CENPQ_HUMAN
ID CENPQ_HUMAN Reviewed; 268 AA.
AC Q7L2Z9; A8KAF1; Q6IN61; Q9NVS5;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Centromere protein Q;
DE Short=CENP-Q;
GN Name=CENPQ; Synonyms=C6orf139;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Stomach, and Teratocarcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH
RP CENPH; CENPI; CENPK; CENPN; CENPO; CENPP; CENPR AND CENPU, FUNCTION, AND
RP SUBCELLULAR LOCATION.
RX PubMed=16622420; DOI=10.1038/ncb1396;
RA Okada M., Cheeseman I.M., Hori T., Okawa K., McLeod I.X., Yates J.R. III,
RA Desai A., Fukagawa T.;
RT "The CENP-H-I complex is required for the efficient incorporation of newly
RT synthesized CENP-A into centromeres.";
RL Nat. Cell Biol. 8:446-457(2006).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE CENPA-CAD
RP COMPLEX WITH CENPI; CENPK; CENPL; CENPO; CENPP; CENPR AND CENPS.
RX PubMed=16622419; DOI=10.1038/ncb1397;
RA Foltz D.R., Jansen L.E.T., Black B.E., Bailey A.O., Yates J.R. III,
RA Cleveland D.W.;
RT "The human CENP-A centromeric nucleosome-associated complex.";
RL Nat. Cell Biol. 8:458-469(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31 AND SER-249, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-249, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [9]
RP FUNCTION, PHOSPHORYLATION AT SER-50, AND MUTAGENESIS OF SER-50.
RX PubMed=25395579; DOI=10.1242/jcs.163659;
RA Bancroft J., Auckland P., Samora C.P., McAinsh A.D.;
RT "Chromosome congression is promoted by CENP-Q- and CENP-E-dependent
RT pathways.";
RL J. Cell Sci. 128:171-184(2015).
CC -!- FUNCTION: Component of the CENPA-CAD (nucleosome distal) complex, a
CC complex recruited to centromeres which is involved in assembly of
CC kinetochore proteins, mitotic progression and chromosome segregation.
CC May be involved in incorporation of newly synthesized CENPA into
CC centromeres via its interaction with the CENPA-NAC complex
CC (PubMed:16622420). Plays an important role in chromosome congression
CC and in the recruitment of CENP-O complex (which comprises CENPO, CENPP,
CC CENPQ and CENPU), CENPE and PLK1 to the kinetochores (PubMed:25395579).
CC {ECO:0000269|PubMed:16622420, ECO:0000269|PubMed:25395579}.
CC -!- SUBUNIT: Component of the CENPA-CAD complex, composed of CENPI, CENPK,
CC CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex
CC interacts with the CENPA-NAC complex, at least composed of CENPA,
CC CENPC, CENPH, CENPM, CENPN, CENPT and CENPU.
CC {ECO:0000269|PubMed:16622419, ECO:0000269|PubMed:16622420}.
CC -!- INTERACTION:
CC Q7L2Z9; Q4LEZ3: AARD; NbExp=3; IntAct=EBI-2350265, EBI-5463075;
CC Q7L2Z9; Q9NYB9-2: ABI2; NbExp=3; IntAct=EBI-2350265, EBI-11096309;
CC Q7L2Z9; Q9P2A4: ABI3; NbExp=3; IntAct=EBI-2350265, EBI-742038;
CC Q7L2Z9; P15336: ATF2; NbExp=3; IntAct=EBI-2350265, EBI-1170906;
CC Q7L2Z9; Q16204: CCDC6; NbExp=3; IntAct=EBI-2350265, EBI-1045350;
CC Q7L2Z9; Q9Y6C2-2: EMILIN1; NbExp=3; IntAct=EBI-2350265, EBI-11748557;
CC Q7L2Z9; Q8WUI4-6: HDAC7; NbExp=3; IntAct=EBI-2350265, EBI-12094670;
CC Q7L2Z9; Q86YM7: HOMER1; NbExp=4; IntAct=EBI-2350265, EBI-746815;
CC Q7L2Z9; Q9BRV8: SIKE1; NbExp=3; IntAct=EBI-2350265, EBI-1773646;
CC Q7L2Z9; O60749: SNX2; NbExp=3; IntAct=EBI-2350265, EBI-1046690;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16622420}.
CC Chromosome, centromere {ECO:0000269|PubMed:16622420}. Note=Localizes
CC exclusively in the centromeres. The CENPA-CAD complex is probably
CC recruited on centromeres by the CENPA-NAC complex.
CC -!- PTM: Phosphorylation at Ser-50 is essential for CENPE recruitment to
CC kinetochores and orderly chromosome congression.
CC {ECO:0000269|PubMed:25395579}.
CC -!- SIMILARITY: Belongs to the CENP-Q/OKP1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA91672.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK001407; BAA91672.1; ALT_INIT; mRNA.
DR EMBL; AK293016; BAF85705.1; -; mRNA.
DR EMBL; AL590244; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL590668; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC016279; AAH16279.2; -; mRNA.
DR CCDS; CCDS4925.1; -.
DR RefSeq; NP_060602.2; NM_018132.3.
DR RefSeq; XP_005249262.1; XM_005249205.1.
DR AlphaFoldDB; Q7L2Z9; -.
DR SMR; Q7L2Z9; -.
DR BioGRID; 120466; 102.
DR ComplexPortal; CPX-5646; Kinetochore CCAN complex.
DR CORUM; Q7L2Z9; -.
DR IntAct; Q7L2Z9; 44.
DR MINT; Q7L2Z9; -.
DR STRING; 9606.ENSP00000337289; -.
DR iPTMnet; Q7L2Z9; -.
DR PhosphoSitePlus; Q7L2Z9; -.
DR BioMuta; CENPQ; -.
DR DMDM; 68565184; -.
DR EPD; Q7L2Z9; -.
DR jPOST; Q7L2Z9; -.
DR MassIVE; Q7L2Z9; -.
DR MaxQB; Q7L2Z9; -.
DR PaxDb; Q7L2Z9; -.
DR PeptideAtlas; Q7L2Z9; -.
DR PRIDE; Q7L2Z9; -.
DR ProteomicsDB; 68768; -.
DR Antibodypedia; 30817; 157 antibodies from 25 providers.
DR DNASU; 55166; -.
DR Ensembl; ENST00000335783.4; ENSP00000337289.2; ENSG00000031691.7.
DR GeneID; 55166; -.
DR KEGG; hsa:55166; -.
DR MANE-Select; ENST00000335783.4; ENSP00000337289.2; NM_018132.4; NP_060602.2.
DR UCSC; uc003ozh.2; human.
DR CTD; 55166; -.
DR DisGeNET; 55166; -.
DR GeneCards; CENPQ; -.
DR HGNC; HGNC:21347; CENPQ.
DR HPA; ENSG00000031691; Low tissue specificity.
DR MIM; 611506; gene.
DR neXtProt; NX_Q7L2Z9; -.
DR OpenTargets; ENSG00000031691; -.
DR PharmGKB; PA134973276; -.
DR VEuPathDB; HostDB:ENSG00000031691; -.
DR eggNOG; ENOG502S34R; Eukaryota.
DR GeneTree; ENSGT00390000005599; -.
DR HOGENOM; CLU_085740_0_0_1; -.
DR InParanoid; Q7L2Z9; -.
DR OMA; VFIEEAY; -.
DR OrthoDB; 1159538at2759; -.
DR PhylomeDB; Q7L2Z9; -.
DR TreeFam; TF336238; -.
DR PathwayCommons; Q7L2Z9; -.
DR Reactome; R-HSA-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR Reactome; R-HSA-2467813; Separation of Sister Chromatids.
DR Reactome; R-HSA-2500257; Resolution of Sister Chromatid Cohesion.
DR Reactome; R-HSA-5663220; RHO GTPases Activate Formins.
DR Reactome; R-HSA-606279; Deposition of new CENPA-containing nucleosomes at the centromere.
DR Reactome; R-HSA-68877; Mitotic Prometaphase.
DR Reactome; R-HSA-9648025; EML4 and NUDC in mitotic spindle formation.
DR SignaLink; Q7L2Z9; -.
DR SIGNOR; Q7L2Z9; -.
DR BioGRID-ORCS; 55166; 69 hits in 1077 CRISPR screens.
DR ChiTaRS; CENPQ; human.
DR GenomeRNAi; 55166; -.
DR Pharos; Q7L2Z9; Tbio.
DR PRO; PR:Q7L2Z9; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q7L2Z9; protein.
DR Bgee; ENSG00000031691; Expressed in ventricular zone and 128 other tissues.
DR Genevisible; Q7L2Z9; HS.
DR GO; GO:0015629; C:actin cytoskeleton; IDA:HPA.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0031511; C:Mis6-Sim4 complex; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0051310; P:metaphase plate congression; IMP:UniProtKB.
DR GO; GO:1905342; P:positive regulation of protein localization to kinetochore; IMP:UniProtKB.
DR InterPro; IPR025212; CAD_CENP-Q.
DR PANTHER; PTHR31345; PTHR31345; 1.
DR Pfam; PF13094; CENP-Q; 1.
PE 1: Evidence at protein level;
KW Centromere; Chromosome; Coiled coil; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..268
FT /note="Centromere protein Q"
FT /id="PRO_0000089534"
FT REGION 1..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 170..206
FT /evidence="ECO:0000255"
FT COMPBIAS 18..59
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 31
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231"
FT MOD_RES 50
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:25395579,
FT ECO:0007744|PubMed:21406692"
FT MOD_RES 249
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231,
FT ECO:0007744|PubMed:23186163"
FT VARIANT 63
FT /note="G -> R (in dbSNP:rs4267943)"
FT /id="VAR_022857"
FT VARIANT 266
FT /note="D -> G (in dbSNP:rs2501968)"
FT /id="VAR_024303"
FT MUTAGEN 50
FT /note="S->A: Abolishes the recruitment CENPE to
FT kinetochores but has no effect on recruitment of PLK1 to
FT knetochores."
FT /evidence="ECO:0000269|PubMed:25395579"
FT MUTAGEN 50
FT /note="S->D: No loss of the recruitment CENPE to
FT kinetochores."
FT /evidence="ECO:0000269|PubMed:25395579"
SQ SEQUENCE 268 AA; 30595 MW; 5B3735279287B3E8 CRC64;
MSGKANASKK NAQQLKRNPK RKKDNEEVVL SENKVRNTVK KNKNHLKDLS SEGQTKHTNL
KHGKTAASKR KTWQPLSKST RDHLQTMMES VIMTILSNSI KEKEEIQYHL NFLKKRLLQQ
CETLKVPPKK MEDLTNVSSL LNMERARDKA NEEGLALLQE EIDKMVETTE LMTGNIQSLK
NKIQILASEV EEEEERVKQM HQINSSGVLS LPELSQKTLK APTLQKEILA LIPNQNALLK
DLDILHNSSQ MKSMSTFIEE AYKKLDAS
