CERI1_PLAF7
ID CERI1_PLAF7 Reviewed; 446 AA.
AC O96188;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 2.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Rhoptry surface protein CERLI1 {ECO:0000305};
DE AltName: Full=Cytosolically exposed rhoptry leaflet interacting protein 1 {ECO:0000303|PubMed:32179747};
DE Short=PfCERLI1 {ECO:0000303|PubMed:32179747};
DE AltName: Full=Rhoptry apical surface protein 2 {ECO:0000303|PubMed:31492901};
DE Short=PfRASP2 {ECO:0000303|PubMed:31492901};
GN Name=CERLI1 {ECO:0000303|PubMed:32179747};
GN Synonyms=RASP2 {ECO:0000303|PubMed:31492901};
GN ORFNames=PF3D7_0210600 {ECO:0000312|EMBL:CZT98120.1};
OS Plasmodium falciparum (isolate 3D7).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=36329 {ECO:0000312|Proteomes:UP000001450};
RN [1] {ECO:0000312|Proteomes:UP000001450}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7 {ECO:0000312|Proteomes:UP000001450};
RX PubMed=9804551; DOI=10.1126/science.282.5391.1126;
RA Gardner M.J., Tettelin H., Carucci D.J., Cummings L.M., Aravind L.,
RA Koonin E.V., Shallom S.J., Mason T., Yu K., Fujii C., Pederson J., Shen K.,
RA Jing J., Aston C., Lai Z., Schwartz D.C., Pertea M., Salzberg S.L.,
RA Zhou L., Sutton G.G., Clayton R., White O., Smith H.O., Fraser C.M.,
RA Adams M.D., Venter J.C., Hoffman S.L.;
RT "Chromosome 2 sequence of the human malaria parasite Plasmodium
RT falciparum.";
RL Science 282:1126-1132(1998).
RN [2] {ECO:0000312|Proteomes:UP000001450}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7 {ECO:0000312|Proteomes:UP000001450};
RX PubMed=12368864; DOI=10.1038/nature01097;
RA Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W.,
RA Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D.,
RA Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S.,
RA Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M.,
RA Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A.,
RA Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I.,
RA Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J.,
RA Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G.;
RT "Genome sequence of the human malaria parasite Plasmodium falciparum.";
RL Nature 419:498-511(2002).
RN [3] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=31492901; DOI=10.1038/s41467-019-11979-z;
RA Suarez C., Lentini G., Ramaswamy R., Maynadier M., Aquilini E.,
RA Berry-Sterkers L., Cipriano M., Chen A.L., Bradley P., Striepen B.,
RA Boulanger M.J., Lebrun M.;
RT "A lipid-binding protein mediates rhoptry discharge and invasion in
RT Plasmodium falciparum and Toxoplasma gondii parasites.";
RL Nat. Commun. 10:4041-4041(2019).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=32179747; DOI=10.1038/s41467-020-15127-w;
RA Liffner B., Froelich S., Heinemann G.K., Liu B., Ralph S.A., Dixon M.W.A.,
RA Gilberger T.W., Wilson D.W.;
RT "PfCERLI1 is a conserved rhoptry associated protein essential for
RT Plasmodium falciparum merozoite invasion of erythrocytes.";
RL Nat. Commun. 11:1411-1411(2020).
CC -!- FUNCTION: Essential for merozoite invasion of host cells by controlling
CC rhoptry secretion (PubMed:31492901, PubMed:32179747). Binds to
CC phosphatidic acid (PA) and phosphatidylinositol 4,5-bisphosphate (PIP2)
CC lipids and thus, likely contributes to the assembly of the machinery
CC that docks or primes the rhoptry to the parasite cell membrane prior to
CC the fusion with the host cell membrane (PubMed:31492901).
CC {ECO:0000269|PubMed:31492901, ECO:0000269|PubMed:32179747}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, rhoptry
CC membrane {ECO:0000269|PubMed:31492901, ECO:0000269|PubMed:32179747};
CC Peripheral membrane protein {ECO:0000269|PubMed:32179747}; Cytoplasmic
CC side {ECO:0000269|PubMed:32179747}. Note=Localizes to the neck (apical
CC part) of rhoptries in merozoites (PubMed:31492901). In the mature
CC merozoite, localizes to the cytosolic face of the rhoptry bulb membrane
CC (PubMed:32179747). {ECO:0000269|PubMed:31492901,
CC ECO:0000269|PubMed:32179747}.
CC -!- DEVELOPMENTAL STAGE: During parasite asexual blood stages, expressed at
CC the late schizont stage and in free merozoites (at protein level).
CC {ECO:0000269|PubMed:31492901, ECO:0000269|PubMed:32179747}.
CC -!- DOMAIN: The C2 domain is a non-calcium binding domain (By similarity).
CC Cooperates with the PH domain in the binding to phosphatidic acid (PA)
CC and phosphatidylinositol 4,5-bisphosphate (PIP2) (By similarity).
CC {ECO:0000250|UniProtKB:S7UMJ0}.
CC -!- DOMAIN: The PH domain cooperates with the C2 domain in the binding to
CC phosphatidic acid (PA) and phosphatidylinositol 4,5-bisphosphate
CC (PIP2). {ECO:0000250|UniProtKB:S7UMJ0}.
CC -!- DISRUPTION PHENOTYPE: Conditional knockout at the ring stage has no
CC effect on parasite development during the first cycle and merozoites
CC egress normally from host erythrocytes. However, these merozoites are
CC unable to reinvade new erythrocytes (PubMed:31492901, PubMed:32179747).
CC Impaired rhoptry secretion during merozoite invasion of host
CC erythrocytes (PubMed:31492901, PubMed:32179747). Increases the spatial
CC segregation between rhoptry neck protein RON4 and bulb protein RAP1,
CC and impairs RAP1 processing (PubMed:32179747). No effect on rhoptry
CC morphology (PubMed:31492901, PubMed:32179747). Microneme secretion is
CC not affected (PubMed:31492901). {ECO:0000269|PubMed:31492901,
CC ECO:0000269|PubMed:32179747}.
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DR EMBL; LN999943; CZT98120.1; -; Genomic_DNA.
DR RefSeq; XP_001349613.1; XM_001349577.1.
DR AlphaFoldDB; O96188; -.
DR STRING; 5833.PFB0475c; -.
DR SwissPalm; O96188; -.
DR PRIDE; O96188; -.
DR EnsemblProtists; CZT98120; CZT98120; PF3D7_0210600.
DR GeneID; 812695; -.
DR KEGG; pfa:PF3D7_0210600; -.
DR VEuPathDB; PlasmoDB:PF3D7_0210600; -.
DR HOGENOM; CLU_614653_0_0_1; -.
DR InParanoid; O96188; -.
DR OMA; IHHINLK; -.
DR PhylomeDB; O96188; -.
DR Proteomes; UP000001450; Chromosome 2.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Membrane; Reference proteome.
FT CHAIN 1..446
FT /note="Rhoptry surface protein CERLI1"
FT /id="PRO_0000456207"
FT DOMAIN 39..208
FT /note="C2"
FT /evidence="ECO:0000250|UniProtKB:S7UMJ0"
FT DOMAIN 252..363
FT /note="PH"
FT /evidence="ECO:0000250|UniProtKB:S7UMJ0"
SQ SEQUENCE 446 AA; 53675 MW; C67972E6A84F6572 CRC64;
MFYLDTHNIL YLGSCLLASV CTLCICRNRE LFPHISENKP IGQLYRLMNI HKYESFSIII
QIHHLNLKFG DDDNAKFIVH LKIGNRYAYT HYHKQYQNKV HIEERKNMVV KQNNNTLRLE
VYKKGTLKNT FFGSAEIHIY SEIVKKLFPC NVYFNITNKN QIVGTACLSF HYINLDCIKK
DDQIYTSLFI ETIISVQKNQ TKNNEKIEKL IDEGKEHFEA IKETDLSTTI YKNISNLVLE
DKIRLFCKNL NGYLLHSNFY IKRFYNKYYF YLHFFKGKFY WCYYNEEADA KMDKNRVGYV
RLEYVANVYS DVYSHKYFYI KYRKKNERKE NYLYLKTIDK DRNIWVNIIH DFIILVSNYK
RERKNKKYKI KEFKDNLIED TPKEILEINK TLSRSLSNNS MKNKYLDKKK KVEVLSDMDN
EENYMNSGDL GPVFKDMSKN MYNYSD
