CENPO_SCHPO
ID CENPO_SCHPO Reviewed; 303 AA.
AC Q10290;
DT 06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=Inner kinetochore subunit mal2;
DE AltName: Full=CENP-O homolog;
DE AltName: Full=Constitutive centromere-associated network protein mal2;
DE AltName: Full=Sim4 complex subunit mal2;
GN Name=mal2; ORFNames=SPAC25B8.14;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=8887647; DOI=10.1128/mcb.16.11.6169;
RA Fleig U., Sen-Gupta M., Hegemann J.H.;
RT "Fission yeast mal2+ is required for chromosome segregation.";
RL Mol. Cell. Biol. 16:6169-6177(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP FUNCTION, INTERACTION WITH MIS12, AND SUBCELLULAR LOCATION.
RX PubMed=12242294; DOI=10.1128/mcb.22.20.7168-7183.2002;
RA Jin Q.-W., Pidoux A.L., Decker C., Allshire R.C., Fleig U.;
RT "The mal2p protein is an essential component of the fission yeast
RT centromere.";
RL Mol. Cell. Biol. 22:7168-7183(2002).
RN [4]
RP FUNCTION, AND IDENTIFICATION IN THE SIM4 COMPLEX.
RX PubMed=16079914; DOI=10.1038/sj.emboj.7600762;
RA Liu X., McLeod I., Anderson S., Yates J.R. III, He X.;
RT "Molecular analysis of kinetochore architecture in fission yeast.";
RL EMBO J. 24:2919-2930(2005).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Component of the kinetochore, a multiprotein complex that
CC assembles on centromeric DNA and attaches chromosomes to spindle
CC microtubules, mediating chromosome segregation and sister chromatid
CC segregation during meiosis and mitosis. Component of the inner
CC kinetochore COMA complex, which connects centromere-associated proteins
CC and the outer kinetochore. COMA interacts with other inner kinetochore
CC proteins to form the inner kinetochore constitutive centromere-
CC associated network (CCAN), which serves as a structural platform for
CC outer kinetochore assembly. {ECO:0000269|PubMed:12242294,
CC ECO:0000269|PubMed:16079914, ECO:0000269|PubMed:8887647}.
CC -!- SUBUNIT: Component of the heterotetrameric kinetochore subcomplex COMA,
CC which consists of fta2, fta7, mal2 and mis17 (By similarity). The COMA
CC subcomplex is part of a larger constitutive centromere-associated
CC network (CCAN) (also known as central kinetochore Sim4 complex in
CC fission yeast), which is composed of at least cnl2, cnp3, cnp20, fta1,
CC fta2, fta3, fta4, fta6, fta7, mal2, mhf1, mhf2, mis6, mis15, mis17,
CC sim4 and wip1 (PubMed:12242294, PubMed:16079914).
CC {ECO:0000250|UniProtKB:Q02732, ECO:0000269|PubMed:12242294,
CC ECO:0000269|PubMed:16079914}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere, kinetochore.
CC Chromosome, centromere.
CC -!- SIMILARITY: Belongs to the CENP-O/MCM21 family. {ECO:0000305}.
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DR EMBL; X92349; CAA63106.1; -; Genomic_DNA.
DR EMBL; CU329670; CAB61780.1; -; Genomic_DNA.
DR PIR; T43411; T43411.
DR RefSeq; NP_594474.1; NM_001019903.2.
DR AlphaFoldDB; Q10290; -.
DR SMR; Q10290; -.
DR BioGRID; 279187; 25.
DR IntAct; Q10290; 1.
DR STRING; 4896.SPAC25B8.14.1; -.
DR MaxQB; Q10290; -.
DR PaxDb; Q10290; -.
DR EnsemblFungi; SPAC25B8.14.1; SPAC25B8.14.1:pep; SPAC25B8.14.
DR GeneID; 2542737; -.
DR KEGG; spo:SPAC25B8.14; -.
DR PomBase; SPAC25B8.14; mal2.
DR VEuPathDB; FungiDB:SPAC25B8.14; -.
DR HOGENOM; CLU_966948_0_0_1; -.
DR OMA; HYIIFRV; -.
DR PRO; PR:Q10290; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0000779; C:condensed chromosome, centromeric region; IDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0000776; C:kinetochore; IDA:PomBase.
DR GO; GO:0031511; C:Mis6-Sim4 complex; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; IDA:PomBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0034508; P:centromere complex assembly; IEA:InterPro.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IC:PomBase.
DR InterPro; IPR018464; CENP-O.
DR PANTHER; PTHR14582; PTHR14582; 1.
DR Pfam; PF09496; CENP-O; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Kinetochore; Mitosis;
KW Nucleus; Reference proteome.
FT CHAIN 1..303
FT /note="Inner kinetochore subunit mal2"
FT /id="PRO_0000084556"
SQ SEQUENCE 303 AA; 34515 MW; 41C029B4C86DCF09 CRC64;
MDDEEGNATL IDEISVLEAR RDVLLDELKS LDNATLSDLV QKPELSKNLS VNNEFLKETP
LNQPALHYDR LSGISFFQPN DPEELSRKTL LVKNKKTGAI ETAPSIPLLG VRFDIMLNPV
NIGFTNLSEN PISSASGMDE ELEGGNRFDV PYYIIFRVFH DSFALYKYTI PSFLNIQEWA
DEYLTGKNPE RFRIFLWKVD KLLTAYICRK NALLQINKSL TIDGTNISAN LSCTHLVIQI
PKVIQATLVC SSESTRVVKS RIYQYSDENW KRDHRLELSL LDNKRWVNIL VSHLTAPESH
ASL
