CENPO_HUMAN
ID CENPO_HUMAN Reviewed; 300 AA.
AC Q9BU64; B2RDC0; D6W536; Q53T55; Q96JV3;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Centromere protein O;
DE Short=CENP-O;
DE AltName: Full=Interphase centromere complex protein 36;
GN Name=CENPO; Synonyms=ICEN36, MCM21R;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANT
RP ARG-34.
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16932742; DOI=10.1038/sj.emboj.7601293;
RA McAinsh A.D., Meraldi P., Draviam V.M., Toso A., Sorger P.K.;
RT "The human kinetochore proteins Nnf1R and Mcm21R are required for accurate
RT chromosome segregation.";
RL EMBO J. 25:4033-4049(2006).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16716197; DOI=10.1111/j.1365-2443.2006.00969.x;
RA Izuta H., Ikeno M., Suzuki N., Tomonaga T., Nozaki N., Obuse C., Kisu Y.,
RA Goshima N., Nomura F., Nomura N., Yoda K.;
RT "Comprehensive analysis of the ICEN (Interphase Centromere Complex)
RT components enriched in the CENP-A chromatin of human cells.";
RL Genes Cells 11:673-684(2006).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH
RP CENPH; CENPI; CENPK; CENPN; CENPP; CENPQ; CENPR AND CENPU, FUNCTION, AND
RP SUBCELLULAR LOCATION.
RX PubMed=16622420; DOI=10.1038/ncb1396;
RA Okada M., Cheeseman I.M., Hori T., Okawa K., McLeod I.X., Yates J.R. III,
RA Desai A., Fukagawa T.;
RT "The CENP-H-I complex is required for the efficient incorporation of newly
RT synthesized CENP-A into centromeres.";
RL Nat. Cell Biol. 8:446-457(2006).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE CENPA-CAD
RP COMPLEX WITH CENPI; CENPK; CENPL; CENPP; CENPQ; CENPR AND CENPS.
RX PubMed=16622419; DOI=10.1038/ncb1397;
RA Foltz D.R., Jansen L.E.T., Black B.E., Bailey A.O., Yates J.R. III,
RA Cleveland D.W.;
RT "The human CENP-A centromeric nucleosome-associated complex.";
RL Nat. Cell Biol. 8:458-469(2006).
RN [9]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=18007590; DOI=10.1038/sj.emboj.7601927;
RA McClelland S.E., Borusu S., Amaro A.C., Winter J.R., Belwal M.,
RA McAinsh A.D., Meraldi P.;
RT "The CENP-A NAC/CAD kinetochore complex controls chromosome congression and
RT spindle bipolarity.";
RL EMBO J. 26:5033-5047(2007).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-35, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Component of the CENPA-CAD (nucleosome distal) complex, a
CC complex recruited to centromeres which is involved in assembly of
CC kinetochore proteins, mitotic progression and chromosome segregation.
CC May be involved in incorporation of newly synthesized CENPA into
CC centromeres via its interaction with the CENPA-NAC complex. Modulates
CC the kinetochore-bound levels of NDC80 complex.
CC {ECO:0000269|PubMed:16622420, ECO:0000269|PubMed:16716197,
CC ECO:0000269|PubMed:16932742, ECO:0000269|PubMed:18007590}.
CC -!- SUBUNIT: Component of the CENPA-CAD complex, composed of CENPI, CENPK,
CC CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex
CC interacts with the CENPA-NAC complex, at least composed of CENPA,
CC CENPC, CENPH, CENPM, CENPN, CENPT and CENPU.
CC {ECO:0000269|PubMed:16622419, ECO:0000269|PubMed:16622420}.
CC -!- INTERACTION:
CC Q9BU64; Q6IPU0: CENPP; NbExp=11; IntAct=EBI-745954, EBI-10250303;
CC Q9BU64; Q96L14: CEP170P1; NbExp=5; IntAct=EBI-745954, EBI-743488;
CC Q9BU64; Q9UHG0: DCDC2; NbExp=3; IntAct=EBI-745954, EBI-10303987;
CC Q9BU64; Q6FG41: FOS; NbExp=3; IntAct=EBI-745954, EBI-10198738;
CC Q9BU64; P53539: FOSB; NbExp=3; IntAct=EBI-745954, EBI-2806743;
CC Q9BU64; Q7Z6G3-2: NECAB2; NbExp=3; IntAct=EBI-745954, EBI-10172876;
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere. Chromosome,
CC centromere, kinetochore. Note=The CENPA-CAD complex is probably
CC recruited on centromeres by the CENPA-NAC complex.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9BU64-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9BU64-2; Sequence=VSP_020446;
CC -!- SIMILARITY: Belongs to the CENP-O/MCM21 family. {ECO:0000305}.
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DR EMBL; AK027859; BAB55414.1; -; mRNA.
DR EMBL; AK315483; BAG37867.1; -; mRNA.
DR EMBL; AC012073; AAY14786.1; -; Genomic_DNA.
DR EMBL; AC013459; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471053; EAX00742.1; -; Genomic_DNA.
DR EMBL; CH471053; EAX00743.1; -; Genomic_DNA.
DR EMBL; BC002870; AAH02870.1; -; mRNA.
DR CCDS; CCDS1714.1; -. [Q9BU64-1]
DR CCDS; CCDS56113.1; -. [Q9BU64-2]
DR RefSeq; NP_001186732.1; NM_001199803.2. [Q9BU64-2]
DR RefSeq; NP_001309030.1; NM_001322101.1. [Q9BU64-1]
DR RefSeq; NP_077298.1; NM_024322.3. [Q9BU64-1]
DR AlphaFoldDB; Q9BU64; -.
DR SMR; Q9BU64; -.
DR BioGRID; 122589; 49.
DR ComplexPortal; CPX-5646; Kinetochore CCAN complex.
DR CORUM; Q9BU64; -.
DR IntAct; Q9BU64; 33.
DR MINT; Q9BU64; -.
DR STRING; 9606.ENSP00000370214; -.
DR iPTMnet; Q9BU64; -.
DR PhosphoSitePlus; Q9BU64; -.
DR BioMuta; CENPO; -.
DR DMDM; 74733185; -.
DR EPD; Q9BU64; -.
DR jPOST; Q9BU64; -.
DR MassIVE; Q9BU64; -.
DR MaxQB; Q9BU64; -.
DR PaxDb; Q9BU64; -.
DR PeptideAtlas; Q9BU64; -.
DR PRIDE; Q9BU64; -.
DR ProteomicsDB; 79055; -. [Q9BU64-1]
DR ProteomicsDB; 79056; -. [Q9BU64-2]
DR Antibodypedia; 27556; 168 antibodies from 30 providers.
DR DNASU; 79172; -.
DR Ensembl; ENST00000260662.2; ENSP00000260662.1; ENSG00000138092.11. [Q9BU64-1]
DR Ensembl; ENST00000380834.7; ENSP00000370214.2; ENSG00000138092.11. [Q9BU64-1]
DR Ensembl; ENST00000473706.5; ENSP00000417787.1; ENSG00000138092.11. [Q9BU64-2]
DR GeneID; 79172; -.
DR KEGG; hsa:79172; -.
DR MANE-Select; ENST00000380834.7; ENSP00000370214.2; NM_001322101.2; NP_001309030.1.
DR UCSC; uc002rfp.3; human. [Q9BU64-1]
DR CTD; 79172; -.
DR DisGeNET; 79172; -.
DR GeneCards; CENPO; -.
DR HGNC; HGNC:28152; CENPO.
DR HPA; ENSG00000138092; Low tissue specificity.
DR MIM; 611504; gene.
DR neXtProt; NX_Q9BU64; -.
DR OpenTargets; ENSG00000138092; -.
DR PharmGKB; PA145149197; -.
DR VEuPathDB; HostDB:ENSG00000138092; -.
DR eggNOG; ENOG502RY72; Eukaryota.
DR GeneTree; ENSGT00390000016702; -.
DR HOGENOM; CLU_079531_0_0_1; -.
DR InParanoid; Q9BU64; -.
DR OMA; FCISTAY; -.
DR OrthoDB; 954886at2759; -.
DR PhylomeDB; Q9BU64; -.
DR TreeFam; TF335524; -.
DR PathwayCommons; Q9BU64; -.
DR Reactome; R-HSA-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR Reactome; R-HSA-2467813; Separation of Sister Chromatids.
DR Reactome; R-HSA-2500257; Resolution of Sister Chromatid Cohesion.
DR Reactome; R-HSA-5663220; RHO GTPases Activate Formins.
DR Reactome; R-HSA-606279; Deposition of new CENPA-containing nucleosomes at the centromere.
DR Reactome; R-HSA-68877; Mitotic Prometaphase.
DR Reactome; R-HSA-9648025; EML4 and NUDC in mitotic spindle formation.
DR SignaLink; Q9BU64; -.
DR SIGNOR; Q9BU64; -.
DR BioGRID-ORCS; 79172; 105 hits in 1083 CRISPR screens.
DR ChiTaRS; CENPO; human.
DR GeneWiki; CENPO; -.
DR GenomeRNAi; 79172; -.
DR Pharos; Q9BU64; Tbio.
DR PRO; PR:Q9BU64; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q9BU64; protein.
DR Bgee; ENSG00000138092; Expressed in ventricular zone and 120 other tissues.
DR Genevisible; Q9BU64; HS.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0031511; C:Mis6-Sim4 complex; IBA:GO_Central.
DR GO; GO:0016604; C:nuclear body; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0034508; P:centromere complex assembly; IEA:InterPro.
DR InterPro; IPR018464; CENP-O.
DR PANTHER; PTHR14582; PTHR14582; 1.
DR Pfam; PF09496; CENP-O; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Centromere; Chromosome; Coiled coil; Kinetochore;
KW Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..300
FT /note="Centromere protein O"
FT /id="PRO_0000249502"
FT COILED 18..42
FT /evidence="ECO:0000255"
FT COILED 83..109
FT /evidence="ECO:0000255"
FT MOD_RES 35
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 1..15
FT /note="MEQANPLRPDGESKG -> MAGILASGL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_020446"
FT VARIANT 34
FT /note="Q -> R (in dbSNP:rs1550116)"
FT /evidence="ECO:0000269|PubMed:14702039"
FT /id="VAR_027420"
FT CONFLICT 235
FT /note="A -> V (in Ref. 1; BAB55414)"
FT /evidence="ECO:0000305"
FT CONFLICT 269
FT /note="A -> V (in Ref. 1; BAB55414)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 300 AA; 33786 MW; 05A89E12C6847D81 CRC64;
MEQANPLRPD GESKGGVLAH LERLETQVSR SRKQSEELQS VQAQEGALGT KIHKLRRLRD
ELRAVVRHRR ASVKACIANV EPNQTVEINE QEALEEKLEN VKAILQAYHF TGLSGKLTSR
GVCVCISTAF EGNLLDSYFV DLVIQKPLRI HHHSVPVFIP LEEIAAKYLQ TNIQHFLFSL
CEYLNAYSGR KYQADRLQSD FAALLTGPLQ RNPLCNLLSF TYKLDPGGQS FPFCARLLYK
DLTATLPTDV TVTCQGVEVL STSWEEQRAS HETLFCTKPL HQVFASFTRK GEKLDMSLVS
