CENPW_YEAST
ID CENPW_YEAST Reviewed; 89 AA.
AC Q2V2P8; D6VT06;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2006, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Inner kinetochore subunit WIP1 {ECO:0000305};
DE AltName: Full=CENP-W homolog {ECO:0000303|PubMed:22561346};
DE AltName: Full=Constitutive centromere-associated network protein WIP1 {ECO:0000305};
DE AltName: Full=W-like protein 1 {ECO:0000303|PubMed:22561346};
GN Name=WIP1 {ECO:0000303|PubMed:22561346};
GN OrderedLocusNames=YDR374W-A {ECO:0000312|SGD:S000113553};
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP GENOME REANNOTATION.
RX PubMed=12775844; DOI=10.1126/science.1084337;
RA Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J.,
RA Waterston R., Cohen B.A., Johnston M.;
RT "Finding functional features in Saccharomyces genomes by phylogenetic
RT footprinting.";
RL Science 301:71-76(2003).
RN [4]
RP IDENTIFICATION IN CCAN, AND SUBUNIT.
RX PubMed=22561346; DOI=10.1038/ncb2493;
RA Schleiffer A., Maier M., Litos G., Lampert F., Hornung P., Mechtler K.,
RA Westermann S.;
RT "CENP-T proteins are conserved centromere receptors of the Ndc80 complex.";
RL Nat. Cell Biol. 14:604-613(2012).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Component of the kinetochore, a multiprotein complex that
CC assembles on centromeric DNA and attaches chromosomes to spindle
CC microtubules, mediating chromosome segregation and sister chromatid
CC segregation during meiosis and mitosis. Component of the inner
CC kinetochore constitutive centromere-associated network (CCAN), which
CC serves as a structural platform for outer kinetochore assembly
CC (PubMed:22561346). {ECO:0000269|PubMed:22561346}.
CC -!- SUBUNIT: Component of the inner kinetochore constitutive centromere-
CC associated network (CCAN) (also known as central kinetochore CTF19
CC complex in yeast), which is composed of at least AME1, CHL4, CNN1,
CC CTF3, CTF19, IML3, MCM16, MCM21, MCM22, MHF1, MHF2, MIF2, NKP1, NKP2,
CC OKP1 and WIP1. {ECO:0000269|PubMed:22561346}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:22561346}.
CC Chromosome, centromere, kinetochore {ECO:0000305|PubMed:22561346}.
CC -!- SIMILARITY: Belongs to the CENP-W/WIP1 family. {ECO:0000305}.
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DR EMBL; U28373; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BK006938; DAA12216.1; -; Genomic_DNA.
DR RefSeq; NP_001032576.1; NM_001184680.1.
DR PDB; 6WUC; EM; 3.23 A; W=1-89.
DR PDB; 6YPC; X-ray; 2.90 A; W=1-89.
DR PDBsum; 6WUC; -.
DR PDBsum; 6YPC; -.
DR AlphaFoldDB; Q2V2P8; -.
DR SMR; Q2V2P8; -.
DR BioGRID; 531942; 7.
DR STRING; 4932.YDR374W-A; -.
DR iPTMnet; Q2V2P8; -.
DR MaxQB; Q2V2P8; -.
DR PaxDb; Q2V2P8; -.
DR PRIDE; Q2V2P8; -.
DR EnsemblFungi; YDR374W-A_mRNA; YDR374W-A; YDR374W-A.
DR GeneID; 3799971; -.
DR KEGG; sce:YDR374W-A; -.
DR SGD; S000113553; WIP1.
DR VEuPathDB; FungiDB:YDR374W-A; -.
DR HOGENOM; CLU_189349_0_0_1; -.
DR InParanoid; Q2V2P8; -.
DR BioCyc; YEAST:G3O-30126-MON; -.
DR ChiTaRS; WIP1; yeast.
DR PRO; PR:Q2V2P8; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q2V2P8; protein.
DR GO; GO:0000776; C:kinetochore; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0019237; F:centromeric DNA binding; IDA:SGD.
DR GO; GO:0007059; P:chromosome segregation; IGI:SGD.
PE 1: Evidence at protein level;
KW 3D-structure; Centromere; Chromosome; Kinetochore; Nucleus;
KW Reference proteome.
FT CHAIN 1..89
FT /note="Inner kinetochore subunit WIP1"
FT /id="PRO_0000253848"
FT HELIX 3..14
FT /evidence="ECO:0007829|PDB:6YPC"
FT HELIX 16..21
FT /evidence="ECO:0007829|PDB:6WUC"
FT HELIX 37..67
FT /evidence="ECO:0007829|PDB:6YPC"
FT HELIX 75..88
FT /evidence="ECO:0007829|PDB:6YPC"
SQ SEQUENCE 89 AA; 10244 MW; F2F4911A26576318 CRC64;
MDTEALANYL LRQLSLDAEE NKLEDLLQRQ NEDQESSQEY NKKLLLACGF QAILRKILLD
ARTRATAEGL REVYPYHIEA ATQAFLDSQ
