CENPN_SCHPO
ID CENPN_SCHPO Reviewed; 409 AA.
AC Q9C0W0; A1IU53; O13613;
DT 01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 23-MAR-2010, sequence version 3.
DT 25-MAY-2022, entry version 124.
DE RecName: Full=Inner kinetochore subunit mis15;
DE AltName: Full=CENP-N homolog;
DE AltName: Full=Constitutive centromere-associated network protein mis15;
DE AltName: Full=Sim4 complex subunit mis15;
GN Name=mis15; ORFNames=pi022, SPBP22H7.09c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=10620777;
RX DOI=10.1002/(sici)1097-0061(20000115)16:1<71::aid-yea505>3.0.co;2-5;
RA Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K.,
RA Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y.,
RA Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M.;
RT "A 38 kb segment containing the cdc2 gene from the left arm of fission
RT yeast chromosome II: sequence analysis and characterization of the genomic
RT DNA and cDNAs encoded on the segment.";
RL Yeast 16:71-80(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP FUNCTION, INTERACTION WITH MIS6 AND MIS17, SUBCELLULAR LOCATION, AND
RP REVISION OF GENE MODEL.
RX PubMed=15369671; DOI=10.1016/j.cell.2004.09.002;
RA Hayashi T., Fujita Y., Iwasaki O., Adachi Y., Takahashi K., Yanagida M.;
RT "Mis16 and Mis18 are required for CENP-A loading and histone deacetylation
RT at centromeres.";
RL Cell 118:715-729(2004).
RN [4]
RP FUNCTION, AND IDENTIFICATION IN THE SIM4 COMPLEX.
RX PubMed=16079914; DOI=10.1038/sj.emboj.7600762;
RA Liu X., McLeod I., Anderson S., Yates J.R. III, He X.;
RT "Molecular analysis of kinetochore architecture in fission yeast.";
RL EMBO J. 24:2919-2930(2005).
RN [5]
RP FUNCTION.
RX PubMed=15897182; DOI=10.1098/rstb.2004.1614;
RA Takahashi K., Takayama Y., Masuda F., Kobayashi Y., Saitoh S.;
RT "Two distinct pathways responsible for the loading of CENP-A to centromeres
RT in the fission yeast cell cycle.";
RL Philos. Trans. R. Soc. Lond., B, Biol. Sci. 360:595-606(2005).
RN [6]
RP FUNCTION, AND IDENTIFICATION IN THE SIM4 COMPLEX.
RX PubMed=16855021; DOI=10.1091/mbc.e06-04-0264;
RA Kerres A., Jakopec V., Beuter C., Karig I., Pohlmann J., Pidoux A.,
RA Allshire R., Fleig U.;
RT "Fta2, an essential fission yeast kinetochore component, interacts closely
RT with the conserved Mal2 protein.";
RL Mol. Biol. Cell 17:4167-4178(2006).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=17035632; DOI=10.1091/mbc.e06-05-0388;
RA Hayashi A., Asakawa H., Haraguchi T., Hiraoka Y.;
RT "Reconstruction of the kinetochore during meiosis in fission yeast
RT Schizosaccharomyces pombe.";
RL Mol. Biol. Cell 17:5173-5184(2006).
RN [8]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [9]
RP IDENTIFICATION IN THE SIM4 COMPLEX.
RX PubMed=21445296; DOI=10.1371/journal.pone.0017761;
RA Shiroiwa Y., Hayashi T., Fujita Y., Villar-Briones A., Ikai N., Takeda K.,
RA Ebe M., Yanagida M.;
RT "Mis17 is a regulatory module of the Mis6-Mal2-Sim4 centromere complex that
RT is required for the recruitment of CenH3/CENP-A in fission yeast.";
RL PLoS ONE 6:E17761-E17761(2011).
CC -!- FUNCTION: Component of the kinetochore, a multiprotein complex that
CC assembles on centromeric DNA and attaches chromosomes to spindle
CC microtubules, mediating chromosome segregation and sister chromatid
CC segregation during meiosis and mitosis. Component of the inner
CC kinetochore constitutive centromere-associated network (CCAN), which
CC serves as a structural platform for outer kinetochore assembly
CC (PubMed:16079914, PubMed:16855021). Has a role in the formation and/or
CC maintenance of specialized chromatin at the centromere
CC (PubMed:15369671, PubMed:15897182). {ECO:0000269|PubMed:15369671,
CC ECO:0000269|PubMed:15897182, ECO:0000269|PubMed:16079914,
CC ECO:0000269|PubMed:16855021}.
CC -!- SUBUNIT: Component of the inner kinetochore constitutive centromere-
CC associated network (CCAN) (also known as central kinetochore Sim4
CC complex in fission yeast), which is composed of at least cnl2, cnp3,
CC cnp20, fta1, fta2, fta3, fta4, fta6, fta7, mal2, mhf1, mhf2, mis6,
CC mis15, mis17, sim4 and wip1 (PubMed:16079914, PubMed:16855021,
CC PubMed:21445296). Interacts with mis6 and mis17 (PubMed:15369671).
CC {ECO:0000269|PubMed:15369671, ECO:0000269|PubMed:16079914,
CC ECO:0000269|PubMed:16855021, ECO:0000269|PubMed:21445296}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome.
CC -!- SIMILARITY: Belongs to the CENP-N/CHL4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA21401.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAA21401.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB004535; BAA21401.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CU329671; CAM11335.2; -; Genomic_DNA.
DR RefSeq; XP_001713131.2; XM_001713079.2.
DR AlphaFoldDB; Q9C0W0; -.
DR SMR; Q9C0W0; -.
DR BioGRID; 277833; 11.
DR IntAct; Q9C0W0; 2.
DR STRING; 4896.SPBP22H7.09c.1; -.
DR MaxQB; Q9C0W0; -.
DR PaxDb; Q9C0W0; -.
DR EnsemblFungi; SPBP22H7.09c.1; SPBP22H7.09c.1:pep; SPBP22H7.09c.
DR PomBase; SPBP22H7.09c; mis15.
DR VEuPathDB; FungiDB:SPBP22H7.09c; -.
DR eggNOG; ENOG502QVRZ; Eukaryota.
DR HOGENOM; CLU_714015_0_0_1; -.
DR InParanoid; Q9C0W0; -.
DR OMA; DWRHGIT; -.
DR PhylomeDB; Q9C0W0; -.
DR PRO; PR:Q9C0W0; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0000779; C:condensed chromosome, centromeric region; IDA:PomBase.
DR GO; GO:0000776; C:kinetochore; IDA:PomBase.
DR GO; GO:0031511; C:Mis6-Sim4 complex; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0034080; P:CENP-A containing chromatin assembly; IMP:PomBase.
DR GO; GO:0051382; P:kinetochore assembly; IEA:InterPro.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IMP:PomBase.
DR InterPro; IPR007902; Chl4/mis15/CENP-N.
DR Pfam; PF05238; CENP-N; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Chromosome; Mitosis; Nucleus;
KW Reference proteome.
FT CHAIN 1..409
FT /note="Inner kinetochore subunit mis15"
FT /id="PRO_0000116774"
SQ SEQUENCE 409 AA; 47394 MW; 60938B407FB66897 CRC64;
MRTELSSNIR IKHDKKIQKL LNRFPRDFLV KLCVEWIQKQ TYPPNAKDIN LEDMLDDEEW
NPEAFYKNVP KSMLKRSIIH RMLVYDWPNG FYLGQIAQLE ILALAHGFVS MRWTASKVHH
SAEKTVLPNP LVFLELLKSE LESIFVYHTY ISRHETLPIT FIRLVLWDSK RPTALHTYPS
SKQIFYLGLM DDSDVLLHNI FLRNDVCHSL FLQCLSRLLY RLKAGSALRP IDLVSKNLTT
FCTNVGVNKE ANALGAWQIY AKNLVDRSPL DTRPILSDDN SSLIADSTQN CEKHREMAIQ
RRFGDTHSQV LDKLLITLDH DYIEKSTEKD KVLEENVESY NPTEQRPLVV MQLRGQHILE
GLKDICRQDA LDPFTMPSYL TGETGLSILY VRDGLVLQRQ ESLGQQEIN
