CEP63_DANRE
ID CEP63_DANRE Reviewed; 716 AA.
AC Q6PGZ0;
DT 01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-SEP-2009, sequence version 2.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Centrosomal protein of 63 kDa;
DE Short=Cep63;
GN Name=cep63; ORFNames=zgc:63548;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=AB;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-263 (ISOFORM 1).
RC STRAIN=Tuebingen; TISSUE=Embryo;
RA Mathavan S., Yao F., Wong E., Thoreau H., Nayudu M., Govindarajan K.R.,
RA Ruan Y., Wei C.;
RT "Genome Institute of Singapore, zebrafish transcriptome characterization.";
RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Required for normal spindle assembly. Plays a key role in
CC mother-centriole-dependent centriole duplication. Plays a role in DNA
CC damage response. Following DNA damage, such as double-strand breaks
CC (DSBs), is removed from centrosomes; this leads to the inactivation of
CC spindle assembly and delay in mitotic progression (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000250}. Cytoplasm, cytoskeleton, microtubule
CC organizing center, centrosome, centriole {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6PGZ0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6PGZ0-2; Sequence=VSP_037847;
CC -!- SIMILARITY: Belongs to the CEP63 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EH580184; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; BC056780; AAH56780.1; -; mRNA.
DR EMBL; EH580184; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_956896.1; NM_200602.1. [Q6PGZ0-2]
DR AlphaFoldDB; Q6PGZ0; -.
DR SMR; Q6PGZ0; -.
DR STRING; 7955.ENSDARP00000109882; -.
DR PaxDb; Q6PGZ0; -.
DR GeneID; 393574; -.
DR KEGG; dre:393574; -.
DR CTD; 80254; -.
DR ZFIN; ZDB-GENE-040426-1154; cep63.
DR eggNOG; ENOG502QRYU; Eukaryota.
DR InParanoid; Q6PGZ0; -.
DR OrthoDB; 943593at2759; -.
DR PhylomeDB; Q6PGZ0; -.
DR Reactome; R-DRE-2565942; Regulation of PLK1 Activity at G2/M Transition.
DR Reactome; R-DRE-380259; Loss of Nlp from mitotic centrosomes.
DR Reactome; R-DRE-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-DRE-380284; Loss of proteins required for interphase microtubule organization from the centrosome.
DR Reactome; R-DRE-380320; Recruitment of NuMA to mitotic centrosomes.
DR Reactome; R-DRE-5620912; Anchoring of the basal body to the plasma membrane.
DR Reactome; R-DRE-8854518; AURKA Activation by TPX2.
DR PRO; PR:Q6PGZ0; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0005814; C:centriole; ISS:UniProtKB.
DR GO; GO:0005813; C:centrosome; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000922; C:spindle pole; ISS:UniProtKB.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007099; P:centriole replication; ISS:UniProtKB.
DR GO; GO:0098535; P:de novo centriole assembly involved in multi-ciliated epithelial cell differentiation; IBA:GO_Central.
DR GO; GO:0000077; P:DNA damage checkpoint signaling; ISS:UniProtKB.
DR GO; GO:0042770; P:signal transduction in response to DNA damage; ISS:UniProtKB.
DR GO; GO:0051225; P:spindle assembly; ISS:UniProtKB.
DR InterPro; IPR029608; Cep63.
DR InterPro; IPR031470; Cep63/Deup1_N.
DR PANTHER; PTHR18875:SF7; PTHR18875:SF7; 1.
DR Pfam; PF17045; CEP63; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell cycle; Cell division; Coiled coil; Cytoplasm;
KW Cytoskeleton; DNA damage; Mitosis; Reference proteome.
FT CHAIN 1..716
FT /note="Centrosomal protein of 63 kDa"
FT /id="PRO_0000381808"
FT REGION 587..626
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 38..394
FT /evidence="ECO:0000255"
FT COILED 426..590
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..27
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_037847"
FT CONFLICT 35
FT /note="M -> V (in Ref. 2; EH580184)"
FT /evidence="ECO:0000305"
FT CONFLICT 39
FT /note="R -> Q (in Ref. 2; EH580184)"
FT /evidence="ECO:0000305"
FT CONFLICT 86
FT /note="T -> A (in Ref. 2; EH580184)"
FT /evidence="ECO:0000305"
FT CONFLICT 192
FT /note="Q -> H (in Ref. 2; EH580184)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 716 AA; 83092 MW; E8EF3918C84692D7 CRC64;
MEEFLSTLRD HDASSVLSSC EPELQELMRQ IDIMMGHKRH EWEAEVRAME LSLHNTQEEL
QSAKALLDKR NSEIRVLGKQ LEELHTGKQE LVVKYEEQLL HVKEELSKLK RSYEKLQRKH
LKESRDGALS REEDRTELSR LNSKIEEFRQ RSAEWEQQRL QYQRQVSLLE EQRKTLAEQF
SLIQSQGVGR PQEQGQGELQ RLRSQLQRAQ DSLHAQELEL ERLRLLQDEL GDSIKEQQVS
CHAAAHRRGQ VLSEEREELK ATLDAQDQFV RSTGVQQQQL RREVNRLNQT LQAKEQVIRS
LEECLSSPGS APNLASLRQD LEKVTARLNS SQTCESHLKA EVMRLRDKLE SMRKYKAEMS
RREHEWKQME EEHSRCTAEN KRLRDELERA EQTRCGEQEG MRKEVFQLTS ELHQRDITIA
TLTGSTSSIE RQLRAEVERA ERRASELKVT QVQLETLKLE NQHLNDLLER VESQSPKRGD
GELASLRDSY VSSLKSLEEE NRQLRLEMTE LRARMEASNQ TWQDKYERAL LQNQNKNHLY
NEQNRADEDV QRRHQEELQA METQMQERAS HYEEQIQTLL TQLENLSRTS PYRPDGQTQE
SKTSVSPARS SASSASSSSS TRKAQRVATL LSAVANGEEA QASSSDSTGS PNTSVTTRFL
EEETLRSQEL LQRLDAHIQS MKLENSNTVC KYLGKTGNPQ NDVFWQGTHS FCKMID
