CER1_XENTR
ID CER1_XENTR Reviewed; 276 AA.
AC Q07G34; Q8AWG8;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 50.
DE RecName: Full=Cerberus;
DE Short=Cer;
DE AltName: Full=tCerberus;
DE Contains:
DE RecName: Full=Cerberus long;
DE Short=Cer-L;
DE Contains:
DE RecName: Full=Cerberus short;
DE Short=Cer-S;
DE Flags: Precursor;
GN Name=cer1 {ECO:0000312|EMBL:CAL49333.1}; ORFNames=TGas102k04.1;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAN76334.1}
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Gastrula {ECO:0000269|PubMed:12508233};
RX PubMed=12508233; DOI=10.1002/dvdy.10201;
RA D'Souza A., Lee M., Taverner N., Mason J., Carruthers S., Smith J.C.,
RA Amaya E., Papalopulu N., Zorn A.M.;
RT "Molecular components of the endoderm specification pathway in Xenopus
RT tropicalis.";
RL Dev. Dyn. 226:118-127(2003).
RN [2] {ECO:0000312|EMBL:CAL49333.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Gastrula {ECO:0000312|EMBL:CAL49333.1};
RG Sanger Xenopus tropicalis EST/cDNA project;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Inhibits wnt, nodal/nr-1 and bmp signaling in the embryo to
CC promote head formation and anterior neural induction. Within the
CC endoderm, acts as an essential mediator of nodal/nr-1-induced
CC cardiogenesis in the overlying mesoderm (By similarity).
CC {ECO:0000250|UniProtKB:P70041}.
CC -!- SUBUNIT: The long chain interacts with nodal/nr-1, bmp4 and wnt8,
CC thereby inhibiting their function. The short chain interacts with
CC nodal/nr-1 but not bmp4 or wnt8 (By similarity).
CC {ECO:0000250|UniProtKB:P70041}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in the anterior endomesoderm of the early
CC gastrula with expression expanded laterally around the margin at the
CC endoderm/mesoderm boundary. {ECO:0000269|PubMed:12508233}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the gastrula stage of embryos but
CC becomes absent by late neurula stage. {ECO:0000269|PubMed:12508233}.
CC -!- SIMILARITY: Belongs to the DAN family. {ECO:0000255}.
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DR EMBL; AY157640; AAN76334.1; -; mRNA.
DR EMBL; CR762343; CAL49333.1; -; mRNA.
DR RefSeq; NP_988846.1; NM_203515.1.
DR AlphaFoldDB; Q07G34; -.
DR GeneID; 394437; -.
DR KEGG; xtr:394437; -.
DR CTD; 9350; -.
DR Xenbase; XB-GENE-484971; cer1.
DR InParanoid; Q07G34; -.
DR OrthoDB; 1134947at2759; -.
DR Reactome; R-XTR-201451; Signaling by BMP.
DR Proteomes; UP000008143; Chromosome 1.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000038419; Expressed in gastrula and 3 other tissues.
DR GO; GO:0005576; C:extracellular region; ISS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0016015; F:morphogen activity; IBA:GO_Central.
DR GO; GO:0017147; F:Wnt-protein binding; ISS:UniProtKB.
DR GO; GO:0009948; P:anterior/posterior axis specification; ISS:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0061371; P:determination of heart left/right asymmetry; IBA:GO_Central.
DR GO; GO:0007507; P:heart development; ISS:UniProtKB.
DR GO; GO:0030514; P:negative regulation of BMP signaling pathway; ISS:UniProtKB.
DR GO; GO:1900176; P:negative regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry; IBA:GO_Central.
DR GO; GO:0030178; P:negative regulation of Wnt signaling pathway; ISS:UniProtKB.
DR GO; GO:0007399; P:nervous system development; ISS:UniProtKB.
DR GO; GO:0035582; P:sequestering of BMP in extracellular matrix; IBA:GO_Central.
DR GO; GO:0023019; P:signal transduction involved in regulation of gene expression; IBA:GO_Central.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR Gene3D; 2.10.90.10; -; 1.
DR InterPro; IPR016860; Cerberus.
DR InterPro; IPR006207; Cys_knot_C.
DR InterPro; IPR029034; Cystine-knot_cytokine.
DR InterPro; IPR004133; DAN.
DR PANTHER; PTHR15273; PTHR15273; 2.
DR Pfam; PF03045; DAN; 1.
DR SMART; SM00041; CT; 1.
DR PROSITE; PS01225; CTCK_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; Disulfide bond; Glycoprotein;
KW Neurogenesis; Reference proteome; Secreted; Signal; Wnt signaling pathway.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..276
FT /note="Cerberus long"
FT /id="PRO_0000273283"
FT CHAIN 110..270
FT /note="Cerberus short"
FT /evidence="ECO:0000250"
FT /id="PRO_0000273284"
FT DOMAIN 175..259
FT /note="CTCK"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT CARBOHYD 103
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 118
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 160
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 234
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 175..221
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT DISULFID 189..235
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT DISULFID 199..251
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT DISULFID 203..253
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT CONFLICT 53
FT /note="L -> V (in Ref. 1; AAN76334)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 276 AA; 31244 MW; 9555F2A75940962F CRC64;
MLLCVLKIYI IFCLVNDGAG KRSEVRGRTK TYSHNSRGYF RKERGMGKNK ILLVNTKGFD
QPHIGQGSFG LVAELFDSTR TDTSRKEPDI NKVKLFSTAA HANKSSRNIG IFRRKAFNGS
RRNIFSRQPF NKRNTDVTEK PGAKMFWNNF LVKMNGAPQN TSHGGKPQEI MKEACKTLPF
TQNIVHENCD RMVIQNNLCF GKCISLHVPN QQDRRNTCAH CLPSKFTLNH LALNCTGSNN
VVKVVMMVEE CACEAHKNNY HQTAQFNMDA STTLHN
