CENPT_SCHPO
ID CENPT_SCHPO Reviewed; 479 AA.
AC Q9HGK9; Q9UU65;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Inner kinetochore subunit cnp20;
DE AltName: Full=CENP-T homolog;
DE AltName: Full=Centromere protein 20;
DE AltName: Full=Constitutive centromere-associated network protein cnp20;
GN Name=cnp20; ORFNames=SPBC800.13;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 52-406, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=ATCC 38364 / 968;
RX PubMed=10759889; DOI=10.1046/j.1365-2443.2000.00317.x;
RA Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T.,
RA Hiraoka Y.;
RT "Large-scale screening of intracellular protein localization in living
RT fission yeast cells by the use of a GFP-fusion genomic DNA library.";
RL Genes Cells 5:169-190(2000).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Component of the kinetochore, a multiprotein complex that
CC assembles on centromeric DNA and attaches chromosomes to spindle
CC microtubules, mediating chromosome segregation and sister chromatid
CC segregation during meiosis and mitosis. Component of the inner
CC kinetochore constitutive centromere-associated network (CCAN), which
CC serves as a structural platform for outer kinetochore assembly.
CC {ECO:0000250|UniProtKB:P43618}.
CC -!- SUBUNIT: Component of the inner kinetochore constitutive centromere-
CC associated network (CCAN) (also known as central kinetochore Sim4
CC complex in fission yeast), which is composed of at least cnl2, cnp3,
CC cnp20, fta1, fta2, fta3, fta4, fta6, fta7, mal2, mhf1, mhf2, mis6,
CC mis15, mis17, sim4 and wip1. {ECO:0000250|UniProtKB:P43618}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome.
CC -!- SIMILARITY: Belongs to the CENP-T/CNN1 family. {ECO:0000305}.
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DR EMBL; CU329671; CAC01528.1; -; Genomic_DNA.
DR EMBL; AB027786; BAA87090.1; -; Genomic_DNA.
DR RefSeq; NP_595115.1; NM_001021022.2.
DR AlphaFoldDB; Q9HGK9; -.
DR SMR; Q9HGK9; -.
DR BioGRID; 277431; 14.
DR STRING; 4896.SPBC800.13.1; -.
DR iPTMnet; Q9HGK9; -.
DR MaxQB; Q9HGK9; -.
DR PaxDb; Q9HGK9; -.
DR PRIDE; Q9HGK9; -.
DR EnsemblFungi; SPBC800.13.1; SPBC800.13.1:pep; SPBC800.13.
DR GeneID; 2540915; -.
DR KEGG; spo:SPBC800.13; -.
DR PomBase; SPBC800.13; cnp20.
DR VEuPathDB; FungiDB:SPBC800.13; -.
DR eggNOG; ENOG502S8G5; Eukaryota.
DR HOGENOM; CLU_544181_0_0_1; -.
DR InParanoid; Q9HGK9; -.
DR OMA; EMQGNTS; -.
DR PRO; PR:Q9HGK9; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0043505; C:CENP-A containing nucleosome; TAS:PomBase.
DR GO; GO:0000775; C:chromosome, centromeric region; IDA:PomBase.
DR GO; GO:0000779; C:condensed chromosome, centromeric region; IDA:PomBase.
DR GO; GO:0000776; C:kinetochore; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0007059; P:chromosome segregation; IBA:GO_Central.
DR GO; GO:0051382; P:kinetochore assembly; IEA:InterPro.
DR GO; GO:0000278; P:mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; NAS:PomBase.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR028255; CENP-T.
DR InterPro; IPR035425; CENP-T/H4_C.
DR InterPro; IPR009072; Histone-fold.
DR PANTHER; PTHR46904; PTHR46904; 2.
DR Pfam; PF15511; CENP-T_C; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
PE 3: Inferred from homology;
KW Chromosome; Nucleus; Reference proteome.
FT CHAIN 1..479
FT /note="Inner kinetochore subunit cnp20"
FT /id="PRO_0000310364"
FT REGION 24..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 82..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 250..277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..42
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..57
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 128..143
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 263..277
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 479 AA; 54324 MW; D87277F7085225EB CRC64;
MASHNYETPL KSSSFQHLVG TLSQRRHFTP SRSRYTPRSA QRTVTPHKRR ALARRNSLAR
RRSNVFSATT PRDILRMLSR ALAKNPVPSP AESESSERRH TPQTNSQKSQ KTPRLSSNKR
RTLKNDAKQR NSQSPGTDTS DANLTIQSIE APRRAPQRLS DFFTPDGRRV SNIRDSLISE
KRLENNIDQV QESALSTASS NRLIDLKNDE VPIFRIPLSD YETDDMDDGT PLQRHVATLN
QYQSPIFNLG PDILEDEPSY SSRASRSRQS SLSSRLSELP SKRASLEILR RENTFPADPI
QEFGEKAYNE RELMEEISNF EPLLDDNLLE NANEAVNSPV VDAPMDADSA LEIPNDEDNG
EILNKLKDSP FKKPKRRYSK SSTLVLPETN IRKLANSYSQ KKIAGSVIEE LTTASELFFK
QIANDLSAFA DHAHRKTIDT QDVVLLMKRQ RKISEKKSLS SLMQQYLSRE IAPPAIKRT
