CEP95_HUMAN
ID CEP95_HUMAN Reviewed; 821 AA.
AC Q96GE4; B4DMD2; Q96M81;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Centrosomal protein of 95 kDa;
DE Short=Cep95;
DE AltName: Full=Coiled-coil domain-containing protein 45;
GN Name=CEP95; Synonyms=CCDC45, CEP45;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1-607 (ISOFORM 1).
RC TISSUE=Brain, and Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16625196; DOI=10.1038/nature04689;
RA Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R.,
RA Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A.,
RA Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J.,
RA Chang J.L., Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J.,
RA DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S.,
RA Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E.,
RA Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K.,
RA LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J.,
RA Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A.,
RA Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K.,
RA Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D.,
RA Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A.,
RA Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.;
RT "DNA sequence of human chromosome 17 and analysis of rearrangement in the
RT human lineage.";
RL Nature 440:1045-1049(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Lymph;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Lymphoblast;
RX PubMed=14654843; DOI=10.1038/nature02166;
RA Andersen J.S., Wilkinson C.J., Mayor T., Mortensen P., Nigg E.A., Mann M.;
RT "Proteomic characterization of the human centrosome by protein correlation
RT profiling.";
RL Nature 426:570-574(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-449; SER-451 AND SER-453, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=21399614; DOI=10.1038/emboj.2011.63;
RA Jakobsen L., Vanselow K., Skogs M., Toyoda Y., Lundberg E., Poser I.,
RA Falkenby L.G., Bennetzen M., Westendorf J., Nigg E.A., Uhlen M.,
RA Hyman A.A., Andersen J.S.;
RT "Novel asymmetrically localizing components of human centrosomes identified
RT by complementary proteomics methods.";
RL EMBO J. 30:1520-1535(2011).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-449 AND SER-451, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
CC -!- INTERACTION:
CC Q96GE4; Q9BWT7: CARD10; NbExp=3; IntAct=EBI-372775, EBI-3866279;
CC Q96GE4; Q8NA61: CBY2; NbExp=3; IntAct=EBI-372775, EBI-741724;
CC Q96GE4; Q86Z20: CCDC125; NbExp=3; IntAct=EBI-372775, EBI-11977221;
CC Q96GE4; Q8IZU0: FAM9B; NbExp=4; IntAct=EBI-372775, EBI-10175124;
CC Q96GE4; Q08379: GOLGA2; NbExp=3; IntAct=EBI-372775, EBI-618309;
CC Q96GE4; A6NEM1: GOLGA6L9; NbExp=3; IntAct=EBI-372775, EBI-5916454;
CC Q96GE4; Q9NP66: HMG20A; NbExp=3; IntAct=EBI-372775, EBI-740641;
CC Q96GE4; Q96ED9-2: HOOK2; NbExp=3; IntAct=EBI-372775, EBI-10961706;
CC Q96GE4; Q7Z3Y8: KRT27; NbExp=3; IntAct=EBI-372775, EBI-3044087;
CC Q96GE4; P23508: MCC; NbExp=3; IntAct=EBI-372775, EBI-307531;
CC Q96GE4; Q5JR59: MTUS2; NbExp=3; IntAct=EBI-372775, EBI-742948;
CC Q96GE4; Q5JR59-3: MTUS2; NbExp=4; IntAct=EBI-372775, EBI-11522433;
CC Q96GE4; Q9NRD5: PICK1; NbExp=3; IntAct=EBI-372775, EBI-79165;
CC Q96GE4; Q9UBB9: TFIP11; NbExp=3; IntAct=EBI-372775, EBI-1105213;
CC Q96GE4; Q05BL1: TP53BP2; NbExp=3; IntAct=EBI-372775, EBI-11952721;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000269|PubMed:14654843,
CC ECO:0000269|PubMed:21399614}. Cytoplasm, cytoskeleton, spindle pole
CC {ECO:0000269|PubMed:21399614}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q96GE4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96GE4-2; Sequence=VSP_056356;
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DR EMBL; AK057326; BAB71428.1; -; mRNA.
DR EMBL; AK297409; BAG59844.1; -; mRNA.
DR EMBL; AC009994; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC009518; AAH09518.1; -; mRNA.
DR CCDS; CCDS45763.1; -. [Q96GE4-1]
DR RefSeq; NP_001303919.1; NM_001316990.1. [Q96GE4-2]
DR RefSeq; NP_612372.1; NM_138363.2. [Q96GE4-1]
DR AlphaFoldDB; Q96GE4; -.
DR SMR; Q96GE4; -.
DR BioGRID; 124762; 51.
DR IntAct; Q96GE4; 33.
DR STRING; 9606.ENSP00000450461; -.
DR iPTMnet; Q96GE4; -.
DR PhosphoSitePlus; Q96GE4; -.
DR BioMuta; CEP95; -.
DR DMDM; 74751882; -.
DR EPD; Q96GE4; -.
DR jPOST; Q96GE4; -.
DR MassIVE; Q96GE4; -.
DR MaxQB; Q96GE4; -.
DR PaxDb; Q96GE4; -.
DR PeptideAtlas; Q96GE4; -.
DR PRIDE; Q96GE4; -.
DR ProteomicsDB; 4597; -.
DR ProteomicsDB; 76624; -. [Q96GE4-1]
DR Antibodypedia; 67088; 88 antibodies from 20 providers.
DR DNASU; 90799; -.
DR Ensembl; ENST00000556440.7; ENSP00000450461.2; ENSG00000258890.8. [Q96GE4-1]
DR GeneID; 90799; -.
DR KEGG; hsa:90799; -.
DR MANE-Select; ENST00000556440.7; ENSP00000450461.2; NM_138363.3; NP_612372.1.
DR UCSC; uc002jem.4; human. [Q96GE4-1]
DR CTD; 90799; -.
DR DisGeNET; 90799; -.
DR GeneCards; CEP95; -.
DR HGNC; HGNC:25141; CEP95.
DR HPA; ENSG00000258890; Low tissue specificity.
DR neXtProt; NX_Q96GE4; -.
DR OpenTargets; ENSG00000258890; -.
DR PharmGKB; PA142672162; -.
DR VEuPathDB; HostDB:ENSG00000258890; -.
DR eggNOG; ENOG502QTFE; Eukaryota.
DR GeneTree; ENSGT00390000005412; -.
DR HOGENOM; CLU_018428_1_0_1; -.
DR InParanoid; Q96GE4; -.
DR OMA; KHSQPWK; -.
DR OrthoDB; 1353802at2759; -.
DR PhylomeDB; Q96GE4; -.
DR TreeFam; TF333224; -.
DR PathwayCommons; Q96GE4; -.
DR SignaLink; Q96GE4; -.
DR BioGRID-ORCS; 90799; 10 hits in 1075 CRISPR screens.
DR ChiTaRS; CEP95; human.
DR GenomeRNAi; 90799; -.
DR Pharos; Q96GE4; Tdark.
DR PRO; PR:Q96GE4; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q96GE4; protein.
DR Bgee; ENSG00000258890; Expressed in right uterine tube and 170 other tissues.
DR ExpressionAtlas; Q96GE4; baseline and differential.
DR Genevisible; Q96GE4; HS.
DR GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000922; C:spindle pole; IDA:UniProtKB.
DR InterPro; IPR026619; CEP95.
DR InterPro; IPR044039; DUF5745.
DR PANTHER; PTHR22545; PTHR22545; 1.
DR Pfam; PF19016; DUF5745; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..821
FT /note="Centrosomal protein of 95 kDa"
FT /id="PRO_0000234505"
FT REGION 118..149
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 323..369
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 395..475
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 585..632
FT /evidence="ECO:0000255"
FT COILED 700..794
FT /evidence="ECO:0000255"
FT COMPBIAS 343..359
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 449
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:21406692"
FT MOD_RES 451
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:21406692"
FT MOD_RES 453
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT VAR_SEQ 1..164
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056356"
FT VARIANT 165
FT /note="M -> I (in dbSNP:rs9910506)"
FT /id="VAR_033666"
FT VARIANT 811
FT /note="A -> V (in dbSNP:rs11550922)"
FT /id="VAR_050751"
SQ SEQUENCE 821 AA; 95297 MW; 789AB2EF7E057F51 CRC64;
MAGSDAEWVT IANNLLFKCH IHLRIHELQD CDANVFIALY QSILGEKVPD LIVIPRSQED
DAHNVQAVID SLALDYLQVS LSHITGENIV KGDKESIKNL LEIFDGLLEY LTERISETSH
EKSETEQYFK ESDRGERLEE PESTKESKSS WKRVSFGRCS LSSEMLGPSW DGDEAESTGE
IIRLGDTAHT FSLRSNGAQC PNEMLSKKAL ASPSSKSHED MLYPPSVLSK SRTSFVEDTE
TLSVSGIPNA RKLGEPIRAA IPLHPPYHPS EPRAPCPIGK EYLHSSHCSP AVNSTGEHTE
FSGDLDDGLF LISKLPKGSK WEVYPAQVQG PRTRKPPKGK RNENRATASS CNSPFPQRPR
KRLTEQELHD VSEKLSQRLS ELDWMLKSAL GDRIKEKTDH KEENTGNEEV EDGTEETLSQ
HSDGIVEYGP KKSRPGLSMR RKPPYRSHSL SPSPVNKHKQ FHLERKRQRK PRETDVRQFQ
AQAFTEAFER ELRRHKVQEN IGPLRIHEKE EETEKIYRGE AVRKGTPECS QPWKIYSRKT
TTQSLRGGLP KPNKAVPMKV SEHSLLPLML EQFPFLYVSG PTLSKMWKQQ IAQVEQLKKE
ACRENRSKKK LQDEIEEALR RHDLLTTLVK KEYEHNKRLQ DFKDCIRRQR LTQSKIKENR
QQIVRARKYY DDYRVQLCAK MMRMRTREEM IFKKLFEEGL NIQKQRLRDL RNYAKEKRDE
QRRRHQDELD SMENYYKDQF SLLAEAISQE HQELKAREKS QAQTLHKVKR ELRSKMEKEI
QQLQDMITQN DDDVFFRELE AERFRSRLQL ASFQYSKSPS L
