CER1_MOUSE
ID CER1_MOUSE Reviewed; 272 AA.
AC O55233; O35213;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Cerberus;
DE AltName: Full=Cerberus-like protein;
DE Short=Cer-l;
DE AltName: Full=Cerberus-related protein;
DE Flags: Precursor;
GN Name=Cer1; Synonyms=Cerl, Cerr1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=129/SvJ;
RX PubMed=9431803; DOI=10.1016/s0925-4773(97)00125-1;
RA Belo J.A., Bouwmeester T., Leyns L., Kertesz N., Gallo M., Follettie M.,
RA De Robertis E.M.;
RT "Cerberus-like is a secreted factor with neutralizing activity expressed in
RT the anterior primitive endoderm of the mouse gastrula.";
RL Mech. Dev. 68:45-57(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, AND
RP GLYCOSYLATION.
RX PubMed=9501024; DOI=10.1006/dbio.1997.8812;
RA Biben C., Stanley E., Fabri L., Kotecha S., Rhinn M., Drinkwater C.,
RA Lah M., Wang C.-C., Nash A., Hilton D., Ang S.-L., Mohun T., Harvey R.P.;
RT "Murine cerberus homologue mCer-1: a candidate anterior patterning
RT molecule.";
RL Dev. Biol. 194:135-151(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=9600941; DOI=10.1073/pnas.95.11.6198;
RA Shawlot W., Deng J.M., Behringer R.R.;
RT "Expression of the mouse cerberus-related gene, Cerr1, suggests a role in
RT anterior neural induction and somitogenesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:6198-6203(1998).
RN [4]
RP FUNCTION.
RX PubMed=12431380; DOI=10.1016/s1534-5807(02)00321-0;
RA Perea-Gomez A., Vella F.D., Shawlot W., Oulad-Abdelghani M., Chazaud C.,
RA Meno C., Pfister V., Chen L., Robertson E., Hamada H., Behringer R.R.,
RA Ang S.L.;
RT "Nodal antagonists in the anterior visceral endoderm prevent the formation
RT of multiple primitive streaks.";
RL Dev. Cell 3:745-756(2002).
CC -!- FUNCTION: Cytokine that may play a role in anterior neural induction
CC and somite formation during embryogenesis in part, through a BMP-
CC inhibitory mechanism. Can regulate Nodal signaling during gastrulation
CC as well as the formation and patterning of the primitive streak.
CC {ECO:0000269|PubMed:12431380, ECO:0000269|PubMed:9431803,
CC ECO:0000269|PubMed:9501024, ECO:0000269|PubMed:9600941}.
CC -!- SUBUNIT: Forms monomers and predominantly dimers.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9431803}.
CC -!- DEVELOPMENTAL STAGE: At 6.5 dpc, early and mid-streak stage embryos,
CC was expressed in the anterior visceral endoderm. The expression domain
CC extended from the extraembryonic-embryonic junction to approximately
CC two thirds of the way down the epiblast. By the mid-streak to late
CC streak stage, expressed in the anterior visceral endoderm but was also
CC expressed in the definitive endoderm emanating from the anterior
CC portion of the primitive streak. By the neural plate stage at 7.5 dpc
CC expression was present throughout the anterior definitive endoderm
CC layer including both the midline and anterior lateral endoderm. At the
CC early headfold stage expression was reduced in the anterior lateral
CC region and expression was seen primarily in the foregut endoderm. In
CC early 8.5 dpc embryos, expression was restricted to the two most
CC recently formed somites. In 9 dpc and 9.5 dpc embryos, expression
CC continued in the two newest formed somites and also in the anterior
CC presomitic mesoderm. {ECO:0000269|PubMed:9431803,
CC ECO:0000269|PubMed:9501024, ECO:0000269|PubMed:9600941}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:9501024}.
CC -!- SIMILARITY: Belongs to the DAN family. {ECO:0000305}.
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DR EMBL; AF012244; AAB71838.1; -; Genomic_DNA.
DR EMBL; AF035579; AAC02430.1; -; mRNA.
DR EMBL; AF031896; AAC24461.1; -; mRNA.
DR CCDS; CCDS18295.1; -.
DR RefSeq; NP_034017.1; NM_009887.2.
DR AlphaFoldDB; O55233; -.
DR BioGRID; 198677; 1.
DR STRING; 10090.ENSMUSP00000048607; -.
DR GlyGen; O55233; 2 sites.
DR iPTMnet; O55233; -.
DR PhosphoSitePlus; O55233; -.
DR PaxDb; O55233; -.
DR PRIDE; O55233; -.
DR Antibodypedia; 10019; 260 antibodies from 31 providers.
DR DNASU; 12622; -.
DR Ensembl; ENSMUST00000048430; ENSMUSP00000048607; ENSMUSG00000038192.
DR GeneID; 12622; -.
DR KEGG; mmu:12622; -.
DR UCSC; uc008tkl.2; mouse.
DR CTD; 9350; -.
DR MGI; MGI:1201414; Cer1.
DR VEuPathDB; HostDB:ENSMUSG00000038192; -.
DR eggNOG; ENOG502S2G4; Eukaryota.
DR GeneTree; ENSGT00530000063926; -.
DR HOGENOM; CLU_104447_0_0_1; -.
DR InParanoid; O55233; -.
DR OMA; FCSHCSP; -.
DR OrthoDB; 1134947at2759; -.
DR PhylomeDB; O55233; -.
DR TreeFam; TF106445; -.
DR Reactome; R-MMU-201451; Signaling by BMP.
DR BioGRID-ORCS; 12622; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Acer1; mouse.
DR PRO; PR:O55233; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; O55233; protein.
DR Bgee; ENSMUSG00000038192; Expressed in endoderm and 33 other tissues.
DR ExpressionAtlas; O55233; baseline and differential.
DR Genevisible; O55233; MM.
DR GO; GO:0005576; C:extracellular region; IDA:MGI.
DR GO; GO:0005615; C:extracellular space; IDA:BHF-UCL.
DR GO; GO:0036122; F:BMP binding; ISO:MGI.
DR GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR GO; GO:0016015; F:morphogen activity; ISO:MGI.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:BHF-UCL.
DR GO; GO:0009948; P:anterior/posterior axis specification; IDA:MGI.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IDA:MGI.
DR GO; GO:0030282; P:bone mineralization; ISO:MGI.
DR GO; GO:0042074; P:cell migration involved in gastrulation; IGI:MGI.
DR GO; GO:0008283; P:cell population proliferation; IGI:MGI.
DR GO; GO:0071773; P:cellular response to BMP stimulus; ISS:BHF-UCL.
DR GO; GO:0071276; P:cellular response to cadmium ion; IDA:MGI.
DR GO; GO:0048263; P:determination of dorsal identity; ISO:MGI.
DR GO; GO:0061371; P:determination of heart left/right asymmetry; IBA:GO_Central.
DR GO; GO:0007369; P:gastrulation; IGI:MGI.
DR GO; GO:0003419; P:growth plate cartilage chondrocyte proliferation; IEP:BHF-UCL.
DR GO; GO:0032926; P:negative regulation of activin receptor signaling pathway; IDA:BHF-UCL.
DR GO; GO:0030514; P:negative regulation of BMP signaling pathway; IDA:MGI.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IGI:MGI.
DR GO; GO:2000381; P:negative regulation of mesoderm development; IDA:BHF-UCL.
DR GO; GO:1900176; P:negative regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry; IBA:GO_Central.
DR GO; GO:0007399; P:nervous system development; ISO:MGI.
DR GO; GO:0035582; P:sequestering of BMP in extracellular matrix; IDA:BHF-UCL.
DR GO; GO:0023019; P:signal transduction involved in regulation of gene expression; IDA:MGI.
DR GO; GO:0001657; P:ureteric bud development; IMP:UniProtKB.
DR Gene3D; 2.10.90.10; -; 1.
DR InterPro; IPR016860; Cerberus.
DR InterPro; IPR006207; Cys_knot_C.
DR InterPro; IPR029034; Cystine-knot_cytokine.
DR InterPro; IPR004133; DAN.
DR PANTHER; PTHR15273; PTHR15273; 1.
DR Pfam; PF03045; DAN; 1.
DR PIRSF; PIRSF027807; Cerberus; 1.
DR SMART; SM00041; CT; 1.
DR PROSITE; PS01225; CTCK_2; 1.
PE 1: Evidence at protein level;
KW Cytokine; Disulfide bond; Glycoprotein; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000250"
FT CHAIN 18..272
FT /note="Cerberus"
FT /id="PRO_0000006712"
FT DOMAIN 162..246
FT /note="CTCK"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT CARBOHYD 168
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 222
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 162..209
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT DISULFID 176..223
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT DISULFID 186..239
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT DISULFID 190..241
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT CONFLICT 220
FT /note="M -> R (in Ref. 1; AAB71838)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 272 AA; 30431 MW; DD0842A2619AF057 CRC64;
MHLLLVQLLV LLPLGKADLC VDGCQSQGSL SFPLLERGRR DLHVANHEEA EDKPDLFVAV
PHLMGTSLAG EGQRQRGKML SRLGRFWKKP ETEFYPPRDV ESDHVSSGMQ AVTQPADGRK
VERSPLQEEA KRFWHRFMFR KGPAFQGVIL PIKSHEVHWE TCRTVPFNQT IAHEDCQKVV
VQNNLCFGKC SSIRFPGEGA DAHSFCSHCS PTKFTTVHLM LNCTSPTPVV KMVMQVEECQ
CMVKTERGEE RLLLAGSQGS FIPGLPASKT NP
