CENPS_SCHPO
ID CENPS_SCHPO Reviewed; 110 AA.
AC O74807;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 2.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Inner kinetochore subunit mhf1;
DE AltName: Full=CENP-S homolog;
DE AltName: Full=Constitutive centromere-associated network protein mhf1;
DE AltName: Full=MHF histone-fold complex subunit 1 {ECO:0000305|PubMed:20347428};
GN Name=mhf1 {ECO:0000303|PubMed:20347428};
GN ORFNames=SPBC2D10.16 {ECO:0000312|PomBase:SPBC2D10.16};
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION.
RX PubMed=20347428; DOI=10.1016/j.molcel.2010.01.039;
RA Yan Z., Delannoy M., Ling C., Daee D., Osman F., Muniandy P.A., Shen X.,
RA Oostra A.B., Du H., Steltenpool J., Lin T., Schuster B., Decaillet C.,
RA Stasiak A., Stasiak A.Z., Stone S., Hoatlin M.E., Schindler D.,
RA Woodcock C.L., Joenje H., Sen R., de Winter J.P., Li L., Seidman M.M.,
RA Whitby M.C., Myung K., Constantinousend A., Wang W.;
RT "A histone-fold complex and FANCM form a conserved DNA-remodeling complex
RT to maintain genome stability.";
RL Mol. Cell 37:865-878(2010).
RN [3]
RP FUNCTION.
RX PubMed=22723423; DOI=10.1126/science.1220111;
RA Lorenz A., Osman F., Sun W., Nandi S., Steinacher R., Whitby M.C.;
RT "The fission yeast FANCM ortholog directs non-crossover recombination
RT during meiosis.";
RL Science 336:1585-1588(2012).
RN [4]
RP RETRACTED PAPER.
RX PubMed=24026537; DOI=10.1098/rsob.130102;
RA Bhattacharjee S., Osman F., Feeney L., Lorenz A., Bryer C., Whitby M.C.;
RT "MHF1-2/CENP-S-X performs distinct roles in centromere metabolism and
RT genetic recombination.";
RL Open Biol. 3:130102-130102(2013).
RN [5]
RP RETRACTION NOTICE OF PUBMED:24026537.
RX PubMed=29445032; DOI=10.1098/rsob.180010;
RA Bhattacharjee S., Osman F., Feeney L., Lorenz A., Bryer C., Whitby M.C.;
RL Open Biol. 8:180010-180010(2018).
CC -!- FUNCTION: Component of a FANCM-MHF complex that promotes gene
CC conversion at blocked replication forks, probably by reversal of the
CC stalled fork (Probable). FANCM-MHF promotes non-crossover recombination
CC (PubMed:22723423). {ECO:0000269|PubMed:22723423,
CC ECO:0000305|PubMed:20347428}.
CC -!- SUBUNIT: The MHF histone-fold complex is a heterotetramer of 2 mhf1-
CC mhf2 heterodimers (By similarity). Component of the inner kinetochore
CC constitutive centromere-associated network (CCAN) (also known as
CC central kinetochore Sim4 complex in fission yeast), which is composed
CC of at least cnl2, cnp3, cnp20, fta1, fta2, fta3, fta4, fta6, fta7,
CC mal2, mhf1, mhf2, mis6, mis15, mis17, sim4 and wip1 (By similarity).
CC {ECO:0000250|UniProtKB:Q3E835}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:22723423}.
CC -!- SIMILARITY: Belongs to the TAF9 family. CENP-S/MHF1 subfamily.
CC {ECO:0000305}.
CC -!- CAUTION: An article showing that FANCM-MHF promotes gene conversion at
CC blocked replication forks by fork reversal was withdrawn due to
CC genotype mislabeling of strains. Following strain regeneration,
CC although many phenotypes were not reproducible, the central
CC observations of the publication were confirmed.
CC {ECO:0000305|PubMed:24026537, ECO:0000305|PubMed:29445032}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CU329671; CAA21174.2; -; Genomic_DNA.
DR PIR; T40119; T40119.
DR RefSeq; NP_596235.2; NM_001022155.3.
DR AlphaFoldDB; O74807; -.
DR SMR; O74807; -.
DR BioGRID; 276971; 5.
DR STRING; 4896.SPBC2D10.16.1; -.
DR MaxQB; O74807; -.
DR PaxDb; O74807; -.
DR EnsemblFungi; SPBC2D10.16.1; SPBC2D10.16.1:pep; SPBC2D10.16.
DR GeneID; 2540443; -.
DR KEGG; spo:SPBC2D10.16; -.
DR PomBase; SPBC2D10.16; mhf1.
DR VEuPathDB; FungiDB:SPBC2D10.16; -.
DR eggNOG; ENOG502RS0B; Eukaryota.
DR HOGENOM; CLU_100369_3_0_1; -.
DR InParanoid; O74807; -.
DR OMA; MVWAQIE; -.
DR PhylomeDB; O74807; -.
DR PRO; PR:O74807; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0043505; C:CENP-A containing nucleosome; TAS:PomBase.
DR GO; GO:0071821; C:FANCM-MHF complex; IPI:PomBase.
DR GO; GO:0043240; C:Fanconi anaemia nuclear complex; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0045003; P:double-strand break repair via synthesis-dependent strand annealing; IMP:PomBase.
DR GO; GO:0031297; P:replication fork processing; ISS:PomBase.
DR GO; GO:0000712; P:resolution of meiotic recombination intermediates; ISS:PomBase.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR029003; CENP-S/Mhf1.
DR InterPro; IPR009072; Histone-fold.
DR Pfam; PF15630; CENP-S; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA recombination; DNA repair; DNA-binding; Meiosis; Nucleus;
KW Reference proteome.
FT CHAIN 1..110
FT /note="Inner kinetochore subunit mhf1"
FT /id="PRO_0000304030"
SQ SEQUENCE 110 AA; 12821 MW; 1F692321469B0CF4 CRC64;
MMEEERFKAE IFHVTQEVCN RTASELTESE SRNVIVDELF CVGVTEMVWE QIRVLAKDIE
AFAEHAGRKT VQPQDVLLCC RRNEGLYEII NNFHKESIKS KKKKKENSTT
