CENPM_MOUSE
ID CENPM_MOUSE Reviewed; 180 AA.
AC Q9CQA0; Q3THN3; Q91VS6;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Centromere protein M;
DE Short=CENP-M;
DE AltName: Full=Proliferation-associated nuclear element protein 1;
GN Name=Cenpm; Synonyms=Pane1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND SUBCELLULAR LOCATION.
RC STRAIN=C57BL/6J; TISSUE=Lactating mammary gland;
RX PubMed=12881717; DOI=10.1038/sj.onc.1206596;
RA Renou J.-P., Bierie B., Miyoshi K., Cui Y., Djiane J., Reichenstein M.,
RA Shani M., Hennighausen L.;
RT "Identification of genes differentially expressed in mouse mammary
RT epithelium transformed by an activated beta-catenin.";
RL Oncogene 22:4594-4610(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and DBA/2J; TISSUE=Embryonic liver;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the CENPA-NAC (nucleosome-associated) complex, a
CC complex that plays a central role in assembly of kinetochore proteins,
CC mitotic progression and chromosome segregation. The CENPA-NAC complex
CC recruits the CENPA-CAD (nucleosome distal) complex and may be involved
CC in incorporation of newly synthesized CENPA into centromeres (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the CENPA-NAC complex, at least composed of
CC CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC
CC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK,
CC CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12881717}. Cytoplasm
CC {ECO:0000269|PubMed:12881717}. Chromosome, centromere, kinetochore
CC {ECO:0000250}. Note=Nuclear in non-confluent cells and cytoplasmic in
CC confluent or dividing cells. Localizes in the kinetochore domain of
CC centromeres (By similarity). {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9CQA0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9CQA0-2; Sequence=VSP_020440;
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DR EMBL; AY243536; AAO62567.1; -; mRNA.
DR EMBL; AY243537; AAO62568.1; -; mRNA.
DR EMBL; AK010885; BAB27246.1; -; mRNA.
DR EMBL; AK011191; BAB27456.1; -; mRNA.
DR EMBL; AK011227; BAB27479.1; -; mRNA.
DR EMBL; AK011464; BAB27635.1; -; mRNA.
DR EMBL; AK011755; BAB27823.1; -; mRNA.
DR EMBL; AK168203; BAE40163.1; -; mRNA.
DR EMBL; AK017679; BAB30869.1; -; mRNA.
DR EMBL; BC009160; AAH09160.1; -; mRNA.
DR CCDS; CCDS27685.1; -. [Q9CQA0-1]
DR CCDS; CCDS37156.1; -. [Q9CQA0-2]
DR RefSeq; NP_001073627.1; NM_001080158.1.
DR RefSeq; NP_079915.1; NM_025639.4. [Q9CQA0-1]
DR RefSeq; NP_840000.2; NM_178269.3. [Q9CQA0-2]
DR AlphaFoldDB; Q9CQA0; -.
DR SMR; Q9CQA0; -.
DR ComplexPortal; CPX-5704; Kinetochore CCAN complex.
DR IntAct; Q9CQA0; 1.
DR MINT; Q9CQA0; -.
DR STRING; 10090.ENSMUSP00000086560; -.
DR PhosphoSitePlus; Q9CQA0; -.
DR EPD; Q9CQA0; -.
DR MaxQB; Q9CQA0; -.
DR PaxDb; Q9CQA0; -.
DR PRIDE; Q9CQA0; -.
DR ProteomicsDB; 280063; -. [Q9CQA0-1]
DR ProteomicsDB; 280064; -. [Q9CQA0-2]
DR Antibodypedia; 27112; 140 antibodies from 25 providers.
DR DNASU; 66570; -.
DR Ensembl; ENSMUST00000089155; ENSMUSP00000086558; ENSMUSG00000068101. [Q9CQA0-2]
DR Ensembl; ENSMUST00000089157; ENSMUSP00000086560; ENSMUSG00000068101. [Q9CQA0-1]
DR GeneID; 66570; -.
DR KEGG; mmu:66570; -.
DR UCSC; uc007wym.1; mouse. [Q9CQA0-1]
DR UCSC; uc007wyo.1; mouse. [Q9CQA0-2]
DR CTD; 79019; -.
DR MGI; MGI:1913820; Cenpm.
DR VEuPathDB; HostDB:ENSMUSG00000068101; -.
DR eggNOG; ENOG502S17M; Eukaryota.
DR GeneTree; ENSGT00390000017504; -.
DR HOGENOM; CLU_127179_0_0_1; -.
DR InParanoid; Q9CQA0; -.
DR OMA; FCELELE; -.
DR OrthoDB; 1551430at2759; -.
DR PhylomeDB; Q9CQA0; -.
DR TreeFam; TF328778; -.
DR Reactome; R-MMU-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR Reactome; R-MMU-2467813; Separation of Sister Chromatids.
DR Reactome; R-MMU-2500257; Resolution of Sister Chromatid Cohesion.
DR Reactome; R-MMU-5663220; RHO GTPases Activate Formins.
DR Reactome; R-MMU-606279; Deposition of new CENPA-containing nucleosomes at the centromere.
DR Reactome; R-MMU-68877; Mitotic Prometaphase.
DR Reactome; R-MMU-9648025; EML4 and NUDC in mitotic spindle formation.
DR BioGRID-ORCS; 66570; 34 hits in 75 CRISPR screens.
DR ChiTaRS; Cenpm; mouse.
DR PRO; PR:Q9CQA0; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q9CQA0; protein.
DR Bgee; ENSMUSG00000068101; Expressed in morula and 116 other tissues.
DR ExpressionAtlas; Q9CQA0; baseline and differential.
DR Genevisible; Q9CQA0; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR020987; Centromere_Cenp-M.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR34436; PTHR34436; 1.
DR Pfam; PF11111; CENP-M; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Centromere; Chromosome; Cytoplasm; Kinetochore;
KW Nucleus; Reference proteome.
FT CHAIN 1..180
FT /note="Centromere protein M"
FT /id="PRO_0000058225"
FT VAR_SEQ 104..180
FT /note="AGQESHCSVHQNTVIKLAHTYRSPLLLCDLQVESFRAAMARRLVRILQICAG
FT HVPGVSALNLMSLLRSPENPPSKEL -> GGKLSCSHGAASGAHSADLRWPCARRLRAE
FT PDVLAAEPREPPIQGAVSITALHTFAASSKSSHCW (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_020440"
FT CONFLICT 13
FT /note="L -> M (in Ref. 2; BAE40163)"
FT /evidence="ECO:0000305"
FT CONFLICT 47
FT /note="H -> N (in Ref. 2; BAE40163)"
FT /evidence="ECO:0000305"
FT CONFLICT 129
FT /note="L -> F (in Ref. 3; AAH09160)"
FT /evidence="ECO:0000305"
FT CONFLICT 134
FT /note="Q -> QK (in Ref. 3; AAH09160)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 180 AA; 20039 MW; AA6396B75269346C CRC64;
MSVLRSMDKL PDLNRATVLL VSTEDALLQQ LAESMLKDDC ASELRVHLAN SLPLPSNVNR
PRIDLIVFVI NLHSKYSLQK VEEFLQHVDS SFFLGKVCFL VTGAGQESHC SVHQNTVIKL
AHTYRSPLLL CDLQVESFRA AMARRLVRIL QICAGHVPGV SALNLMSLLR SPENPPSKEL
