CERI2_PLAF7
ID CERI2_PLAF7 Reviewed; 579 AA.
AC Q8I1X7;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Rhoptry surface protein CERLI2 {ECO:0000305};
DE AltName: Full=Ag-1 blood stage membrane protein homolog {ECO:0000303|PubMed:19777263};
DE Short=Pfmag-1 {ECO:0000303|PubMed:19777263};
DE AltName: Full=Cytosolically exposed rhoptry leaflet interacting protein 2 {ECO:0000303|PubMed:35140336};
DE Short=PfCERLI2 {ECO:0000303|PubMed:35140336};
GN Name=CERLI2 {ECO:0000303|PubMed:35140336};
GN Synonyms=MAG-1 {ECO:0000303|PubMed:19777263};
GN ORFNames=PF3D7_0405200 {ECO:0000312|EMBL:CAD49144.1};
OS Plasmodium falciparum (isolate 3D7).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=36329 {ECO:0000312|Proteomes:UP000001450};
RN [1] {ECO:0000312|Proteomes:UP000001450}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7 {ECO:0000312|Proteomes:UP000001450};
RX PubMed=12368864; DOI=10.1038/nature01097;
RA Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W.,
RA Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D.,
RA Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S.,
RA Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M.,
RA Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A.,
RA Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I.,
RA Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J.,
RA Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G.;
RT "Genome sequence of the human malaria parasite Plasmodium falciparum.";
RL Nature 419:498-511(2002).
RN [2] {ECO:0000312|Proteomes:UP000001450}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7 {ECO:0000312|Proteomes:UP000001450};
RX PubMed=12368867; DOI=10.1038/nature01095;
RA Hall N., Pain A., Berriman M., Churcher C.M., Harris B., Harris D.,
RA Mungall K.L., Bowman S., Atkin R., Baker S., Barron A., Brooks K.,
RA Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T.,
RA Christodoulou Z., Clark L., Clark R., Corton C., Cronin A., Davies R.M.,
RA Davis P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A.,
RA Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H.,
RA Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James K.D.,
RA Johnson D., Kerhornou A., Knights A., Konfortov B., Kyes S., Larke N.,
RA Lawson D., Lennard N., Line A., Maddison M., Mclean J., Mooney P.,
RA Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A.,
RA Rabbinowitsch E., Rajandream M.A., Rutter S., Rutherford K.M., Sanders M.,
RA Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S.,
RA Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.R.,
RA Sulston J.E., Craig A., Newbold C., Barrell B.G.;
RT "Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.";
RL Nature 419:527-531(2002).
RN [3] {ECO:0000305}
RP SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, POLYMORPHISM, BIOTECHNOLOGY, AND
RP REPEATS.
RX PubMed=19777263; DOI=10.1007/s00436-009-1617-4;
RA Gao Y.H., Li H.L., Lu Y., Gao F.M., Lin Y.H., Zhou H.C., Zhang L.H.,
RA Wang H.;
RT "Identification of a vaccine candidate antigen, PfMAg-1, from Plasmodium
RT falciparum with monoclonal antibody M26-32.";
RL Parasitol. Res. 105:1723-1732(2009).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=35140336; DOI=10.1038/s42003-022-03020-9;
RA Liffner B., Balbin J.M., Shami G.J., Siddiqui G., Strauss J., Froelich S.,
RA Heinemann G.K., Edwards E.M., Alder A., Wichers J.S., Creek D.J.,
RA Tilley L., Dixon M.W.A., Gilberger T.W., Wilson D.W.;
RT "Cell biological analysis reveals an essential role for Pfcerli2 in
RT erythrocyte invasion by malaria parasites.";
RL Commun. Biol. 5:121-121(2022).
CC -!- FUNCTION: Plays an important role in rhoptry physiology and thus is
CC essential for merozoite invasion of host erythrocytes.
CC {ECO:0000269|PubMed:35140336}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, rhoptry
CC membrane {ECO:0000269|PubMed:35140336}; Peripheral membrane protein
CC {ECO:0000269|PubMed:35140336}; Cytoplasmic side
CC {ECO:0000269|PubMed:35140336}. Cell membrane
CC {ECO:0000269|PubMed:19777263}; Peripheral membrane protein
CC {ECO:0000269|PubMed:19777263}. Host cell membrane
CC {ECO:0000269|PubMed:19777263}; Peripheral membrane protein
CC {ECO:0000269|PubMed:19777263}; Cytoplasmic side
CC {ECO:0000269|PubMed:19777263}. Note=Localizes below the host
CC erythrocyte cell membrane (PubMed:19777263). Localizes to the cell
CC membrane of merozoites (PubMed:19777263). In merozoites, localizes to
CC the cytosolic face of the rhoptry bulb membrane (PubMed:35140336).
CC {ECO:0000269|PubMed:19777263, ECO:0000269|PubMed:35140336}.
CC -!- DEVELOPMENTAL STAGE: During parasite asexual blood stages, expressed in
CC trophozoites and at the schizont stage (at protein level).
CC {ECO:0000269|PubMed:19777263}.
CC -!- POLYMORPHISM: The number of deca-peptide repeats in the C-terminus
CC varies across P.falciparum strains (PubMed:19777263). Strain 3D7 has 12
CC copies; Dd2 has 14 copies; Hondura-1, FCC1, FCC2, and T996 have 17
CC copies; and strain D10 and FCR3 have 19 copies (PubMed:19777263).
CC {ECO:0000269|PubMed:19777263}.
CC -!- DISRUPTION PHENOTYPE: Impairs merozoite invasion of host erythrocytes
CC at, or prior to, the formation of the tight junction (PubMed:35140336).
CC Merozoite development and egress following schizont rupture are not
CC affected (PubMed:35140336). Inhibits rhoptry bulb RAP1 processing but
CC not secretion of rhoptry neck proteins RH4 and RON4 (PubMed:35140336).
CC Causes defects in rhoptry morphology which are narrower and more
CC elongated (PubMed:35140336). No defect in microneme secretion
CC (PubMed:35140336). {ECO:0000269|PubMed:35140336}.
CC -!- BIOTECHNOLOGY: Antibodies against CERLI2 severely reduces parasitemia,
CC suggesting that CERLI2 is a potential vaccine candidate.
CC {ECO:0000269|PubMed:19777263}.
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DR EMBL; AL844503; CAD49144.1; -; Genomic_DNA.
DR RefSeq; XP_001351364.1; XM_001351328.1.
DR AlphaFoldDB; Q8I1X7; -.
DR SMR; Q8I1X7; -.
DR IntAct; Q8I1X7; 2.
DR STRING; 5833.PFD0255w; -.
DR PRIDE; Q8I1X7; -.
DR EnsemblProtists; CAD49144; CAD49144; PF3D7_0405200.
DR GeneID; 812431; -.
DR KEGG; pfa:PF3D7_0405200; -.
DR VEuPathDB; PlasmoDB:PF3D7_0405200; -.
DR HOGENOM; CLU_033873_0_0_1; -.
DR InParanoid; Q8I1X7; -.
DR OMA; EPFASMY; -.
DR PhylomeDB; Q8I1X7; -.
DR Proteomes; UP000001450; Chromosome 4.
PE 1: Evidence at protein level;
KW Cell membrane; Cytoplasmic vesicle; Host cell membrane; Host membrane;
KW Membrane; Reference proteome; Repeat.
FT CHAIN 1..579
FT /note="Rhoptry surface protein CERLI2"
FT /id="PRO_0000456208"
FT DOMAIN 52..84
FT /note="C2"
FT /evidence="ECO:0000305|PubMed:35140336"
FT REPEAT 442..451
FT /note="1"
FT /evidence="ECO:0000269|PubMed:19777263"
FT REPEAT 452..461
FT /note="2"
FT /evidence="ECO:0000269|PubMed:19777263"
FT REPEAT 462..471
FT /note="3"
FT /evidence="ECO:0000269|PubMed:19777263"
FT REPEAT 472..481
FT /note="4"
FT /evidence="ECO:0000269|PubMed:19777263"
FT REPEAT 482..491
FT /note="5"
FT /evidence="ECO:0000269|PubMed:19777263"
FT REPEAT 492..501
FT /note="6"
FT /evidence="ECO:0000269|PubMed:19777263"
FT REPEAT 502..511
FT /note="7"
FT /evidence="ECO:0000269|PubMed:19777263"
FT REPEAT 522..531
FT /note="9"
FT /evidence="ECO:0000269|PubMed:19777263"
FT REPEAT 532..541
FT /note="10"
FT /evidence="ECO:0000269|PubMed:19777263"
FT REPEAT 542..551
FT /note="11"
FT /evidence="ECO:0000269|PubMed:19777263"
FT REPEAT 552..561
FT /note="12"
FT /evidence="ECO:0000269|PubMed:19777263"
FT REGION 442..561
FT /note="12 X 10 AA tandem repeat of Q-T-E-I-[K/N]-N-D-
FT [H/N/I][I/N]"
FT /evidence="ECO:0000269|PubMed:19777263"
SQ SEQUENCE 579 AA; 69103 MW; 85252296941E4D49 CRC64;
MLGTKFILNI FKNKFNNLST RFIKYVIKSY KRTCPHISDF EPFSSMYYYT GLHNYRTFYL
LIKINEIFNI NKYKQIYIIV NTDKYDFTTD DIETNKKNRI YLDQRVDIKI RQCDEVIRIN
LFTTKLTKKV HIGEIKIDIN ASIISKCFPK NEWFVCIKDG QEVCKVQLSF YKTQKYACPS
ECMFIHDGLE IWKDKSKSGS TKKIDINNIH KNFDDNDEHL AMIDIDTDIN DISLIQKLKL
ISFVLEQEIC VFDFYAYVLK YMYAKEIMGE WFLCFLCFKN NKDQEEINIE TIIERNIHRD
INIPMLNIQR IVVDKRNNTN ANIIYTYQDE KYTLSIHSKK NLYYIIDAIT IFTNDLKILK
ESFGDELEYR KNIIMKENTM KKLMGKRRLS SPRFRKNLPK STHKMIKEMY DEQDCSHDVK
ESNTQFFEND MKTNEIKNDN IQTDEIKNDN IQTDEIKNDH IQTDEIKNDN IQTDEIKNDN
IQTDEIKNDH IQTDEIKNDN IQTDEIKNDN IQTDEIKNDH IQTDEINNDH IQTDEIKNDH
IQTDEIKNDH IQTDEIKNDI NTSNEIFFKA QLENTIQNI
