CENPS_ARATH
ID CENPS_ARATH Reviewed; 242 AA.
AC Q9FI55;
DT 10-MAY-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Protein MHF1 homolog {ECO:0000303|PubMed:24635147};
DE Short=AtMHF1 {ECO:0000303|PubMed:24635147};
GN Name=MHF1 {ECO:0000303|PubMed:24635147};
GN OrderedLocusNames=At5g50930 {ECO:0000312|Araport:AT5G50930};
GN ORFNames=K3K7.8 {ECO:0000312|EMBL:AED96012.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24635147; DOI=10.1111/tpj.12507;
RA Dangel N.J., Knoll A., Puchta H.;
RT "MHF1 plays Fanconi anaemia complementation group M protein (FANCM)-
RT dependent and FANCM-independent roles in DNA repair and homologous
RT recombination in plants.";
RL Plant J. 78:822-833(2014).
RN [4]
RP FUNCTION.
RX PubMed=25038251; DOI=10.1093/nar/gku614;
RA Girard C., Crismani W., Froger N., Mazel J., Lemhemdi A., Horlow C.,
RA Mercier R.;
RT "FANCM-associated proteins MHF1 and MHF2, but not the other Fanconi anemia
RT factors, limit meiotic crossovers.";
RL Nucleic Acids Res. 42:9087-9095(2014).
CC -!- FUNCTION: Involved in the promotion of spontaneous somatic homologous
CC recombination (HR) events, which is opposite to the function of FANCM
CC in ordered HR. Only FANCM is essential for replicative repair in the
CC absence of the endonuclease MUS81 (PubMed:24635147). Acts in the same
CC pathway as FANCM to restrain class II meiotic crossing over (CO), and
CC acts with FANCM during meiosis to repair interstrand cross-links (ICLs)
CC (PubMed:24635147, PubMed:25038251). This common pathway between MHF1
CC and FANCM is in parallel to the pathway that involves the RECQ4A
CC helicase (PubMed:24635147). {ECO:0000269|PubMed:24635147,
CC ECO:0000269|PubMed:25038251}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:24635147}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but mutant plants exhibit a defect in somatic homologous
CC recombination. {ECO:0000269|PubMed:24635147}.
CC -!- SIMILARITY: Belongs to the TAF9 family. CENP-S/MHF1 subfamily.
CC {ECO:0000305}.
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DR EMBL; AB017063; BAB08739.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96012.1; -; Genomic_DNA.
DR RefSeq; NP_199906.1; NM_124472.4.
DR AlphaFoldDB; Q9FI55; -.
DR SMR; Q9FI55; -.
DR STRING; 3702.AT5G50930.1; -.
DR PaxDb; Q9FI55; -.
DR PRIDE; Q9FI55; -.
DR ProteomicsDB; 224480; -.
DR EnsemblPlants; AT5G50930.1; AT5G50930.1; AT5G50930.
DR GeneID; 835166; -.
DR Gramene; AT5G50930.1; AT5G50930.1; AT5G50930.
DR KEGG; ath:AT5G50930; -.
DR Araport; AT5G50930; -.
DR TAIR; locus:2157393; AT5G50930.
DR eggNOG; ENOG502S2ZK; Eukaryota.
DR HOGENOM; CLU_100369_1_0_1; -.
DR InParanoid; Q9FI55; -.
DR OMA; CFAMDVG; -.
DR OrthoDB; 1483465at2759; -.
DR PhylomeDB; Q9FI55; -.
DR PRO; PR:Q9FI55; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FI55; baseline and differential.
DR GO; GO:0071821; C:FANCM-MHF complex; IBA:GO_Central.
DR GO; GO:0043240; C:Fanconi anaemia nuclear complex; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0007129; P:homologous chromosome pairing at meiosis; IGI:TAIR.
DR GO; GO:0036297; P:interstrand cross-link repair; IMP:TAIR.
DR GO; GO:0006312; P:mitotic recombination; IMP:TAIR.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:TAIR.
DR GO; GO:0031297; P:replication fork processing; IBA:GO_Central.
DR GO; GO:0000712; P:resolution of meiotic recombination intermediates; IBA:GO_Central.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR029003; CENP-S/Mhf1.
DR InterPro; IPR009072; Histone-fold.
DR Pfam; PF15630; CENP-S; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA recombination; DNA repair; DNA-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..242
FT /note="Protein MHF1 homolog"
FT /id="PRO_0000440038"
FT REGION 208..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 209..232
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 242 AA; 27292 MW; 2AC65E96AEBC83BD CRC64;
MFNISYAKRN AKYLFKLLAW CMMIEEAGPN IGNKQKVVLQ LSKMLIMYII LHINAAFWTW
RGYLKRCVLH FVFPRIFLPL LSDSPTITQA KKPSYCFAMD VGGEDISDLQ VDQIVEEYSM
DDLIRDRFRL SAISIAEAEA KKNGMEIGGP VVACVADLAF KYAENVAKDL ELFAHHAGRK
VVNMDDVVLS AHRNDNLAAS LRSLCNELKA KEPQSERKRK KGSAKKEDKA SSSNAVRITT
DL
