CENPO_MOUSE
ID CENPO_MOUSE Reviewed; 298 AA.
AC Q8K015; Q8R587; Q9CYY6;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Centromere protein O;
DE Short=CENP-O;
GN Name=Cenpo; Synonyms=Mcm21r;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Forelimb, and Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=Czech II; TISSUE=Eye, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the CENPA-CAD (nucleosome distal) complex, a
CC complex recruited to centromeres which is involved in assembly of
CC kinetochore proteins, mitotic progression and chromosome segregation.
CC May be involved in incorporation of newly synthesized CENPA into
CC centromeres via its interaction with the CENPA-NAC complex. Modulates
CC the kinetochore-bound levels of NDC80 complex (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of the CENPA-CAD complex, composed of CENPI, CENPK,
CC CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex
CC interacts with the CENPA-NAC complex, at least composed of CENPA,
CC CENPC, CENPH, CENPM, CENPN, CENPT and CENPU (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere
CC {ECO:0000250}. Chromosome, centromere, kinetochore {ECO:0000250}.
CC Note=Localizes exclusively in the centromeres. The CENPA-CAD complex is
CC probably recruited on centromeres by the CENPA-NAC complex (By
CC similarity). {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8K015-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8K015-2; Sequence=VSP_020447, VSP_020448;
CC -!- SIMILARITY: Belongs to the CENP-O/MCM21 family. {ECO:0000305}.
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DR EMBL; AK013198; BAB28707.1; -; mRNA.
DR EMBL; AK029226; BAC26354.1; -; mRNA.
DR EMBL; AK134266; BAE22073.1; -; mRNA.
DR EMBL; BC023148; AAH23148.1; -; mRNA.
DR EMBL; BC034399; AAH34399.1; -; mRNA.
DR CCDS; CCDS25788.1; -. [Q8K015-1]
DR RefSeq; NP_598807.2; NM_134046.5. [Q8K015-1]
DR RefSeq; XP_006515215.1; XM_006515152.3. [Q8K015-2]
DR RefSeq; XP_011242186.1; XM_011243884.2. [Q8K015-2]
DR AlphaFoldDB; Q8K015; -.
DR SMR; Q8K015; -.
DR BioGRID; 206626; 3.
DR ComplexPortal; CPX-5704; Kinetochore CCAN complex.
DR CORUM; Q8K015; -.
DR IntAct; Q8K015; 1.
DR MINT; Q8K015; -.
DR STRING; 10090.ENSMUSP00000119136; -.
DR iPTMnet; Q8K015; -.
DR PhosphoSitePlus; Q8K015; -.
DR EPD; Q8K015; -.
DR MaxQB; Q8K015; -.
DR PaxDb; Q8K015; -.
DR PeptideAtlas; Q8K015; -.
DR PRIDE; Q8K015; -.
DR ProteomicsDB; 281374; -. [Q8K015-1]
DR ProteomicsDB; 281375; -. [Q8K015-2]
DR Antibodypedia; 27556; 168 antibodies from 30 providers.
DR DNASU; 52504; -.
DR Ensembl; ENSMUST00000111169; ENSMUSP00000106799; ENSMUSG00000020652. [Q8K015-2]
DR Ensembl; ENSMUST00000140975; ENSMUSP00000119136; ENSMUSG00000020652. [Q8K015-1]
DR GeneID; 52504; -.
DR KEGG; mmu:52504; -.
DR UCSC; uc007mxn.1; mouse. [Q8K015-1]
DR CTD; 79172; -.
DR MGI; MGI:1923800; Cenpo.
DR VEuPathDB; HostDB:ENSMUSG00000020652; -.
DR eggNOG; ENOG502RY72; Eukaryota.
DR GeneTree; ENSGT00390000016702; -.
DR InParanoid; Q8K015; -.
DR OMA; FCISTAY; -.
DR OrthoDB; 954886at2759; -.
DR PhylomeDB; Q8K015; -.
DR TreeFam; TF335524; -.
DR Reactome; R-MMU-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR Reactome; R-MMU-2467813; Separation of Sister Chromatids.
DR Reactome; R-MMU-2500257; Resolution of Sister Chromatid Cohesion.
DR Reactome; R-MMU-5663220; RHO GTPases Activate Formins.
DR Reactome; R-MMU-606279; Deposition of new CENPA-containing nucleosomes at the centromere.
DR Reactome; R-MMU-68877; Mitotic Prometaphase.
DR Reactome; R-MMU-9648025; EML4 and NUDC in mitotic spindle formation.
DR BioGRID-ORCS; 52504; 28 hits in 75 CRISPR screens.
DR ChiTaRS; Cenpo; mouse.
DR PRO; PR:Q8K015; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q8K015; protein.
DR Bgee; ENSMUSG00000020652; Expressed in primary oocyte and 209 other tissues.
DR ExpressionAtlas; Q8K015; baseline and differential.
DR Genevisible; Q8K015; MM.
DR GO; GO:0031511; C:Mis6-Sim4 complex; IBA:GO_Central.
DR GO; GO:0016604; C:nuclear body; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0034508; P:centromere complex assembly; IEA:InterPro.
DR InterPro; IPR018464; CENP-O.
DR PANTHER; PTHR14582; PTHR14582; 1.
DR Pfam; PF09496; CENP-O; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Centromere; Chromosome; Coiled coil; Kinetochore;
KW Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..298
FT /note="Centromere protein O"
FT /id="PRO_0000249503"
FT REGION 29..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 39..74
FT /evidence="ECO:0000255"
FT MOD_RES 35
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BU64"
FT VAR_SEQ 197..205
FT /note="SDFCDVLTG -> NWTADSPSR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_020447"
FT VAR_SEQ 206..298
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_020448"
FT CONFLICT 30
FT /note="I -> V (in Ref. 2; AAH23148)"
FT /evidence="ECO:0000305"
FT CONFLICT 39
FT /note="A -> T (in Ref. 2; AAH23148)"
FT /evidence="ECO:0000305"
FT CONFLICT 177
FT /note="R -> S (in Ref. 2; AAH23148)"
FT /evidence="ECO:0000305"
FT CONFLICT 180
FT /note="E -> A (in Ref. 2; AAH23148)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 298 AA; 34154 MW; 6A616612463B31E9 CRC64;
MESANTLCPG RKCKGGVLAH LERLEAQTNI SNRKSEEPAV RKKESSLRTK IRELRQQRDK
LRAEVKQWGA RVKEPPAKED PSRTVISEQE VLEREWRNVD AILEAYRFTG LSGKLTSRGV
CMCISTAFEG NLLDSYFVDL VIEKPLRIHH HSVPVFIPLE KIAAAHLQTD VQRFLFRLWE
YLNAYAGRKY QADQLESDFC DVLTGPLQRN ALCNLLSFTY KVEQRCQTFS FSARLLYEDP
TAALPTNVTV TRPGVEASSP PWEEHRASHQ MLFRTKPLHK VFASFSKETE KLHLNLVS
