ID   CENPU_KLULA             Reviewed;         292 AA.
AC   Q6CUE5;
DT   31-JAN-2018, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Inner kinetochore subunit AME1;
DE   AltName: Full=CENP-U homolog;
DE   AltName: Full=Constitutive centromere-associated network protein AME1;
GN   Name=AME1; OrderedLocusNames=KLLA0C05500g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
RN   [2]
RP   SUBUNIT, INTERACTION WITH OKP1; NKP1 AND NKP2, AND FUNCTION.
RX   PubMed=29046335; DOI=10.15252/embj.201796636;
RA   Schmitzberger F., Richter M.M., Gordiyenko Y., Robinson C.V., Dadlez M.,
RA   Westermann S.;
RT   "Molecular basis for inner kinetochore configuration through RWD domain-
RT   peptide interactions.";
RL   EMBO J. 36:3458-3482(2017).
CC   -!- FUNCTION: Component of the kinetochore, a multiprotein complex that
CC       assembles on centromeric DNA and attaches chromosomes to spindle
CC       microtubules, mediating chromosome segregation and sister chromatid
CC       segregation during meiosis and mitosis (By similarity). Component of
CC       the inner kinetochore COMA subcomplex, which connects centromere-
CC       associated proteins and the outer kinetochore (PubMed:29046335). COMA
CC       interacts with other inner kinetochore proteins to form the inner
CC       kinetochore constitutive centromere-associated network (CCAN), which
CC       serves as a structural platform for outer kinetochore assembly (By
CC       similarity). {ECO:0000250|UniProtKB:P38313,
CC       ECO:0000269|PubMed:29046335}.
CC   -!- SUBUNIT: Component of the heterotetrameric kinetochore subcomplex COMA,
CC       which consists of AME1, CTF19, MCM21 and OKP1 (PubMed:29046335). The
CC       COMA subcomplex is part of a larger constitutive centromere-associated
CC       network (CCAN) (also known as central kinetochore CTF19 complex in
CC       yeast) (By similarity). COMA binds the centromeric nucleosome-binding
CC       protein MIF2, and to the outer kinetochore MIND subcomplex, probably
CC       via AME1. AME1 interacts directly with OKP1 and an NKP1-NKP2 dimer
CC       (PubMed:29046335). {ECO:0000250|UniProtKB:P38313,
CC       ECO:0000269|PubMed:29046335}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P38313}.
CC       Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:P38313}.
CC   -!- SIMILARITY: Belongs to the CENP-U/AME1 family. {ECO:0000305}.
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DR   EMBL; CR382123; CAH01295.1; -; Genomic_DNA.
DR   RefSeq; XP_452444.1; XM_452444.1.
DR   AlphaFoldDB; Q6CUE5; -.
DR   SMR; Q6CUE5; -.
DR   IntAct; Q6CUE5; 1.
DR   MINT; Q6CUE5; -.
DR   EnsemblFungi; CAH01295; CAH01295; KLLA0_C05500g.
DR   GeneID; 2892623; -.
DR   KEGG; kla:KLLA0_C05500g; -.
DR   HOGENOM; CLU_953353_0_0_1; -.
DR   InParanoid; Q6CUE5; -.
DR   OMA; HFVDLMD; -.
DR   Proteomes; UP000000598; Chromosome C.
DR   GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Kinetochore; Meiosis;
KW   Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..292
FT                   /note="Inner kinetochore subunit AME1"
FT                   /id="PRO_0000443077"
FT   REGION          42..63
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          129..247
FT                   /note="Interaction with OKP1"
FT                   /evidence="ECO:0000269|PubMed:29046335"
FT   REGION          268..292
FT                   /note="Interaction with NKP1-NKP2"
FT                   /evidence="ECO:0000269|PubMed:29046335"
SQ   SEQUENCE   292 AA;  32853 MW;  69C5495FFA97D392 CRC64;
     MDALKQRHLK LLYRQRGSAS RTIDYDVVIE PELREDGDLV TDSQLTEQDD QSFPDIQVPS
     PDRVDDFDLN QGDGLMDKAT ANEDDIDHVI GDEVAAVNAP VETSDLVIDT TQFDSMTLSQ
     LSTPLTSMAT IDVLRRLLET VILVTIEERT SQFNRAGSAR LRLKLKLEIK ILTWFLHQSK
     EDLITISELN LSNNDLLYRL KQISLLKNSL SRSLLDVRSA ISSYEGPNNA LSEEQVALQN
     FKRINELILS QRIDTIINNN LPPDTSTFDT ESKKGLLPTL KTLNESLQNT IG