CER16_ARATH
ID CER16_ARATH Reviewed; 355 AA.
AC F4K8S5; Q7XA85; Q9FFH4;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Protein ECERIFERUM 16 {ECO:0000303|PubMed:31941670};
DE AltName: Full=RST1-intearcting protein {ECO:0000303|PubMed:31455787};
GN Name=CER16 {ECO:0000303|PubMed:31941670};
GN Synonyms=RIPR {ECO:0000303|PubMed:31455787};
GN OrderedLocusNames=At5g44150 {ECO:0000312|Araport:AT5G44150};
GN ORFNames=MLN1.7 {ECO:0000312|EMBL:BAB10982.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, INTERACTION WITH RST1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=31455787; DOI=10.1038/s41467-019-11807-4;
RA Lange H., Ndecky S.Y.A., Gomez-Diaz C., Pflieger D., Butel N., Zumsteg J.,
RA Kuhn L., Piermaria C., Chicher J., Christie M., Karaaslan E.S.,
RA Lang P.L.M., Weigel D., Vaucheret H., Hammann P., Gagliardi D.;
RT "RST1 and RIPR connect the cytosolic RNA exosome to the Ski complex in
RT Arabidopsis.";
RL Nat. Commun. 10:3871-3871(2019).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=31941670; DOI=10.1104/pp.19.01002;
RA Yang X., Feng T., Li S., Zhao H., Zhao S., Ma C., Jenks M.A., Lue S.;
RT "CER16 inhibits post-transcriptional gene silencing of CER3 to regulate
RT alkane biosynthesis.";
RL Plant Physiol. 182:1211-1221(2020).
CC -!- FUNCTION: Together with RST1, acts as cofactor of the cytoplasmic
CC exosome and connects the cytosolic RNA exosome to the SKI complex
CC (PubMed:31455787). Acts as post-transcriptional gene silencing (PTGS)
CC suppressor (PubMed:31455787, PubMed:31941670). CER16/RIPR can, like
CC RST1 suppress the production of small interfering RNAs (siRNAs) from
CC the CER3 locus, which is involved in cuticule membrane and wax
CC production, and in the typhine and sporopollenin biosynthesis of pollen
CC (PubMed:31455787, PubMed:31941670). {ECO:0000269|PubMed:31455787,
CC ECO:0000269|PubMed:31941670}.
CC -!- SUBUNIT: Interacts with RST1. {ECO:0000269|PubMed:31455787}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:31455787}.
CC Endoplasmic reticulum {ECO:0000269|PubMed:31941670}.
CC -!- TISSUE SPECIFICITY: Expressed in taproots, lateral roots, root tips,
CC leaf veins, cauline leaves, inflorescences, flowers, and siliques.
CC {ECO:0000269|PubMed:31941670}.
CC -!- DISRUPTION PHENOTYPE: Shrunken non viable seeds due to arrested embryo
CC development when homozygous (PubMed:31455787). Alteration in cuticular
CC waxes (PubMed:31455787, PubMed:31941670). Decreased production wax with
CC dramatically fewer alkanes (PubMed:31941670).
CC {ECO:0000269|PubMed:31455787, ECO:0000269|PubMed:31941670}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10982.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB005239; BAB10982.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED95067.1; -; Genomic_DNA.
DR EMBL; BT010142; AAQ22611.1; -; mRNA.
DR EMBL; AK227605; BAE99596.1; -; mRNA.
DR RefSeq; NP_199228.2; NM_123782.6.
DR AlphaFoldDB; F4K8S5; -.
DR STRING; 3702.AT5G44150.1; -.
DR PaxDb; F4K8S5; -.
DR PRIDE; F4K8S5; -.
DR ProteomicsDB; 212156; -.
DR EnsemblPlants; AT5G44150.1; AT5G44150.1; AT5G44150.
DR GeneID; 834438; -.
DR Gramene; AT5G44150.1; AT5G44150.1; AT5G44150.
DR KEGG; ath:AT5G44150; -.
DR Araport; AT5G44150; -.
DR TAIR; locus:2167598; AT5G44150.
DR eggNOG; ENOG502QPUK; Eukaryota.
DR HOGENOM; CLU_034598_1_0_1; -.
DR InParanoid; F4K8S5; -.
DR OrthoDB; 727876at2759; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4K8S5; baseline and differential.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Cytoplasm; Endoplasmic reticulum; Reference proteome.
FT CHAIN 1..355
FT /note="Protein ECERIFERUM 16"
FT /id="PRO_0000454454"
FT REGION 1..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 296..315
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 10..26
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 37..57
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 296..314
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 136
FT /note="R -> G (in Ref. 4; AAQ22611 and 5; BAE99596)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 355 AA; 39044 MW; 5A6825E8895D5721 CRC64;
MDSKSLAKSK RAHTLHHSKK SHSVHKPKVP GVSEKNPEKL QGNQTKSPVQ SRRVSALPSN
WDRYDDELDA AEDSSISLHS DVIVPKSKGA DYLHLISEAQ AESNSKIENN LDCLSSLDDL
LHDEFSRVVG SMISARGEGI LSWMEDDNFV VEEDGSGSYQ EPGFLSLNLN VLAKTLENVD
LHERLYIDPD LLPLPELNTS QTKVSRNEEP SHSHIAQNDP IVVPGESSVR EAESLDQVKD
ILILTDESEK SSAIEADLDL LLNSFSEAHT QPNPVASASG KSSAFETELD SLLKSHSSTE
QFNKPGNPSD QKIHMTGFND VLDDLLESTP VSIIPQSNQT SSKVLDDFDS WLDTI
