAN) (also known as central kinetochore
CC CTF19 complex in yeast), which is composed of at least AME1, CHL4,
CC CNN1, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, MHF1, MHF2, MIF2, NKP1,
CC NKP2, OKP1 and WIP1 (PubMed:12408861, PubMed:22561346). Interacts with
CC CHL4 and CTF19 (PubMed:12589047). {ECO:0000269|PubMed:12408861,
CC ECO:0000269|PubMed:12589047, ECO:0000269|PubMed:22561346,
CC ECO:0000269|PubMed:24075991}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere, kinetochore.
CC Note=Associated with kinetochores.
CC -!- MISCELLANEOUS: Present with 125 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the CENP-L/IML3 family. {ECO:0000305}.
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DR EMBL; X78993; CAA55610.1; -; Genomic_DNA.
DR EMBL; Z35976; CAA85062.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07226.1; -; Genomic_DNA.
DR PIR; S48272; S48272.
DR RefSeq; NP_009665.3; NM_001178455.3.
DR PDB; 4IT3; X-ray; 2.50 A; A=1-245.
DR PDB; 4JE3; X-ray; 2.28 A; A=1-242.
DR PDB; 4KR1; X-ray; 2.50 A; A=1-245.
DR PDB; 6NUW; EM; 4.25 A; B=1-245.
DR PDB; 6QLD; EM; 4.15 A; L=2-242.
DR PDB; 6QLE; EM; 3.55 A; L=1-245.
DR PDB; 6QLF; EM; 3.45 A; L=1-245.
DR PDBsum; 4IT3; -.
DR PDBsum; 4JE3; -.
DR PDBsum; 4KR1; -.
DR PDBsum; 6NUW; -.
DR PDBsum; 6QLD; -.
DR PDBsum; 6QLE; -.
DR PDBsum; 6QLF; -.
DR AlphaFoldDB; P38265; -.
DR SMR; P38265; -.
DR BioGRID; 32811; 191.
DR ComplexPortal; CPX-1156; Central kinetochore CTF19 complex.
DR DIP; DIP-5360N; -.
DR IntAct; P38265; 30.
DR MINT; P38265; -.
DR STRING; 4932.YBR107C; -.
DR PaxDb; P38265; -.
DR PRIDE; P38265; -.
DR EnsemblFungi; YBR107C_mRNA; YBR107C; YBR107C.
DR GeneID; 852404; -.
DR KEGG; sce:YBR107C; -.
DR SGD; S000000311; IML3.
DR VEuPathDB; FungiDB:YBR107C; -.
DR eggNOG; ENOG502S57G; Eukaryota.
DR HOGENOM; CLU_1143196_0_0_1; -.
DR InParanoid; P38265; -.
DR OMA; HTKIMRE; -.
DR BioCyc; YEAST:G3O-29069-MON; -.
DR PRO; PR:P38265; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38265; protein.
DR GO; GO:0000776; C:kinetochore; IPI:ComplexPortal.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000940; C:outer kinetochore; IDA:SGD.
DR GO; GO:0030437; P:ascospore formation; IMP:SGD.
DR GO; GO:0008608; P:attachment of spindle microtubules to kinetochore; IC:ComplexPortal.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0034089; P:establishment of meiotic sister chromatid cohesion; IMP:SGD.
DR GO; GO:0034087; P:establishment of mitotic sister chromatid cohesion; IMP:SGD.
DR GO; GO:0034090; P:maintenance of meiotic sister chromatid cohesion; IMP:SGD.
DR GO; GO:0045144; P:meiotic sister chromatid segregation; IMP:SGD.
DR GO; GO:0071459; P:protein localization to chromosome, centromeric region; IMP:SGD.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cell division; Centromere; Chromosome;
KW Kinetochore; Meiosis; Mitosis; Nucleus; Reference proteome.
FT CHAIN 1..245
FT /note="Inner kinetochore subunit IML3"
FT /id="PRO_0000084182"
FT STRAND 3..12
FT /evidence="ECO:0007829|PDB:4JE3"
FT HELIX 16..18
FT /evidence="ECO:0007829|PDB:4IT3"
FT HELIX 21..26
FT /evidence="ECO:0007829|PDB:4JE3"
FT STRAND 31..41
FT /evidence="ECO:0007829|PDB:4JE3"
FT STRAND 46..60
FT /evidence="ECO:0007829|PDB:4JE3"
FT HELIX 67..69
FT /evidence="ECO:0007829|PDB:4JE3"
FT STRAND 72..77
FT /evidence="ECO:0007829|PDB:4JE3"
FT STRAND 81..83
FT /evidence="ECO:0007829|PDB:4JE3"
FT STRAND 85..91
FT /evidence="ECO:0007829|PDB:4JE3"
FT HELIX 95..107
FT /evidence="ECO:0007829|PDB:4JE3"
FT STRAND 111..113
FT /evidence="ECO:0007829|PDB:4JE3"
FT HELIX 118..128
FT /evidence="ECO:0007829|PDB:4JE3"
FT STRAND 130..132
FT /evidence="ECO:0007829|PDB:4JE3"
FT STRAND 134..136
FT /evidence="ECO:0007829|PDB:6QLF"
FT STRAND 138..140
FT /evidence="ECO:0007829|PDB:4JE3"
FT STRAND 145..149
FT /evidence="ECO:0007829|PDB:4JE3"
FT HELIX 154..156
FT /evidence="ECO:0007829|PDB:4JE3"
FT STRAND 160..165
FT /evidence="ECO:0007829|PDB:4JE3"
FT HELIX 167..175
FT /evidence="ECO:0007829|PDB:4JE3"
FT HELIX 183..186
FT /evidence="ECO:0007829|PDB:4JE3"
FT HELIX 188..196
FT /evidence="ECO:0007829|PDB:4JE3"
FT TURN 200..202
FT /evidence="ECO:0007829|PDB:4JE3"
FT STRAND 205..210
FT /evidence="ECO:0007829|PDB:4JE3"
FT TURN 211..213
FT /evidence="ECO:0007829|PDB:4JE3"
FT STRAND 214..219
FT /evidence="ECO:0007829|PDB:4JE3"
FT STRAND 221..227
FT /evidence="ECO:0007829|PDB:4JE3"
FT HELIX 230..239
FT /evidence="ECO:0007829|PDB:4JE3"
SQ SEQUENCE 245 AA; 28066 MW; 813F26C923155D8F CRC64;
MPYTWKFLGI SKQLSLENGI AKLNQLLNLE VDLDIQTIRV PSDPDGGTAA DEYIRYEMRL
DISNLDEGTY SKFIFLGNSK MEVPMFLCYC GTDNRNEVVL QWLKAEYGVI MWPIKFEQKT
MIKLADASIV HVTKENIEQI TWFSSKLYFE PETQDKNLRQ FSIEIPRESC EGLALGYGNT
MHPYNDAIVP YIYNETGMAV ERLPLTSVIL AGHTKIMRES IVTSTRSLRN RVLAVVLQSI
QFTSE