ID   CENPN_BOVIN             Reviewed;         339 AA.
AC   Q32LL9;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Centromere protein N;
DE            Short=CENP-N;
GN   Name=CENPN;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the CENPA-NAC (nucleosome-associated) complex, a
CC       complex that plays a central role in assembly of kinetochore proteins,
CC       mitotic progression and chromosome segregation. The CENPA-NAC complex
CC       recruits the CENPA-CAD (nucleosome distal) complex and may be involved
CC       in incorporation of newly synthesized CENPA into centromeres. CENPN is
CC       the first protein to bind specifically to CENPA nucleosomes and the
CC       direct binding of CENPA nucleosomes by CENPN is required for centromere
CC       assembly. Required for chromosome congression and efficiently align the
CC       chromosomes on a metaphase plate. {ECO:0000250|UniProtKB:Q96H22}.
CC   -!- SUBUNIT: Component of the CENPA-NAC complex, at least composed of
CC       CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC
CC       complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK,
CC       CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts directly with
CC       CENPA. Identified in a centromere complex containing histones H2A, H2B
CC       and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H,
CC       SSRP1 and RSF1. {ECO:0000250|UniProtKB:Q96H22}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere
CC       {ECO:0000250}. Chromosome, centromere, kinetochore {ECO:0000250}.
CC       Note=Localizes exclusively in the kinetochore domain of centromeres.
CC       Kinetochore-bound levels decrease when cells enter mitosis and increase
CC       again when cells exit mitosis. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CENP-N/CHL4 family. {ECO:0000305}.
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DR   EMBL; BC109516; AAI09517.1; -; mRNA.
DR   RefSeq; NP_001033188.1; NM_001038099.2.
DR   AlphaFoldDB; Q32LL9; -.
DR   SMR; Q32LL9; -.
DR   STRING; 9913.ENSBTAP00000015452; -.
DR   PaxDb; Q32LL9; -.
DR   PRIDE; Q32LL9; -.
DR   GeneID; 513551; -.
DR   KEGG; bta:513551; -.
DR   CTD; 55839; -.
DR   eggNOG; ENOG502QSE8; Eukaryota.
DR   InParanoid; Q32LL9; -.
DR   OrthoDB; 1145159at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR   GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR   GO; GO:0007059; P:chromosome segregation; IEA:InterPro.
DR   GO; GO:0051382; P:kinetochore assembly; IEA:InterPro.
DR   InterPro; IPR007902; Chl4/mis15/CENP-N.
DR   Pfam; PF05238; CENP-N; 1.
PE   2: Evidence at transcript level;
KW   Centromere; Chromosome; Kinetochore; Nucleus; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN           1..339
FT                   /note="Centromere protein N"
FT                   /id="PRO_0000249493"
FT   MOD_RES         226
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96H22"
FT   MOD_RES         235
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96H22"
SQ   SEQUENCE   339 AA;  39411 MW;  0DC10E6B1E62D3F2 CRC64;
     MDETLAEFFR RTILKIPMTE MMTILKTWNF MSENQLQTVN FRQRKESIVQ DLVLLCEENH
     ASLNDAAHLD IIYTQFHRHQ KIWDVFQMSK APGDDIDLFD MEQFKSSFKK ILQRALKNVT
     VSFRDAEENS VWIRIAWGTQ YKKPNQYKPA YVVYYSQTPY AFTSSSRLKS NLPLLGQALT
     VASKHHQIVK MDLRSRYLDS LKAIVFKQYN QSFETHNCTT SLQEGSLGLD INMDSRIIHE
     NKVEKERVQR VTQEIFGDYP QPRLEFAQYK LETKFKSDLN GGILAEREEP LRCLVKFSSP
     HLLEALKSLA PAGIADAPLS PLLTCIPNKG KNYFKIRDK