CENPW_SCHPO
ID CENPW_SCHPO Reviewed; 73 AA.
AC G2TRL2;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2011, sequence version 1.
DT 25-MAY-2022, entry version 42.
DE RecName: Full=Inner kinetochore subunit wip1;
DE AltName: Full=CENP-W homolog;
DE AltName: Full=Constitutive centromere-associated network protein wip1;
DE AltName: Full=Histone-fold domain-containing protein new1;
GN Name=wip1; Synonyms=new1; ORFNames=SPAC17G8.15;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP IDENTIFICATION.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
RN [3]
RP IDENTIFICATION, AND DISRUPTION PHENOTYPE.
RX PubMed=21270388; DOI=10.1534/genetics.110.123497;
RA Bitton D.A., Wood V., Scutt P.J., Grallert A., Yates T., Smith D.L.,
RA Hagan I.M., Miller C.J.;
RT "Augmented annotation of the Schizosaccharomyces pombe genome reveals
RT additional genes required for growth and viability.";
RL Genetics 187:1207-1217(2011).
CC -!- FUNCTION: Component of the kinetochore, a multiprotein complex that
CC assembles on centromeric DNA and attaches chromosomes to spindle
CC microtubules, mediating chromosome segregation and sister chromatid
CC segregation during meiosis and mitosis. Component of the inner
CC kinetochore constitutive centromere-associated network (CCAN), which
CC serves as a structural platform for outer kinetochore assembly.
CC {ECO:0000250|UniProtKB:Q2V2P8}.
CC -!- SUBUNIT: Component of the inner kinetochore constitutive centromere-
CC associated network (CCAN) (also known as central kinetochore Sim4
CC complex in fission yeast), which is composed of at least cnl2, cnp3,
CC cnp20, fta1, fta2, fta3, fta4, fta6, fta7, mal2, mhf1, mhf2, mis6,
CC mis15, mis17, sim4 and wip1. {ECO:0000250|UniProtKB:Q2V2P8}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q2V2P8}.
CC Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q2V2P8}.
CC -!- DISRUPTION PHENOTYPE: Severely perturbed cell division. Aberrant cell-
CC wall deposits accumulate during septation alongside a range of mitotic
CC defects including errors in chromosome segregation and spindle
CC formation. Slow growth at 25 degrees and inviable at 32 degrees.
CC {ECO:0000269|PubMed:21270388}.
CC -!- SIMILARITY: Belongs to the CENP-W/WIP1 family. {ECO:0000305}.
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DR EMBL; CU329670; CCD31317.1; -; Genomic_DNA.
DR RefSeq; XP_004001772.1; XM_004001723.1.
DR AlphaFoldDB; G2TRL2; -.
DR SMR; G2TRL2; -.
DR STRING; 4896.SPAC17G8.15.1; -.
DR PaxDb; G2TRL2; -.
DR EnsemblFungi; SPAC17G8.15.1; SPAC17G8.15.1:pep; SPAC17G8.15.
DR PomBase; SPAC17G8.15; wip1.
DR VEuPathDB; FungiDB:SPAC17G8.15; -.
DR eggNOG; ENOG502SEGJ; Eukaryota.
DR HOGENOM; CLU_159428_1_1_1; -.
DR InParanoid; G2TRL2; -.
DR OMA; YILFMQE; -.
DR PRO; PR:G2TRL2; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0043505; C:CENP-A containing nucleosome; TAS:PomBase.
DR GO; GO:0000776; C:kinetochore; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IC:PomBase.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0007059; P:chromosome segregation; IBA:GO_Central.
DR GO; GO:0051382; P:kinetochore assembly; IBA:GO_Central.
DR GO; GO:0000278; P:mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; ISO:PomBase.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR003958; CBFA_NFYB_domain.
DR InterPro; IPR009072; Histone-fold.
DR Pfam; PF00808; CBFD_NFYB_HMF; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
PE 2: Evidence at transcript level;
KW Centromere; Chromosome; Kinetochore; Nucleus; Reference proteome.
FT CHAIN 1..73
FT /note="Inner kinetochore subunit wip1"
FT /id="PRO_0000416502"
FT DOMAIN 1..67
FT /note="Histone-fold"
SQ SEQUENCE 73 AA; 8640 MW; F1575F82AD73B2B6 CRC64;
MSYPKARIRR FFRQHCQRSL ESSALDLVYL DYCLFLQSLL KEANIEAAKT GERRVQPEHV
RSIQRKILAQ FKG
