CENPX_SCHPO
ID CENPX_SCHPO Reviewed; 89 AA.
AC O74896;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Inner kinetochore subunit mhf2;
DE AltName: Full=CENP-X homolog;
DE AltName: Full=Constitutive centromere-associated network protein mhf2;
DE AltName: Full=MHF histone-fold complex subunit 2 {ECO:0000305|PubMed:20347428};
GN Name=mhf2 {ECO:0000303|PubMed:20347428};
GN ORFNames=SPCC576.12c {ECO:0000312|PomBase:SPCC576.12c};
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [3]
RP FUNCTION.
RX PubMed=20347428; DOI=10.1016/j.molcel.2010.01.039;
RA Yan Z., Delannoy M., Ling C., Daee D., Osman F., Muniandy P.A., Shen X.,
RA Oostra A.B., Du H., Steltenpool J., Lin T., Schuster B., Decaillet C.,
RA Stasiak A., Stasiak A.Z., Stone S., Hoatlin M.E., Schindler D.,
RA Woodcock C.L., Joenje H., Sen R., de Winter J.P., Li L., Seidman M.M.,
RA Whitby M.C., Myung K., Constantinousend A., Wang W.;
RT "A histone-fold complex and FANCM form a conserved DNA-remodeling complex
RT to maintain genome stability.";
RL Mol. Cell 37:865-878(2010).
RN [4]
RP FUNCTION.
RX PubMed=22723423; DOI=10.1126/science.1220111;
RA Lorenz A., Osman F., Sun W., Nandi S., Steinacher R., Whitby M.C.;
RT "The fission yeast FANCM ortholog directs non-crossover recombination
RT during meiosis.";
RL Science 336:1585-1588(2012).
RN [5]
RP RETRACTED PAPER.
RX PubMed=24026537; DOI=10.1098/rsob.130102;
RA Bhattacharjee S., Osman F., Feeney L., Lorenz A., Bryer C., Whitby M.C.;
RT "MHF1-2/CENP-S-X performs distinct roles in centromere metabolism and
RT genetic recombination.";
RL Open Biol. 3:130102-130102(2013).
RN [6]
RP RETRACTION NOTICE OF PUBMED:24026537.
RX PubMed=29445032; DOI=10.1098/rsob.180010;
RA Bhattacharjee S., Osman F., Feeney L., Lorenz A., Bryer C., Whitby M.C.;
RL Open Biol. 8:180010-180010(2018).
CC -!- FUNCTION: Component of a FANCM-MHF complex that promotes gene
CC conversion at blocked replication forks, probably by reversal of the
CC stalled fork (Probable). FANCM-MHF promotes non-crossover recombination
CC (PubMed:22723423). {ECO:0000269|PubMed:22723423,
CC ECO:0000305|PubMed:20347428}.
CC -!- SUBUNIT: The MHF histone-fold complex is a heterotetramer of 2 mhf1-
CC mhf2 heterodimers (By similarity). Component of the inner kinetochore
CC constitutive centromere-associated network (CCAN) (also known as
CC central kinetochore Sim4 complex in fission yeast), which is composed
CC of at least cnl2, cnp3, cnp20, fta1, fta2, fta3, fta4, fta6, fta7,
CC mal2, mhf1, mhf2, mis6, mis15, mis17, sim4 and wip1 (By similarity).
CC {ECO:0000250|UniProtKB:Q3E835}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16823372}. Cytoplasm
CC {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the CENP-X/MHF2 family. {ECO:0000305}.
CC -!- CAUTION: An article showing that FANCM-MHF promotes gene conversion at
CC blocked replication forks by fork reversal was withdrawn due to
CC genotype mislabeling of strains. Following strain regeneration,
CC although many phenotypes were not reproducible, the central
CC observations of the publication were confirmed.
CC {ECO:0000305|PubMed:24026537, ECO:0000305|PubMed:29445032}.
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DR EMBL; CU329672; CAA21191.1; -; Genomic_DNA.
DR PIR; T41422; T41422.
DR RefSeq; NP_588439.1; NM_001023430.2.
DR AlphaFoldDB; O74896; -.
DR SMR; O74896; -.
DR BioGRID; 275973; 61.
DR STRING; 4896.SPCC576.12c.1; -.
DR MaxQB; O74896; -.
DR PaxDb; O74896; -.
DR EnsemblFungi; SPCC576.12c.1; SPCC576.12c.1:pep; SPCC576.12c.
DR GeneID; 2539408; -.
DR KEGG; spo:SPCC576.12c; -.
DR PomBase; SPCC576.12c; mhf2.
DR VEuPathDB; FungiDB:SPCC576.12c; -.
DR eggNOG; ENOG502S98G; Eukaryota.
DR HOGENOM; CLU_2470378_0_0_1; -.
DR InParanoid; O74896; -.
DR OMA; TKEAVCR; -.
DR PhylomeDB; O74896; -.
DR PRO; PR:O74896; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0043505; C:CENP-A containing nucleosome; TAS:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0071821; C:FANCM-MHF complex; IPI:PomBase.
DR GO; GO:0043240; C:Fanconi anaemia nuclear complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0045003; P:double-strand break repair via synthesis-dependent strand annealing; IMP:PomBase.
DR GO; GO:0051382; P:kinetochore assembly; IEA:InterPro.
DR GO; GO:0031297; P:replication fork processing; IGI:PomBase.
DR GO; GO:0000712; P:resolution of meiotic recombination intermediates; IGI:PomBase.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR018552; CENP-X.
DR InterPro; IPR009072; Histone-fold.
DR Pfam; PF09415; CENP-X; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA damage; DNA recombination; DNA repair; DNA-binding; Meiosis;
KW Nucleus; Reference proteome.
FT CHAIN 1..89
FT /note="Inner kinetochore subunit mhf2"
FT /id="PRO_0000372347"
SQ SEQUENCE 89 AA; 10051 MW; B131E2163699D1BD CRC64;
MEQESELLSL NTLARILTLY FSSEKGTKIT PSALELITQY LRIYSKEAVC RAYEEKKNSI
MSSSENEDIV LELEDLENGI AAQLALDFS
